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. 1992 Jan;36(1):132–136. doi: 10.1128/aac.36.1.132

Effect of an arginine-glycine-aspartic acid-containing peptide on hematogenous candidal infections in rabbits.

S A Klotz 1, R L Smith 1, B W Stewart 1
PMCID: PMC189240  PMID: 1590678

Abstract

The adherence of Candida albicans yeast cells to the subendothelial extracellular matrix, fibronectin, laminin, and type I and IV collagen was tested. Fibronectin (10(-7) M) and a peptide, PepTite-2000 (Telios Pharmaceuticals Inc., San Diego, Calif.), containing the sequence arginine-glycine-aspartic acid (RGD) inhibited Candida adherence to these targets by greater than 90%. When C. albicans was perfused over ex vivo rabbit aortic endothelium, there was no significant difference in the amount of adherence in the presence or absence of the RGD-containing peptide. However, the RGD-containing peptide reduced the number of Candida organisms present in liver, brain, heart, and kidneys (P less than 0.05) of rabbits 4 h after intravenous inoculation of 5 x 10(7) C. albicans yeast cells. The peptide also reduced the number of macroscopic Candida abscesses in the kidneys of rabbits 72 h after intravenous inoculation of 10(7) C. albicans yeast cells (P less than 0.05). Inhibition of Candida adherence in vitro and in vivo may occur because the peptide blocks a fungal receptor that is necessary for adherence.

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Selected References

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  1. Albelda S. M., Buck C. A. Integrins and other cell adhesion molecules. FASEB J. 1990 Aug;4(11):2868–2880. [PubMed] [Google Scholar]
  2. Bouali A., Robert R., Tronchin G., Senet J. M. Binding of human fibrinogen to Candida albicans in vitro: a preliminary study. J Med Vet Mycol. 1986 Aug;24(4):345–348. doi: 10.1080/02681218680000511. [DOI] [PubMed] [Google Scholar]
  3. Bouchara J. P., Tronchin G., Annaix V., Robert R., Senet J. M. Laminin receptors on Candida albicans germ tubes. Infect Immun. 1990 Jan;58(1):48–54. doi: 10.1128/iai.58.1.48-54.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eigentler A., Schulz T. F., Larcher C., Breitwieser E. M., Myones B. L., Petzer A. L., Dierich M. P. C3bi-binding protein on Candida albicans: temperature-dependent expression and relationship to human complement receptor type 3. Infect Immun. 1989 Feb;57(2):616–622. doi: 10.1128/iai.57.2.616-622.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gospodarowicz D., Gonzalez R., Fujii D. K. Are factors originating from serum, plasma, or cultured cells involved in the growth-promoting effect of the extracellular matrix produced by cultured bovine corneal endothelial cells? J Cell Physiol. 1983 Feb;114(2):191–202. doi: 10.1002/jcp.1041140208. [DOI] [PubMed] [Google Scholar]
  6. Hart P. D., Russell E., Jr, Remington J. S. The compromised host and infection. II. Deep fungal infection. J Infect Dis. 1969 Aug;120(2):169–191. doi: 10.1093/infdis/120.2.169. [DOI] [PubMed] [Google Scholar]
  7. Heidenreich F., Dierich M. P. Candida albicans and Candida stellatoidea, in contrast to other Candida species, bind iC3b and C3d but not C3b. Infect Immun. 1985 Nov;50(2):598–600. doi: 10.1128/iai.50.2.598-600.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hostetter M. K., Lorenz J. S., Preus L., Kendrick K. E. The iC3b receptor on Candida albicans: subcellular localization and modulation of receptor expression by glucose. J Infect Dis. 1990 Apr;161(4):761–768. doi: 10.1093/infdis/161.4.761. [DOI] [PubMed] [Google Scholar]
  9. Klotz S. A., Drutz D. J., Harrison J. L., Huppert M. Adherence and penetration of vascular endothelium by Candida yeasts. Infect Immun. 1983 Oct;42(1):374–384. doi: 10.1128/iai.42.1.374-384.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klotz S. A., Maca R. D. Endothelial cell contraction increases Candida adherence to exposed extracellular matrix. Infect Immun. 1988 Sep;56(9):2495–2498. doi: 10.1128/iai.56.9.2495-2498.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klotz S. A., Smith R. L. A fibronectin receptor on Candida albicans mediates adherence of the fungus to extracellular matrix. J Infect Dis. 1991 Mar;163(3):604–610. doi: 10.1093/infdis/163.3.604. [DOI] [PubMed] [Google Scholar]
  12. Laplante C., St-Pierre S., Beaulieu A. D., Marceau F. Small fibronectin fragments induce endothelium-dependent vascular relaxations. Can J Physiol Pharmacol. 1988 Jun;66(6):745–748. doi: 10.1139/y88-118. [DOI] [PubMed] [Google Scholar]
  13. Marcantonio E. E., Hynes R. O. Antibodies to the conserved cytoplasmic domain of the integrin beta 1 subunit react with proteins in vertebrates, invertebrates, and fungi. J Cell Biol. 1988 May;106(5):1765–1772. doi: 10.1083/jcb.106.5.1765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mayer C. L., Diamond R. D., Edwards J. E., Jr Recognition of binding sites on Candida albicans by monoclonal antibodies to human leukocyte antigens. Infect Immun. 1990 Nov;58(11):3765–3769. doi: 10.1128/iai.58.11.3765-3769.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rotrosen D., Edwards J. E., Jr, Gibson T. R., Moore J. C., Cohen A. H., Green I. Adherence of Candida to cultured vascular endothelial cells: mechanisms of attachment and endothelial cell penetration. J Infect Dis. 1985 Dec;152(6):1264–1274. doi: 10.1093/infdis/152.6.1264. [DOI] [PubMed] [Google Scholar]
  16. Ruoslahti E. Integrins. J Clin Invest. 1991 Jan;87(1):1–5. doi: 10.1172/JCI114957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ruoslahti E., Pierschbacher M. D. New perspectives in cell adhesion: RGD and integrins. Science. 1987 Oct 23;238(4826):491–497. doi: 10.1126/science.2821619. [DOI] [PubMed] [Google Scholar]
  18. Sawyer R. T., Garner R. E., Hudson J. A. Arg-Gly-Asp (RGD) peptides alter hepatic killing of Candida albicans in the isolated perfused mouse liver model. Infect Immun. 1992 Jan;60(1):213–218. doi: 10.1128/iai.60.1.213-218.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sawyer R. T., Horst M. N., Garner R. E., Hudson J., Jenkins P. R., Richardson A. L. Altered hepatic clearance and killing of Candida albicans in the isolated perfused mouse liver model. Infect Immun. 1990 Sep;58(9):2869–2874. doi: 10.1128/iai.58.9.2869-2874.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Skerl K. G., Calderone R. A., Segal E., Sreevalsan T., Scheld W. M. In vitro binding of Candida albicans yeast cells to human fibronectin. Can J Microbiol. 1984 Feb;30(2):221–227. doi: 10.1139/m84-033. [DOI] [PubMed] [Google Scholar]
  21. Smith R. L., Griffin C. A. Separation of plasma fibronectin from associated hemagglutinating activity by elution from gelatin-agarose at pH 5.5. Thromb Res. 1985 Jan 1;37(1):91–101. doi: 10.1016/0049-3848(85)90036-2. [DOI] [PubMed] [Google Scholar]

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