Abstract
Autographa california nuclear polyhedrosis virus (AcMNPV) contains a gene, ptp, encoding a protein tyrosine/serine phosphatase, BV-PTP. To investigate the biological function of ptp in the baculoviral replication cycle, we constructed a recombinant baculovirus, vPTPdel, in which the catalytically active site of BV-PTP was deleted. Although the vPTPdel mutant was viable in cell culture, it was partially defective in occluded virus production in SF-21 but not TN-368 cell lines. SF-21 cells infected with vPTPdel were heterogeneous in their ability to support occluded virus production. These results suggest that BV-PTP functions in a cell line-specific and possibly a cell cycle-specific fashion. The yield of occlusion bodies, infectivity (concentration of virus causing 50% mortality) and virulence (the time at which 50% of the cells died) of vPTPdel appeared to be normal in insect larvae. We identified a 35-kDa phosphoprotein as a potential target of the BV-PTP in SF-21 cells.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barford D., Flint A. J., Tonks N. K. Crystal structure of human protein tyrosine phosphatase 1B. Science. 1994 Mar 11;263(5152):1397–1404. [PubMed] [Google Scholar]
- Bliska J. B., Guan K. L., Dixon J. E., Falkow S. Tyrosine phosphate hydrolysis of host proteins by an essential Yersinia virulence determinant. Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1187–1191. doi: 10.1073/pnas.88.4.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Charbonneau H., Tonks N. K. 1002 protein phosphatases? Annu Rev Cell Biol. 1992;8:463–493. doi: 10.1146/annurev.cb.08.110192.002335. [DOI] [PubMed] [Google Scholar]
- Fischer E. H., Charbonneau H., Tonks N. K. Protein tyrosine phosphatases: a diverse family of intracellular and transmembrane enzymes. Science. 1991 Jul 26;253(5018):401–406. doi: 10.1126/science.1650499. [DOI] [PubMed] [Google Scholar]
- Guan K. L., Broyles S. S., Dixon J. E. A Tyr/Ser protein phosphatase encoded by vaccinia virus. Nature. 1991 Mar 28;350(6316):359–362. doi: 10.1038/350359a0. [DOI] [PubMed] [Google Scholar]
- Hakes D. J., Martell K. J., Zhao W. G., Massung R. F., Esposito J. J., Dixon J. E. A protein phosphatase related to the vaccinia virus VH1 is encoded in the genomes of several orthopoxviruses and a baculovirus. Proc Natl Acad Sci U S A. 1993 May 1;90(9):4017–4021. doi: 10.1073/pnas.90.9.4017. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kim D., Weaver R. F. Transcription mapping and functional analysis of the protein tyrosine/serine phosphatase (PTPase) gene of the Autographa californica nuclear polyhedrosis virus. Virology. 1993 Aug;195(2):587–595. doi: 10.1006/viro.1993.1410. [DOI] [PubMed] [Google Scholar]
- Li Y., Miller L. K. Expression and functional analysis of a baculovirus gene encoding a truncated protein kinase homolog. Virology. 1995 Jan 10;206(1):314–323. doi: 10.1016/s0042-6822(95)80047-6. [DOI] [PubMed] [Google Scholar]
- Millar J. B., McGowan C. H., Lenaers G., Jones R., Russell P. p80cdc25 mitotic inducer is the tyrosine phosphatase that activates p34cdc2 kinase in fission yeast. EMBO J. 1991 Dec;10(13):4301–4309. doi: 10.1002/j.1460-2075.1991.tb05008.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Possee R. D., Sun T. P., Howard S. C., Ayres M. D., Hill-Perkins M., Gearing K. L. Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9.4 kbp EcoRI-I and -R (polyhedrin gene) region. Virology. 1991 Nov;185(1):229–241. doi: 10.1016/0042-6822(91)90770-c. [DOI] [PubMed] [Google Scholar]
- Potts M., Sun H., Mockaitis K., Kennelly P. J., Reed D., Tonks N. K. A protein-tyrosine/serine phosphatase encoded by the genome of the cyanobacterium Nostoc commune UTEX 584. J Biol Chem. 1993 Apr 15;268(11):7632–7635. [PubMed] [Google Scholar]
- Sheng Z., Charbonneau H. The baculovirus Autographa californica encodes a protein tyrosine phosphatase. J Biol Chem. 1993 Mar 5;268(7):4728–4733. [PubMed] [Google Scholar]
- Tilakaratne N., Hardin S. E., Weaver R. F. Nucleotide sequence and transcript mapping of the HindIII F region of the Autographa californica nuclear polyhedrosis virus genome. J Gen Virol. 1991 Feb;72(Pt 2):285–291. doi: 10.1099/0022-1317-72-2-285. [DOI] [PubMed] [Google Scholar]
- Walton K. M., Dixon J. E. Protein tyrosine phosphatases. Annu Rev Biochem. 1993;62:101–120. doi: 10.1146/annurev.bi.62.070193.000533. [DOI] [PubMed] [Google Scholar]