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. 1997 Dec;78(6):598–602. doi: 10.1136/hrt.78.6.598

Interleukin 8 released after acute myocardial infarction is mainly bound to erythrocytes

R J de Winter 1, A Manten 1, Y P de Jong 1, R Adams 1, S J H van Deventer 1, K Lie 1
PMCID: PMC1892341  PMID: 9470879

Abstract

Objective—To determine whether rapid clearance of interleukin 8 (IL-8) from plasma through binding to the erythrocyte chemokine receptor may be responsible for failure to detect IL-8 consistently after acute myocardial infarction.
Design—Plasma concentrations of IL-8 were measured at frequent intervals in 43 consecutive patients. In 21 of these, erythrocyte bound IL-8 concentrations were also measured. The influence of infarct size, type of treatment, and the presence of early successful reperfusion on IL-8 release was assessed.
Results—Peak IL-8 concentrations in plasma were raised in 31 of the 43 patients (68%). Median plasma IL-8 concentrations were 16.0 pg/ml (range 2.4 to 225.0 pg/ml) six hours after the onset of chest pain. Twelve hours after the onset of symptoms, plasma IL-8 concentrations had already returned to normal in 27 patients. In contrast, in 18 of 21 patients (86%), erythrocyte bound IL-8 concentrations were raised at between 6 and 30 hours, with a median peak value of 59.8 pg/ml (range 19 to 148 pg/ml). No correlation between peak creatine kinase MB and peak IL-8 (plasma or erythrocyte bound) was observed. There was a significant difference in peak plasma IL-8 concentrations between patients who underwent direct PTCA (19.4 pg/ml) and those who received conservative treatment (9.9 pg/ml; p = 0.0206), but no correlation with the presence of early successful reperfusion.
Conclusions—IL-8 is released in plasma after acute myocardial infarction and subsequently binds to red blood cells, resulting in only a transient rise of plasma IL-8 and a more prolonged increase of erythrocyte bound IL-8.

 Keywords: acute myocardial infarction;  interleukin 8

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Figure 1  .

Figure 1  

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Scattergraph showing peak IL-8 concentrations in plasma on the X axis and peak creatine kinase MB isoenzyme (CK-MB) on the Y axis, showing the lack of correlation. Each point on the graph represents the value in one patient. Two outliers are shown on the right side of the graph with their respective CK-MB and IL-8 values. Neither erythrocyte bound IL-8 nor the sum of plasma and erythrocyte bound IL-8 was correlated with infarct size, as estimated from the peak CK-MB value (data not shown).

Figure 2  .

Figure 2  

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Mean plasma IL-8 and erythrocyte bound IL-8 from 21 patients with acute myocardial infarction. Error bars = SEM. Normal values were 10 pg/ml for plasma and 32 pg/ml for erythrocyte bound IL-8. Erythrocyte bound IL-8 concentrations peaked later than plasma concentrations, and were raised for longer.

Selected References

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