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. 1995 Apr;69(4):2674–2678. doi: 10.1128/jvi.69.4.2674-2678.1995

Varicella-zoster virus (VZV) transcription during latency in human ganglia: prevalence of VZV gene 21 transcripts in latently infected human ganglia.

R J Cohrs 1, M B Barbour 1, R Mahalingam 1, M Wellish 1, D H Gilden 1
PMCID: PMC188953  PMID: 7884921

Abstract

Reverse transcriptase-linked PCR was used to determine the prevalence of varicella-zoster virus (VZV) gene 21 transcription in latently infected human ganglia. Under conditions wherein reverse transcriptase-linked PCR detected > or = 1,000 transcripts, VZV gene 21 RNA, but not VZV gene 40 RNA, was found in ganglia but not other tissues from five of seven humans.

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Selected References

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  1. Albright A. G., Jenkins F. J. The herpes simplex virus UL37 protein is phosphorylated in infected cells. J Virol. 1993 Aug;67(8):4842–4847. doi: 10.1128/jvi.67.8.4842-4847.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen J. I., Heffel D., Seidel K. The transcriptional activation domain of varicella-zoster virus open reading frame 62 protein is not conserved with its herpes simplex virus homolog. J Virol. 1993 Jul;67(7):4246–4251. doi: 10.1128/jvi.67.7.4246-4251.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohrs R. J., Goswami B. B., Sharma O. K. Occurrence of 2-5A and RNA degradation in the chick oviduct during rapid estrogen withdrawal. Biochemistry. 1988 May 3;27(9):3246–3252. doi: 10.1021/bi00409a019. [DOI] [PubMed] [Google Scholar]
  4. Cohrs R. J., Srock K., Barbour M. B., Owens G., Mahalingam R., Devlin M. E., Wellish M., Gilden D. H. Varicella-zoster virus (VZV) transcription during latency in human ganglia: construction of a cDNA library from latently infected human trigeminal ganglia and detection of a VZV transcript. J Virol. 1994 Dec;68(12):7900–7908. doi: 10.1128/jvi.68.12.7900-7908.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohrs R., Mahalingam R., Dueland A. N., Wolf W., Wellish M., Gilden D. H. Restricted transcription of varicella-zoster virus in latently infected human trigeminal and thoracic ganglia. J Infect Dis. 1992 Aug;166 (Suppl 1):S24–S29. doi: 10.1093/infdis/166.supplement_1.s24. [DOI] [PubMed] [Google Scholar]
  6. Doerig C., Pizer L. I., Wilcox C. L. An antigen encoded by the latency-associated transcript in neuronal cell cultures latently infected with herpes simplex virus type 1. J Virol. 1991 May;65(5):2724–2727. doi: 10.1128/jvi.65.5.2724-2727.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Forghani B., Dupuis K. W., Schmidt N. J. Varicella-zoster viral glycoproteins analyzed with monoclonal antibodies. J Virol. 1984 Oct;52(1):55–62. doi: 10.1128/jvi.52.1.55-62.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kinchington P. R., Hougland J. K., Arvin A. M., Ruyechan W. T., Hay J. The varicella-zoster virus immediate-early protein IE62 is a major component of virus particles. J Virol. 1992 Jan;66(1):359–366. doi: 10.1128/jvi.66.1.359-366.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kutish G., Mainprize T., Rock D. Characterization of the latency-related transcriptionally active region of the bovine herpesvirus 1 genome. J Virol. 1990 Dec;64(12):5730–5737. doi: 10.1128/jvi.64.12.5730-5737.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lokensgard J. R., Thawley D. G., Molitor T. W. Pseudorabies virus latency: restricted transcription. Arch Virol. 1990;110(1-2):129–136. doi: 10.1007/BF01310709. [DOI] [PubMed] [Google Scholar]
  11. Mahalingam R., Smith D., Wellish M., Wolf W., Dueland A. N., Cohrs R., Soike K., Gilden D. Simian varicella virus DNA in dorsal root ganglia. Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2750–2752. doi: 10.1073/pnas.88.7.2750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mahalingam R., Wellish M., Wolf W., Dueland A. N., Cohrs R., Vafai A., Gilden D. Latent varicella-zoster viral DNA in human trigeminal and thoracic ganglia. N Engl J Med. 1990 Sep 6;323(10):627–631. doi: 10.1056/NEJM199009063231002. [DOI] [PubMed] [Google Scholar]
  13. McLauchlan J., Liefkens K., Stow N. D. The herpes simplex virus type 1 UL37 gene product is a component of virus particles. J Gen Virol. 1994 Aug;75(Pt 8):2047–2052. doi: 10.1099/0022-1317-75-8-2047. [DOI] [PubMed] [Google Scholar]
  14. Meier J. L., Holman R. P., Croen K. D., Smialek J. E., Straus S. E. Varicella-zoster virus transcription in human trigeminal ganglia. Virology. 1993 Mar;193(1):193–200. doi: 10.1006/viro.1993.1115. [DOI] [PubMed] [Google Scholar]
  15. Meier J. L., Straus S. E. Varicella-zoster virus DNA polymerase and major DNA-binding protein genes have overlapping divergent promoters. J Virol. 1993 Dec;67(12):7573–7581. doi: 10.1128/jvi.67.12.7573-7581.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nakajima-Iijima S., Hamada H., Reddy P., Kakunaga T. Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6133–6137. doi: 10.1073/pnas.82.18.6133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ng T. I., Keenan L., Kinchington P. R., Grose C. Phosphorylation of varicella-zoster virus open reading frame (ORF) 62 regulatory product by viral ORF 47-associated protein kinase. J Virol. 1994 Mar;68(3):1350–1359. doi: 10.1128/jvi.68.3.1350-1359.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Perera L. P., Mosca J. D., Ruyechan W. T., Hayward G. S., Straus S. E., Hay J. A major transactivator of varicella-zoster virus, the immediate-early protein IE62, contains a potent N-terminal activation domain. J Virol. 1993 Aug;67(8):4474–4483. doi: 10.1128/jvi.67.8.4474-4483.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Priola S. A., Gustafson D. P., Wagner E. K., Stevens J. G. A major portion of the latent pseudorabies virus genome is transcribed in trigeminal ganglia of pigs. J Virol. 1990 Oct;64(10):4755–4760. doi: 10.1128/jvi.64.10.4755-4760.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rogers R. P., Strominger J. L., Speck S. H. Epstein-Barr virus in B lymphocytes: viral gene expression and function in latency. Adv Cancer Res. 1992;58:1–26. doi: 10.1016/s0065-230x(08)60288-2. [DOI] [PubMed] [Google Scholar]
  21. Shelton L. S., Pensiero M. N., Jenkins F. J. Identification and characterization of the herpes simplex virus type 1 protein encoded by the UL37 open reading frame. J Virol. 1990 Dec;64(12):6101–6109. doi: 10.1128/jvi.64.12.6101-6109.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stevens J. G. Human herpesviruses: a consideration of the latent state. Microbiol Rev. 1989 Sep;53(3):318–332. doi: 10.1128/mr.53.3.318-332.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stevens J. G., Wagner E. K., Devi-Rao G. B., Cook M. L., Feldman L. T. RNA complementary to a herpesvirus alpha gene mRNA is prominent in latently infected neurons. Science. 1987 Feb 27;235(4792):1056–1059. doi: 10.1126/science.2434993. [DOI] [PubMed] [Google Scholar]
  24. Tyler J. K., Everett R. D. The DNA binding domain of the varicella-zoster virus gene 62 protein interacts with multiple sequences which are similar to the binding site of the related protein of herpes simplex virus type 1. Nucleic Acids Res. 1993 Feb 11;21(3):513–522. doi: 10.1093/nar/21.3.513. [DOI] [PMC free article] [PubMed] [Google Scholar]

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