Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1995 Jul;69(7):4495–4499. doi: 10.1128/jvi.69.7.4495-4499.1995

A herpesvirus saimiri protein required for interleukin-2 independence is associated with membranes of transformed T cells.

T Lund 1, M M Medveczky 1, P Geck 1, P G Medveczky 1
PMCID: PMC189192  PMID: 7769710

Abstract

A region of the herpesvirus saimiri genome encoding an mRNA with two open reading frames (ORFs) has been identified to be essential for transformation of T cells. Deletion of either ORF resulted in the loss of transforming ability. ORF-1 has been shown to code for a collagen-like oncoprotein. This study shows for the first time that the bicistronic mRNA can translate a 32-kDa protein from ORF-2. Polyclonal serum to ORF-2 was generated by using a glutathione fusion protein. Using this antiserum, ORF-2 was localized in cell membranes and is expressed on the outer cell membrane. The half-life of this membrane protein was found to be about 5.5 h. Limited sequence similarity was found between ORF-2 and interleukin-11; however, no secretion of ORF-2 protein was detected in supernatants from transformed cells. Further studies are required to investigate the potential interaction with the interleukin-11 receptor.

Full Text

The Full Text of this article is available as a PDF (324.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biesinger B., Müller-Fleckenstein I., Simmer B., Lang G., Wittmann S., Platzer E., Desrosiers R. C., Fleckenstein B. Stable growth transformation of human T lymphocytes by herpesvirus saimiri. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):3116–3119. doi: 10.1073/pnas.89.7.3116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Biesinger B., Trimble J. J., Desrosiers R. C., Fleckenstein B. The divergence between two oncogenic Herpesvirus saimiri strains in a genomic region related to the transforming phenotype. Virology. 1990 Jun;176(2):505–514. doi: 10.1016/0042-6822(90)90020-r. [DOI] [PubMed] [Google Scholar]
  3. Bornkamm G. W., Delius H., Fleckenstein B., Werner F. J., Mulder C. Structure of Herpesvirus saimiri genomes: arrangement of heavy and light sequences in the M genome. J Virol. 1976 Jul;19(1):154–161. doi: 10.1128/jvi.19.1.154-161.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown D. R., Deb S., Muñoz R. M., Subler M. A., Deb S. P. The tumor suppressor p53 and the oncoprotein simian virus 40 T antigen bind to overlapping domains on the MDM2 protein. Mol Cell Biol. 1993 Nov;13(11):6849–6857. doi: 10.1128/mcb.13.11.6849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cole S. R., Ashman L. K., Ey P. L. Biotinylation: an alternative to radioiodination for the identification of cell surface antigens in immunoprecipitates. Mol Immunol. 1987 Jul;24(7):699–705. doi: 10.1016/0161-5890(87)90051-4. [DOI] [PubMed] [Google Scholar]
  6. Fakharzadeh S. S., Trusko S. P., George D. L. Tumorigenic potential associated with enhanced expression of a gene that is amplified in a mouse tumor cell line. EMBO J. 1991 Jun;10(6):1565–1569. doi: 10.1002/j.1460-2075.1991.tb07676.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Geck P., Whitaker S. A., Medveczky M. M., Last T. J., Medveczky P. G. Small RNA expression from the oncogenic region of a highly oncogenic strain of herpesvirus saimiri. Virus Genes. 1994 Jan;8(1):25–34. doi: 10.1007/BF01703599. [DOI] [PubMed] [Google Scholar]
  8. Geck P., Whitaker S. A., Medveczky M. M., Medveczky P. G. Expression of collagenlike sequences by a tumor virus, herpesvirus saimiri. J Virol. 1990 Jul;64(7):3509–3515. doi: 10.1128/jvi.64.7.3509-3515.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grusby M. J., Mitchell S. C., Nabavi N., Glimcher L. H. Casein expression in cytotoxic T lymphocytes. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6897–6901. doi: 10.1073/pnas.87.17.6897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hobbs A. A., Rosen J. M. Sequence of rat alpha- and gamma-casein mRNAs: evolutionary comparison of the calcium-dependent rat casein multigene family. Nucleic Acids Res. 1982 Dec 20;10(24):8079–8098. doi: 10.1093/nar/10.24.8079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hollstein M., Sidransky D., Vogelstein B., Harris C. C. p53 mutations in human cancers. Science. 1991 Jul 5;253(5015):49–53. doi: 10.1126/science.1905840. [DOI] [PubMed] [Google Scholar]
  12. Jung J. U., Desrosiers R. C. Identification and characterization of the herpesvirus saimiri oncoprotein STP-C488. J Virol. 1991 Dec;65(12):6953–6960. doi: 10.1128/jvi.65.12.6953-6960.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lane D. P., Crawford L. V. T antigen is bound to a host protein in SV40-transformed cells. Nature. 1979 Mar 15;278(5701):261–263. doi: 10.1038/278261a0. [DOI] [PubMed] [Google Scholar]
  14. Levine A. J., Momand J., Finlay C. A. The p53 tumour suppressor gene. Nature. 1991 Jun 6;351(6326):453–456. doi: 10.1038/351453a0. [DOI] [PubMed] [Google Scholar]
  15. Liebowitz D., Kopan R., Fuchs E., Sample J., Kieff E. An Epstein-Barr virus transforming protein associates with vimentin in lymphocytes. Mol Cell Biol. 1987 Jul;7(7):2299–2308. doi: 10.1128/mcb.7.7.2299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Linzer D. I., Levine A. J. Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells. Cell. 1979 May;17(1):43–52. doi: 10.1016/0092-8674(79)90293-9. [DOI] [PubMed] [Google Scholar]
  17. Medveczky M. M., Geck P., Sullivan J. L., Serbousek D., Djeu J. Y., Medveczky P. G. IL-2 independent growth and cytotoxicity of herpesvirus saimiri-infected human CD8 cells and involvement of two open reading frame sequences of the virus. Virology. 1993 Oct;196(2):402–412. doi: 10.1006/viro.1993.1495. [DOI] [PubMed] [Google Scholar]
  18. Medveczky M. M., Geck P., Vassallo R., Medveczky P. G. Expression of the collagen-like putative oncoprotein of Herpesvirus saimiri in transformed T cells. Virus Genes. 1993 Dec;7(4):349–365. doi: 10.1007/BF01703391. [DOI] [PubMed] [Google Scholar]
  19. Medveczky M. M., Szomolanyi E., Hesselton R., DeGrand D., Geck P., Medveczky P. G. Herpesvirus saimiri strains from three DNA subgroups have different oncogenic potentials in New Zealand white rabbits. J Virol. 1989 Sep;63(9):3601–3611. doi: 10.1128/jvi.63.9.3601-3611.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Medveczky P., Szomolanyi E., Desrosiers R. C., Mulder C. Classification of herpesvirus saimiri into three groups based on extreme variation in a DNA region required for oncogenicity. J Virol. 1984 Dec;52(3):938–944. doi: 10.1128/jvi.52.3.938-944.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Melendez L. V., Daniel M. D., Hunt R. D., Garcia F. G. An apparently new herpesvirus from primary kidney cultures of the squirrel monkey (Saimiri sciureus). Lab Anim Care. 1968 Jun;18(3):374–381. [PubMed] [Google Scholar]
  22. Musashi M., Clark S. C., Sudo T., Urdal D. L., Ogawa M. Synergistic interactions between interleukin-11 and interleukin-4 in support of proliferation of primitive hematopoietic progenitors of mice. Blood. 1991 Sep 15;78(6):1448–1451. [PubMed] [Google Scholar]
  23. Sarnow P., Ho Y. S., Williams J., Levine A. J. Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells. Cell. 1982 Feb;28(2):387–394. doi: 10.1016/0092-8674(82)90356-7. [DOI] [PubMed] [Google Scholar]
  24. Shay J. W., Werbin H., Funk W. D., Wright W. E. Cellular and molecular advances in elucidating p53 function. Mutat Res. 1992 Aug;277(2):163–171. doi: 10.1016/0165-1110(92)90003-r. [DOI] [PubMed] [Google Scholar]
  25. Vogelstein B. Cancer. A deadly inheritance. Nature. 1990 Dec 20;348(6303):681–682. doi: 10.1038/348681a0. [DOI] [PubMed] [Google Scholar]
  26. Weber F., Meinl E., Drexler K., Czlonkowska A., Huber S., Fickenscher H., Müller-Fleckenstein I., Fleckenstein B., Wekerle H., Hohlfeld R. Transformation of human T-cell clones by Herpesvirus saimiri: intact antigen recognition by autonomously growing myelin basic protein-specific T cells. Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11049–11053. doi: 10.1073/pnas.90.23.11049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Werness B. A., Levine A. J., Howley P. M. Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science. 1990 Apr 6;248(4951):76–79. doi: 10.1126/science.2157286. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES