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. 1995 Sep;69(9):5908–5911. doi: 10.1128/jvi.69.9.5908-5911.1995

Efficient magnesium-dependent human immunodeficiency virus type 1 integrase activity.

A Engelman 1, R Craigie 1
PMCID: PMC189473  PMID: 7637039

Abstract

The integrase protein from human immunodeficiency virus type 1 (HIV-1) has generally been reported to require Mn2+ for efficient in vitro activity. We have reexamined the divalent metal ion requirements of HIV-1 integrase and find that the protein is capable of promoting efficient 3' processing and DNA strand transfer with either Mn2+ or Mg2+. The metal ion preference depended upon the reaction conditions. HIV-1 integrase displayed significantly less nonspecific nuclease activity in reaction mixtures containing Mg2+ than it did under the previously described reaction conditions with mixtures containing Mn2+.

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Selected References

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