Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1986 Dec;125(3):460–464.

Amyloid P component is not present in the glomerular basement membrane in Alport-type hereditary nephritis.

T Melvin, Y Kim, A F Michael
PMCID: PMC1888458  PMID: 3541639

Abstract

Serum amyloid P component (SAP) is a normal plasma glycoprotein immunochemically indistinguishable from amyloid P, a glycoprotein found in all tissue amyloid deposits. SAP has also been shown to be a constituent of normal glomerular basement membrane (GBM). In this study the authors discovered a unique association between SAP and Goodpasture (GP) antigen. In those patients whose GBM lack GP antigen (those with Alport-type hereditary nephritis) SAP is also uniformly absent. Although the relationship between these two components is unknown, this association may provide clues to the abnormality of GBM in Alport-type hereditary nephritis.

Full text

PDF
460

Images in this article

462Figure A
on p.462

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  2. Butkowski R. J., Wieslander J., Wisdom B. J., Barr J. F., Noelken M. E., Hudson B. G. Properties of the globular domain of type IV collagen and its relationship to the Goodpasture antigen. J Biol Chem. 1985 Mar 25;260(6):3739–3747. [PubMed] [Google Scholar]
  3. Dyck R. F., Lockwood C. M., Kershaw M., McHugh N., Duance V. C., Baltz M. L., Pepys M. B. Amyloid P-component is a constituent of normal human glomerular basement membrane. J Exp Med. 1980 Nov 1;152(5):1162–1174. doi: 10.1084/jem.152.5.1162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fish A. J., Lockwood M. C., Wong M., Price R. G. Detection of Goodpasture antigen in fractions prepared from collagenase digests of human glomerular basement membrane. Clin Exp Immunol. 1984 Jan;55(1):58–66. [PMC free article] [PubMed] [Google Scholar]
  5. Hind C. R., Collins P. M., Renn D., Cook R. B., Caspi D., Baltz M. L., Pepys M. B. Binding specificity of serum amyloid P component for the pyruvate acetal of galactose. J Exp Med. 1984 Apr 1;159(4):1058–1069. doi: 10.1084/jem.159.4.1058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jenis E. H., Valeski J. E., Calcagno P. L. Variability of anti-GBM binding in hereditary nephritis. Clin Nephrol. 1981 Mar;15(3):111–114. [PubMed] [Google Scholar]
  7. Jeraj K., Kim Y., Vernier R. L., Fish A. J., Michael A. F. Absence of Goodpasture's antigen in male patients with familial nephritis. Am J Kidney Dis. 1983 May;2(6):626–629. doi: 10.1016/s0272-6386(83)80043-2. [DOI] [PubMed] [Google Scholar]
  8. Ku C. S., Fiedel B. A. Modulation of fibrin clot formation by human serum amyloid P component (SAP) and heparin. J Exp Med. 1983 Sep 1;158(3):767–780. doi: 10.1084/jem.158.3.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Melvin T. R., Kim Y., Conley M. E., Delacroix D. L., Michael A. F. Hidden IgA subclass determinants in human renal disease. Kidney Int. 1985 Apr;27(4):685–689. doi: 10.1038/ki.1985.65. [DOI] [PubMed] [Google Scholar]
  11. Melvin T., Kim Y., Michael A. F. Selective binding of IgG4 and other negatively charged plasma proteins in normal and diabetic human kidneys. Am J Pathol. 1984 Jun;115(3):443–446. [PMC free article] [PubMed] [Google Scholar]
  12. Olson D. L., Anand S. K., Landing B. H., Heuser E., Grushkin C. M., Lieberman E. Diagnosis of hereditary nephritis by failure of glomeruli to bind anti-glomerular basement membrane antibodies. J Pediatr. 1980 Apr;96(4):697–699. doi: 10.1016/s0022-3476(80)80746-3. [DOI] [PubMed] [Google Scholar]
  13. Osmand A. P., Friedenson B., Gewurz H., Painter R. H., Hofmann T., Shelton E. Characterization of C-reactive protein and the complement subcomponent C1t as homologous proteins displaying cyclic pentameric symmetry (pentraxins). Proc Natl Acad Sci U S A. 1977 Feb;74(2):739–743. doi: 10.1073/pnas.74.2.739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pepys M. B., Dash A. C., Ashley M. J. Isolation of C-reactive protein by affinity chromatography. Clin Exp Immunol. 1977 Oct;30(1):32–37. [PMC free article] [PubMed] [Google Scholar]
  15. Platt J. L., LeBien T. W., Michael A. F. Interstitial mononuclear cell populations in renal graft rejection. Identification by monoclonal antibodies in tissue sections. J Exp Med. 1982 Jan 1;155(1):17–30. doi: 10.1084/jem.155.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Scheinman J. I., Tsai C. Monoclonal antibody to type IV collagen with selective basement membrane localization. Lab Invest. 1984 Jan;50(1):101–112. [PubMed] [Google Scholar]
  17. Wieslander J., Barr J. F., Butkowski R. J., Edwards S. J., Bygren P., Heinegård D., Hudson B. G. Goodpasture antigen of the glomerular basement membrane: localization to noncollagenous regions of type IV collagen. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3838–3842. doi: 10.1073/pnas.81.12.3838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yoshioka K., Kleppel M., Fish A. J. Analysis of nephritogenic antigens in human glomerular basement membrane by two-dimensional gel electrophoresis. J Immunol. 1985 Jun;134(6):3831–3837. [PubMed] [Google Scholar]
  19. Yoshioka K., Michael A. F., Velosa J., Fish A. J. Detection of hidden nephritogenic antigen determinants in human renal and nonrenal basement membranes. Am J Pathol. 1985 Oct;121(1):156–165. [PMC free article] [PubMed] [Google Scholar]
  20. de Beer F. C., Baltz M. L., Holford S., Feinstein A., Pepys M. B. Fibronectin and C4-binding protein are selectively bound by aggregated amyloid P component. J Exp Med. 1981 Oct 1;154(4):1134–1139. doi: 10.1084/jem.154.4.1134. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES