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. 1995 Sep;69(9):5898–5903. doi: 10.1128/jvi.69.9.5898-5903.1995

Tumor necrosis factor expression during mouse hepatitis virus-induced demyelinating encephalomyelitis.

S A Stohlman 1, D R Hinton 1, D Cua 1, E Dimacali 1, J Sensintaffar 1, F M Hofman 1, S M Tahara 1, Q Yao 1
PMCID: PMC189470  PMID: 7637037

Abstract

Neutralizing anti-tumor necrosis factor alpha (TNF-alpha) antibody treatment of mice infected with the neurotropic JHMV strain of mouse hepatitis virus showed no reduction of either virus-induced encephalomyelitis or central nervous system demyelination. TNF-alpha-positive cells were present in the central nervous system during infection; however, TNF-alpha could not be colocalized with JHMV-infected cells. In vitro, TNF-alpha mRNA rapidly accumulated following JHMV infection; however, no TNF-alpha was secreted because of inhibition of translation. Both live and UV-inactivated virus inhibited TNF-alpha secretion induced by lipopolysaccharide. These data show that TNF-alpha is not secreted from infected cells and indicate that if contributes to either JHMV-induced acute encephalomyelitis nor primary demyelination.

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Selected References

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