Figure 6.

Specificity of the NF-κB–binding site. EMSAs were performed using nuclear protein from HUVECs and biotin-labeled probes (WT [wild-type] oligo, biotin or Mut oligo, biotin) as described in Figure 5. Symbols above each lane (+/-) denote the presence or absence of various reagents. For competition experiments (lanes 4 and 6), a 50-fold excess of unlabeled oligo (WT oligo or Mut oligo) was used. Data are representative of at least 3 independent experiments.