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. 1995 Aug;69(8):5142–5146. doi: 10.1128/jvi.69.8.5142-5146.1995

Enhanced intracellular calcium concentration during poliovirus infection.

A Irurzun 1, J Arroyo 1, A Alvarez 1, L Carrasco 1
PMCID: PMC189335  PMID: 7609085

Abstract

The infection of human fibroblasts by poliovirus leads to a notable increase in the intracellular calcium concentration, [Ca2+]i, measured by microfluorimetry or by flow cytometry. [Ca2+]i increases from 2 to 3 h postinfection, and by the fifth hour there is a 5- to 10-fold increase in [Ca2+]i. At this time postinfection there is active viral protein synthesis. The modifications in [Ca2+]i are not observed in the presence of cycloheximide, guanidine, or Ro 09-0179, indicating that virus gene expression is required for the increase in [Ca2+]i. Attempts to identify the source of the intracellular Ca2+ by using different inhibitors of calcium fluxes suggest that calcium enters from the culture medium through voltage-sensitive calcium channels.

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Selected References

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