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. 1995 Jul;69(7):4029–4036. doi: 10.1128/jvi.69.7.4029-4036.1995

Illegitimate replication of linear hepadnavirus DNA through nonhomologous recombination.

W Yang 1, J Summers 1
PMCID: PMC189136  PMID: 7769660

Abstract

Linear hepadnavirus DNA in primary hepatocyte cultures efficiently participates in intra- and intermolecular nonhomologous recombination at its ends. The products of this recombination are (i) monomeric covalently closed circular DNAs (cccDNAs) with deletions and insertions around the site of joining and (ii) oligomeric forms in which monomers are joined near the ends in random orientation. A fraction of monomeric cccDNAs can serve as intermediates in further DNA replication through at least five generations of nonhomologous recombination in a process we call illegitimate replication. We suggest that the monomeric and oligomeric linear DNAs produced by illegitimate replication may be precursors of the integrated and other high-molecular-weight hepadnaviral DNA forms seen in chronic infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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