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. 1995 Oct;69(10):6605–6608. doi: 10.1128/jvi.69.10.6605-6608.1995

Rescue of vaccinia virus lacking the E3L gene by mutants of E3L.

H W Chang 1, L H Uribe 1, B L Jacobs 1
PMCID: PMC189568  PMID: 7666567

Abstract

Vaccinia virus with the E3L gene deleted was able to replicate in RK-13 but not HeLa cells. This host range phenotype could be complemented by an E3L gene expressed transiently from a plasmid. Analysis of mutants of E3L indicates that the ability to complement deletion of E3L correlates with the ability of mutated proteins to bind double-stranded RNA but not with their ability to migrate to the nucleus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baier L. J., Shors T., Shors S. T., Jacobs B. L. The mouse antiphosphotyrosine immunoreactive kinase, TIK, is indistinguishable from the double-stranded RNA-dependent, interferon-induced protein kinase, PKR. Nucleic Acids Res. 1993 Oct 11;21(20):4830–4835. doi: 10.1093/nar/21.20.4830. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beattie E., Denzler K. L., Tartaglia J., Perkus M. E., Paoletti E., Jacobs B. L. Reversal of the interferon-sensitive phenotype of a vaccinia virus lacking E3L by expression of the reovirus S4 gene. J Virol. 1995 Jan;69(1):499–505. doi: 10.1128/jvi.69.1.499-505.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beattie E., Tartaglia J., Paoletti E. Vaccinia virus-encoded eIF-2 alpha homolog abrogates the antiviral effect of interferon. Virology. 1991 Jul;183(1):419–422. doi: 10.1016/0042-6822(91)90158-8. [DOI] [PubMed] [Google Scholar]
  4. Carroll K., Elroy-Stein O., Moss B., Jagus R. Recombinant vaccinia virus K3L gene product prevents activation of double-stranded RNA-dependent, initiation factor 2 alpha-specific protein kinase. J Biol Chem. 1993 Jun 15;268(17):12837–12842. [PubMed] [Google Scholar]
  5. Chang H. W., Jacobs B. L. Identification of a conserved motif that is necessary for binding of the vaccinia virus E3L gene products to double-stranded RNA. Virology. 1993 Jun;194(2):537–547. doi: 10.1006/viro.1993.1292. [DOI] [PubMed] [Google Scholar]
  6. Chang H. W., Watson J. C., Jacobs B. L. The E3L gene of vaccinia virus encodes an inhibitor of the interferon-induced, double-stranded RNA-dependent protein kinase. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4825–4829. doi: 10.1073/pnas.89.11.4825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davies M. V., Chang H. W., Jacobs B. L., Kaufman R. J. The E3L and K3L vaccinia virus gene products stimulate translation through inhibition of the double-stranded RNA-dependent protein kinase by different mechanisms. J Virol. 1993 Mar;67(3):1688–1692. doi: 10.1128/jvi.67.3.1688-1692.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Davies M. V., Elroy-Stein O., Jagus R., Moss B., Kaufman R. J. The vaccinia virus K3L gene product potentiates translation by inhibiting double-stranded-RNA-activated protein kinase and phosphorylation of the alpha subunit of eukaryotic initiation factor 2. J Virol. 1992 Apr;66(4):1943–1950. doi: 10.1128/jvi.66.4.1943-1950.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goebel S. J., Johnson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. The complete DNA sequence of vaccinia virus. Virology. 1990 Nov;179(1):247-66, 517-63. doi: 10.1016/0042-6822(90)90294-2. [DOI] [PubMed] [Google Scholar]
  10. Huismans H., Joklik W. K. Reovirus-coded polypeptides in infected cells: isolation of two native monomeric polypeptides with affinity for single-stranded and double-stranded RNA, respectively. Virology. 1976 Apr;70(2):411–424. doi: 10.1016/0042-6822(76)90282-8. [DOI] [PubMed] [Google Scholar]
  11. Kaufman R. J., Davies M. V., Pathak V. K., Hershey J. W. The phosphorylation state of eucaryotic initiation factor 2 alters translational efficiency of specific mRNAs. Mol Cell Biol. 1989 Mar;9(3):946–958. doi: 10.1128/mcb.9.3.946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Langland J. O., Pettiford S., Jiang B., Jacobs B. L. Products of the porcine group C rotavirus NSP3 gene bind specifically to double-stranded RNA and inhibit activation of the interferon-induced protein kinase PKR. J Virol. 1994 Jun;68(6):3821–3829. doi: 10.1128/jvi.68.6.3821-3829.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Park H., Davies M. V., Langland J. O., Chang H. W., Nam Y. S., Tartaglia J., Paoletti E., Jacobs B. L., Kaufman R. J., Venkatesan S. TAR RNA-binding protein is an inhibitor of the interferon-induced protein kinase PKR. Proc Natl Acad Sci U S A. 1994 May 24;91(11):4713–4717. doi: 10.1073/pnas.91.11.4713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shchelkunov S. N., Blinov V. M., Sandakhchiev L. S. Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms. FEBS Lett. 1993 Mar 15;319(1-2):80–83. doi: 10.1016/0014-5793(93)80041-r. [DOI] [PubMed] [Google Scholar]
  15. Watson J. C., Chang H. W., Jacobs B. L. Characterization of a vaccinia virus-encoded double-stranded RNA-binding protein that may be involved in inhibition of the double-stranded RNA-dependent protein kinase. Virology. 1991 Nov;185(1):206–216. doi: 10.1016/0042-6822(91)90768-7. [DOI] [PubMed] [Google Scholar]
  16. Whitaker-Dowling P., Youngner J. S. Vaccinia rescue of VSV from interferon-induced resistance: reversal of translation block and inhibition of protein kinase activity. Virology. 1983 Nov;131(1):128–136. doi: 10.1016/0042-6822(83)90539-1. [DOI] [PubMed] [Google Scholar]
  17. Whitaker-Dowling P., Youngner J. S. Vaccinia-mediated rescue of encephalomyocarditis virus from the inhibitory effects of interferon. Virology. 1986 Jul 15;152(1):50–57. doi: 10.1016/0042-6822(86)90370-3. [DOI] [PubMed] [Google Scholar]
  18. Youngner J. S., Thacore H. R., Kelly M. E. Sensitivity of ribonucleic acid and deoxyribonucleic acid viruses to different species of interferon in cell cultures. J Virol. 1972 Aug;10(2):171–178. doi: 10.1128/jvi.10.2.171-178.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yuwen H., Cox J. H., Yewdell J. W., Bennink J. R., Moss B. Nuclear localization of a double-stranded RNA-binding protein encoded by the vaccinia virus E3L gene. Virology. 1993 Aug;195(2):732–744. doi: 10.1006/viro.1993.1424. [DOI] [PubMed] [Google Scholar]

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