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. 1995 May;69(5):3233–3236. doi: 10.1128/jvi.69.5.3233-3236.1995

Different roles of bases within the integration signal sequence of human immunodeficiency virus type 1 in vitro.

T Yoshinaga 1, T Fujiwara 1
PMCID: PMC189032  PMID: 7707558

Abstract

To investigate the roles of bases near the tips of each strand of the long terminal repeat of the human immunodeficiency virus type 1 in the integration reaction, we examined the efficiencies of both binding and integration activities of staggered-ended substrates and mismatched mutant substrates by the integration assay and the UV cross-linking assay. Our results suggest that some bases of the human immunodeficiency virus type 1 long terminal repeat are required primarily for binding, whereas others are more critical for later reaction steps in vitro.

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Selected References

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