Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1995 Nov;69(11):7278–7281. doi: 10.1128/jvi.69.11.7278-7281.1995

A point mutation in the VP4 coding sequence of coxsackievirus B4 influences virulence.

A I Ramsingh 1, D N Collins 1
PMCID: PMC189653  PMID: 7474153

Abstract

In analyzing the molecular determinants of virulence of coxsackievirus B4, chimeric viruses were constructed from avirulent and virulent viruses. The vCB424 recombinant contained a single nucleotide substitution on an avirulent genetic background, resulting in replacement of Ser-16 of VP4 with Arg-16. Mice infected with vCB424 displayed an intermediate phenotype.

Full Text

The Full Text of this article is available as a PDF (756.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barger M. T., Craighead J. E. Immunomodulation of encephalomyocarditis virus-induced disease in A/J mice. J Virol. 1991 May;65(5):2676–2681. doi: 10.1128/jvi.65.5.2676-2681.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Caggana M., Chan P., Ramsingh A. Identification of a single amino acid residue in the capsid protein VP1 of coxsackievirus B4 that determines the virulent phenotype. J Virol. 1993 Aug;67(8):4797–4803. doi: 10.1128/jvi.67.8.4797-4803.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chapman N. M., Tu Z., Tracy S., Gauntt C. J. An infectious cDNA copy of the genome of a non-cardiovirulent coxsackievirus B3 strain: its complete sequence analysis and comparison to the genomes of cardiovirulent coxsackieviruses. Arch Virol. 1994;135(1-2):115–130. doi: 10.1007/BF01309769. [DOI] [PubMed] [Google Scholar]
  4. Couderc T., Guédo N., Calvez V., Pelletier I., Hogle J., Colbère-Garapin F., Blondel B. Substitutions in the capsids of poliovirus mutants selected in human neuroblastoma cells confer on the Mahoney type 1 strain a phenotype neurovirulent in mice. J Virol. 1994 Dec;68(12):8386–8391. doi: 10.1128/jvi.68.12.8386-8391.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Couderc T., Hogle J., Le Blay H., Horaud F., Blondel B. Molecular characterization of mouse-virulent poliovirus type 1 Mahoney mutants: involvement of residues of polypeptides VP1 and VP2 located on the inner surface of the capsid protein shell. J Virol. 1993 Jul;67(7):3808–3817. doi: 10.1128/jvi.67.7.3808-3817.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grist N. R., Bell E. J., Assaad F. Enteroviruses in human disease. Prog Med Virol. 1978;24:114–157. [PubMed] [Google Scholar]
  7. Hellen C. U., Wimmer E. Maturation of poliovirus capsid proteins. Virology. 1992 Apr;187(2):391–397. doi: 10.1016/0042-6822(92)90440-z. [DOI] [PubMed] [Google Scholar]
  8. Jenkins O., Booth J. D., Minor P. D., Almond J. W. The complete nucleotide sequence of coxsackievirus B4 and its comparison to other members of the Picornaviridae. J Gen Virol. 1987 Jul;68(Pt 7):1835–1848. doi: 10.1099/0022-1317-68-7-1835. [DOI] [PubMed] [Google Scholar]
  9. Kawamura N., Kohara M., Abe S., Komatsu T., Tago K., Arita M., Nomoto A. Determinants in the 5' noncoding region of poliovirus Sabin 1 RNA that influence the attenuation phenotype. J Virol. 1989 Mar;63(3):1302–1309. doi: 10.1128/jvi.63.3.1302-1309.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lu H. H., Yang C. F., Murdin A. D., Klein M. H., Harber J. J., Kew O. M., Wimmer E. Mouse neurovirulence determinants of poliovirus type 1 strain LS-a map to the coding regions of capsid protein VP1 and proteinase 2Apro. J Virol. 1994 Nov;68(11):7507–7515. doi: 10.1128/jvi.68.11.7507-7515.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Macadam A. J., Pollard S. R., Ferguson G., Dunn G., Skuce R., Almond J. W., Minor P. D. The 5' noncoding region of the type 2 poliovirus vaccine strain contains determinants of attenuation and temperature sensitivity. Virology. 1991 Apr;181(2):451–458. doi: 10.1016/0042-6822(91)90877-e. [DOI] [PubMed] [Google Scholar]
  12. Moscufo N., Yafal A. G., Rogove A., Hogle J., Chow M. A mutation in VP4 defines a new step in the late stages of cell entry by poliovirus. J Virol. 1993 Aug;67(8):5075–5078. doi: 10.1128/jvi.67.8.5075-5078.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RAABO E., TERKILDSEN T. C. On the enzymatic determination of blood glucose. Scand J Clin Lab Invest. 1960;12(4):402–407. doi: 10.3109/00365516009065404. [DOI] [PubMed] [Google Scholar]
  14. Ramsingh A., Araki H., Bryant S., Hixson A. Identification of candidate sequences that determine virulence in Coxsackievirus B4. Virus Res. 1992 May;23(3):281–292. doi: 10.1016/0168-1702(92)90114-o. [DOI] [PubMed] [Google Scholar]
  15. Ramsingh A., Hixson A., Duceman B., Slack J. Evidence suggesting that virulence maps to the P1 region of the coxsackievirus B4 genome. J Virol. 1990 Jun;64(6):3078–3081. doi: 10.1128/jvi.64.6.3078-3081.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ramsingh A., Slack J., Silkworth J., Hixson A. Severity of disease induced by a pancreatropic Coxsackie B4 virus correlates with the H-2Kq locus of the major histocompatibility complex. Virus Res. 1989 Dec;14(4):347–358. doi: 10.1016/0168-1702(89)90027-0. [DOI] [PubMed] [Google Scholar]
  17. Ren R. B., Moss E. G., Racaniello V. R. Identification of two determinants that attenuate vaccine-related type 2 poliovirus. J Virol. 1991 Mar;65(3):1377–1382. doi: 10.1128/jvi.65.3.1377-1382.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tatem J. M., Weeks-Levy C., Georgiu A., DiMichele S. J., Gorgacz E. J., Racaniello V. R., Cano F. R., Mento S. J. A mutation present in the amino terminus of Sabin 3 poliovirus VP1 protein is attenuating. J Virol. 1992 May;66(5):3194–3197. doi: 10.1128/jvi.66.5.3194-3197.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Westrop G. D., Wareham K. A., Evans D. M., Dunn G., Minor P. D., Magrath D. I., Taffs F., Marsden S., Skinner M. A., Schild G. C. Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine. J Virol. 1989 Mar;63(3):1338–1344. doi: 10.1128/jvi.63.3.1338-1344.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zhang H. Y., Yousef G. E., Cunningham L., Blake N. W., OuYang X., Bayston T. A., Kandolf R., Archard L. C. Attenuation of a reactivated cardiovirulent coxsackievirus B3: The 5'-nontranslated region does not contain major attenuation determinants. J Med Virol. 1993 Oct;41(2):129–137. doi: 10.1002/jmv.1890410208. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES