Figure 6.

Expression of GATA-1 in human T_N,T_CM, and T_EM cell subsets induces expression of Th2-associated chemokine receptors. Human T_N, T_CM, and T_EM cells were purified, activated, and transduced with HDV alone, HDV.GATA-1, or HDV.GATA-3 as described in caption for Figure 5. Following expansion, unsorted cells were stained for chemotactic receptors as indicated or an isotype control in conjunction with an anti-mCD24 antibody to gate on transduced populations. The percentage of cells positive for the appropriate chemokine receptor is shown, with the gray, tinted peak representing isotype control staining. Results are representative of 3 experiments using T-cell subsets from distinct adult donors.