Figure 6.—

Effects of MID mutation and MTD1 knockdown on expression of some gamete-specific genes. (A) RT–PCR of various minus-specific genes in wild type, mid-2, and MTD1 RNAi minus progeny G2 and P3. CRY1 is used as an internal control. “No temp,” negative control in PCR with no addition of DNA template. (B) RT–PCR to detect expression levels of NSG6 and NSG17 in different MTD1 RNAi progeny. “No temp,” negative control in PCR with no addition of DNA template. “Genomic,” positive control in PCR using genomic DNA as templates.