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. 1997 Aug;41(2):215–220. doi: 10.1136/gut.41.2.215

T cells of the human intestinal lamina propria are high producers of interleukin-10

J Braunstein 1, L Qiao 1, F Autschbach 1, G Schurmann 1, S Meuer 1
PMCID: PMC1891463  PMID: 9301501

Abstract

Background and aim—Some of the recently observed functional features characteristic of immunocompetent cells residing in the human intestinal lamina propria could be mediated by interleukin- 10 (IL-10). To investigate the role of IL-10 in the human intestinal mucosa, the regulation of IL-10 production by lamina propria T lymphocytes (LPL-T) was determined and compared with that of peripheral blood T lymphocytes (PBL-T). 
Methods—Following activation by using different stimuli, IL-10 release by LPL-T and PBL-T into the supernatant was measured by enzyme linked immunosorbent assay (ELISA). In parallel, cell growth was determined by [3H]-thymidine incorporation. 
Results—Neither LPL-T nor PBL-T release IL-10 constitutively. Triggering through CD2 or the T cell receptor (TCR)/CD3 complex in the presence of autologous monocytes induces significantly greater IL-10 secretion by LPL-T than by PBL-T. Engagement of the CD45 receptor enhances IL-10 release and proliferation of CD2 triggered CD45RO+ PBL-T. In contrast, it reduces CD2 induced IL-10 production by LPL-T without altering cell growth significantly. 
Conclusions—Activated LPL-T release relatively high amounts of IL-10. Enhanced IL-10 production by activated LPL-T, in comparison with activated PBL-T, is not only related to the presence of a higher proportion of CD45RO+ T cells in the intestinal lamina propria, but is also caused by increased sensitivity of LPL-T to CD2 co-stimulation. The differential responsiveness of LPL-T, compared with PBL-T, to CD45 engagement demonstrates that CD45 could be involved in the altered CD2 reactivity of LPL-T. 



Keywords: CD2; CD45; interleukin 10; lamina propria; T cell subsets; T lymphocytes

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Figure 1 .

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: PBL-T and LPL-T were activated by CD2 monoclonal antibodies T112 and T113 (1/1000 v/v) in the absence (open columns) or presence (shaded columns) of CD45 monoclonal antibody AlCD45.1/B211 (1/400 v/v). (A) Supernatants were harvested after 60 hours and IL-10 content (results expressed in pg/ml) were determined by using ELISA. (B) Cell growth was measured by incorporation of [3H]-thymidine after four days. Results are expressed as the mean cpm of triplicate cultures. SD < 20%. The results shown are representative of four different experiments. 


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