Table 1.
Surface phenotype of liver and bone marrow SP and non-SP cells
|
Liver, %
|
Bone marrow, %
|
||||
|---|---|---|---|---|---|
| SP tip | SP shoulder | Non-SP | SP | Non-SP | |
| Sca-1+ | 60 | 30 | 15 | 90 | 5 |
| CD45+ | 3 | 64 | 32 | 99 | 95 |
| c-kit+* | < 1 | 1 | 40 | 95 | 12 |
| Lin+† | 2 | 4 | 10 | 10 | 85 |
| CD31+ | 10 | 43 | 50 | 95 | 50 |
| CD29+ | 99 | 90 | 70 | 95 | 25 |
| CD49f+ | 90 | 20 | 50 | 90 | 5 |
FACS-based analysis of surface immunostaining was used to determine the average percentage of cells expressing a specified surface marker in each cell population analyzed.
*
Enzyme digestion diminishes the intensity of c-kit immunostaining as detailed in “CD45+ SP tip cells possess phenotypic characteristics distinct from bone marrow HSCs.”
†
Lin indicates CD3, B220, CD11b, GR-1, and Ter-119.