Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1995 Aug;69(8):5152–5155. doi: 10.1128/jvi.69.8.5152-5155.1995

B-lymphocyte requirement for vaccine-mediated protection from Theiler's murine encephalomyelitis virus-induced central nervous system disease.

C I Kurtz 1, X M Sun 1, R S Fujinami 1
PMCID: PMC189337  PMID: 7609086

Abstract

The role of humoral immunity in the protection of vaccinated SJL/J mice from central nervous system disease induced by the DA strain (DAV) of Theiler's murine encephalomyelitis virus was investigated in B-cell-deficient mice. Mice were depleted of B cells by treatment with a mouse monoclonal antibody specific for immunoglobulin M. DAV-vaccinated, B-cell-deficient mice failed to clear viral infection and were no longer protected from Theiler's murine encephalomyelitis virus-mediated central nervous system disease. CD4+ T cells are required in this model of protection to provide help for the development of an antiviral antibody response in the central nervous system.

Full Text

The Full Text of this article is available as a PDF (211.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cerny A., Hügin A. W., Bazin H., Sutter S., Hengartner H., Zinkernagel R. M. Anti-Listeria monocytogenes immunity in mu-suppressed mice: a comparison of treatment with conventional hyperimmune rabbit anti-mouse IgM and affinity-purified, monoclonal rat anti-mouse IgM. Med Microbiol Immunol. 1988;177(3):123–131. doi: 10.1007/BF00232892. [DOI] [PubMed] [Google Scholar]
  2. Cerny A., Sutter S., Bazin H., Hengartner H., Zinkernagel R. M. Clearance of lymphocytic choriomeningitis virus in antibody- and B-cell-deprived mice. J Virol. 1988 May;62(5):1803–1807. doi: 10.1128/jvi.62.5.1803-1807.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chesnut R. W., Colon S. M., Grey H. M. Antigen presentation by normal B cells, B cell tumors, and macrophages: functional and biochemical comparison. J Immunol. 1982 Apr;128(4):1764–1768. [PubMed] [Google Scholar]
  4. Chesnut R. W., Grey H. M. Studies on the capacity of B cells to serve as antigen-presenting cells. J Immunol. 1981 Mar;126(3):1075–1079. [PubMed] [Google Scholar]
  5. Crane M. A., Yauch R., Dal Canto M. C., Kim B. S. Effect of immunization with Theiler's virus on the course of demyelinating disease. J Neuroimmunol. 1993 Jun;45(1-2):67–73. doi: 10.1016/0165-5728(93)90165-u. [DOI] [PubMed] [Google Scholar]
  6. Fujinami R. S., Rosenthal A., Lampert P. W., Zurbriggen A., Yamada M. Survival of athymic (nu/nu) mice after Theiler's murine encephalomyelitis virus infection by passive administration of neutralizing monoclonal antibody. J Virol. 1989 May;63(5):2081–2087. doi: 10.1128/jvi.63.5.2081-2087.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gimmi C. D., Freeman G. J., Gribben J. G., Sugita K., Freedman A. S., Morimoto C., Nadler L. M. B-cell surface antigen B7 provides a costimulatory signal that induces T cells to proliferate and secrete interleukin 2. Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6575–6579. doi: 10.1073/pnas.88.15.6575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gordon J. The B lymphocyte-deprived mouse as a tool in immunobiology. J Immunol Methods. 1979;25(3):227–238. doi: 10.1016/0022-1759(79)90110-8. [DOI] [PubMed] [Google Scholar]
  9. Hayglass K. T., Naides S. J., Scott C. F., Jr, Benacerraf B., Sy M. S. T cell development in B cell-deficient mice. IV. The role of B cells as antigen-presenting cells in vivo. J Immunol. 1986 Feb 1;136(3):823–829. [PubMed] [Google Scholar]
  10. Horibata K., Harris A. W. Mouse myelomas and lymphomas in culture. Exp Cell Res. 1970 Apr;60(1):61–77. doi: 10.1016/0014-4827(70)90489-1. [DOI] [PubMed] [Google Scholar]
  11. Jordan C., Baron S., Dianzani F., Barber J., Stanton G. J. Ocular herpes simplex virus infection is diminished by depletion of B lymphocytes. J Immunol. 1983 Sep;131(3):1554–1557. [PubMed] [Google Scholar]
  12. Kurtz C. I., Sun X. M., Fujinami R. S. Protection of SJL/J mice from demyelinating disease mediated by Theiler's murine encephalomyelitis virus. Microb Pathog. 1995 Jan;18(1):11–27. doi: 10.1016/s0882-4010(05)80009-9. [DOI] [PubMed] [Google Scholar]
  13. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  14. Ledbetter J. A., Herzenberg L. A. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63–90. doi: 10.1111/j.1600-065x.1979.tb00289.x. [DOI] [PubMed] [Google Scholar]
  15. Lehrich J. R., Arnason B. G., Hochberg F. H. Demyelinative myelopathy in mice induced by the DA virus. J Neurol Sci. 1976 Oct;29(2-4):149–160. doi: 10.1016/0022-510x(76)90167-2. [DOI] [PubMed] [Google Scholar]
  16. Lipoldová M., Zajícová A., Stedra J., Holán V. Exogenous interleukin-2 abrogates differences in the proliferative responses to T cell mitogens among inbred strains of mice. Cell Immunol. 1992 Jun;142(1):177–185. doi: 10.1016/0008-8749(92)90278-w. [DOI] [PubMed] [Google Scholar]
  17. Lipton H. L., Kratochvil J., Sethi P., Dal Canto M. C. Theiler's virus antigen detected in mouse spinal cord 2 1/2 years after infection. Neurology. 1984 Aug;34(8):1117–1119. doi: 10.1212/wnl.34.8.1117. [DOI] [PubMed] [Google Scholar]
  18. Lipton H. L. Theiler's virus infection in mice: an unusual biphasic disease process leading to demyelination. Infect Immun. 1975 May;11(5):1147–1155. doi: 10.1128/iai.11.5.1147-1155.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mueller D. L., Jenkins M. K., Schwartz R. H. Clonal expansion versus functional clonal inactivation: a costimulatory signalling pathway determines the outcome of T cell antigen receptor occupancy. Annu Rev Immunol. 1989;7:445–480. doi: 10.1146/annurev.iy.07.040189.002305. [DOI] [PubMed] [Google Scholar]
  20. Rodriguez M., Kenny J. J., Thiemann R. L., Woloschak G. E. Theiler's virus-induced demyelination in mice immunosuppressed with anti-IgM and in mice expressing the xid gene. Microb Pathog. 1990 Jan;8(1):23–35. doi: 10.1016/0882-4010(90)90005-b. [DOI] [PubMed] [Google Scholar]
  21. Rodriguez M., Lucchinetti C. F., Clark R. J., Yakash T. L., Markowitz H., Lennon V. A. Immunoglobulins and complement in demyelination induced in mice by Theiler's virus. J Immunol. 1988 Feb 1;140(3):800–806. [PubMed] [Google Scholar]
  22. Ron Y., De Baetselier P., Gordon J., Feldman M., Segal S. Defective induction of antigen-reactive proliferating T cells in B cell-deprived mice. Eur J Immunol. 1981 Dec;11(12):964–968. doi: 10.1002/eji.1830111203. [DOI] [PubMed] [Google Scholar]
  23. Rosenstreich D. L., Mizel S. B. The participation of macrophages and macrophage cell lines in the activation of T lymphocytes by mitogens. Immunol Rev. 1978;40:102–135. doi: 10.1111/j.1600-065x.1978.tb00403.x. [DOI] [PubMed] [Google Scholar]
  24. Rossi C. P., Cash E., Aubert C., Coutinho A. Role of the humoral immune response in resistance to Theiler's virus infection. J Virol. 1991 Jul;65(7):3895–3899. doi: 10.1128/jvi.65.7.3895-3899.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sacks D. L., Scott P. A., Asofsky R., Sher F. A. Cutaneous leishmaniasis in anti-IgM-treated mice: enhanced resistance due to functional depletion of a B cell-dependent T cell involved in the suppressor pathway. J Immunol. 1984 Apr;132(4):2072–2077. [PubMed] [Google Scholar]
  26. Stall A. M., Loken M. R. Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies. J Immunol. 1984 Feb;132(2):787–795. [PubMed] [Google Scholar]
  27. Sy M. S., Hayglass K. T., Benacerraf B. T cell development in B cell-deficient mice. II. Serological characterization of suppressor T cell factors (TsF1) produced in normal mice and in mice treated chronically with rabbit anti-mouse IgM antibodies. J Exp Med. 1985 Jun 1;161(6):1402–1413. doi: 10.1084/jem.161.6.1402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wada Y., Fujinami R. S. Viral infection and dissemination through the olfactory pathway and the limbic system by Theiler's virus. Am J Pathol. 1993 Jul;143(1):221–229. [PMC free article] [PubMed] [Google Scholar]
  29. Yamada M., Zurbriggen A., Fujinami R. S. Pathogenesis of Theiler's murine encephalomyelitis virus. Adv Virus Res. 1991;39:291–320. doi: 10.1016/S0065-3527(08)60798-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zurbriggen A., Thomas C., Yamada M., Roos R. P., Fujinami R. S. Direct evidence of a role for amino acid 101 of VP-1 in central nervous system disease in Theiler's murine encephalomyelitis virus infection. J Virol. 1991 Apr;65(4):1929–1937. doi: 10.1128/jvi.65.4.1929-1937.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES