Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1995 Nov;69(11):7328–7333. doi: 10.1128/jvi.69.11.7328-7333.1995

Detection of feline immunodeficiency virus in semen from seropositive domestic cats (Felis catus).

H L Jordan 1, J Howard 1, W A Tompkins 1, S Kennedy-Stoskopf 1
PMCID: PMC189664  PMID: 7474164

Abstract

Electroejaculates from experimentally infected domestic cats were evaluated for the presence of feline immunodeficiency virus (FIV). Virus was isolated from cell-free seminal plasma and seminal cells by cocultivation with a feline interleukin-2-dependent CD4+ T-cell line, in which productive infection was demonstrated by syncytium formation and FIV gag p26 antigen secretion. In addition, an 868-bp segment of the FIV gag provirus gene was identified in cocultured cells by PCR and Southern analysis. A 582-bp fragment of the FIV gag provirus genome was detected by nested PCR and Southern analysis in nonfractionated seminal cells and in sperm purified by a swim-up procedure. This is the first report describing the detection of replication-competent FIV in cell-free and cell-associated forms in domestic cat semen.

Full Text

The Full Text of this article is available as a PDF (899.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baccetti B., Benedetto A., Burrini A. G., Collodel G., Ceccarini E. C., Crisà N., Di Caro A., Estenoz M., Garbuglia A. R., Massacesi A. HIV-particles in spermatozoa of patients with AIDS and their transfer into the oocyte. J Cell Biol. 1994 Nov;127(4):903–914. doi: 10.1083/jcb.127.4.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barone M. A., Wildt D. E., Byers A. P., Roelke M. E., Glass C. M., Howard J. G. Gonadotrophin dose and timing of anaesthesia for laparoscopic artificial insemination in the puma (Felis concolor). J Reprod Fertil. 1994 May;101(1):103–108. doi: 10.1530/jrf.0.1010103. [DOI] [PubMed] [Google Scholar]
  3. Bendinelli M., Pistello M., Lombardi S., Poli A., Garzelli C., Matteucci D., Ceccherini-Nelli L., Malvaldi G., Tozzini F. Feline immunodeficiency virus: an interesting model for AIDS studies and an important cat pathogen. Clin Microbiol Rev. 1995 Jan;8(1):87–112. doi: 10.1128/cmr.8.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown E. W., Yuhki N., Packer C., O'Brien S. J. A lion lentivirus related to feline immunodeficiency virus: epidemiologic and phylogenetic aspects. J Virol. 1994 Sep;68(9):5953–5968. doi: 10.1128/jvi.68.9.5953-5968.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Danave I. R., Tiffany-Castiglioni E., Zenger E., Barhoumi R., Burghardt R. C., Collisson E. W. Feline immunodeficiency virus decreases cell-cell communication and mitochondrial membrane potential. J Virol. 1994 Oct;68(10):6745–6750. doi: 10.1128/jvi.68.10.6745-6750.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. English R. V., Johnson C. M., Gebhard D. H., Tompkins M. B. In vivo lymphocyte tropism of feline immunodeficiency virus. J Virol. 1993 Sep;67(9):5175–5186. doi: 10.1128/jvi.67.9.5175-5186.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. English R. V., Nelson P., Johnson C. M., Nasisse M., Tompkins W. A., Tompkins M. B. Development of clinical disease in cats experimentally infected with feline immunodeficiency virus. J Infect Dis. 1994 Sep;170(3):543–552. doi: 10.1093/infdis/170.3.543. [DOI] [PubMed] [Google Scholar]
  8. Hamed K. A., Winters M. A., Holodniy M., Katzenstein D. A., Merigan T. C. Detection of human immunodeficiency virus type 1 in semen: effects of disease stage and nucleoside therapy. J Infect Dis. 1993 Apr;167(4):798–802. doi: 10.1093/infdis/167.4.798. [DOI] [PubMed] [Google Scholar]
  9. Hosie M. J., Robertson C., Jarrett O. Prevalence of feline leukaemia virus and antibodies to feline immunodeficiency virus in cats in the United Kingdom. Vet Rec. 1989 Sep 9;125(11):293–297. doi: 10.1136/vr.125.11.293. [DOI] [PubMed] [Google Scholar]
  10. Howard J. G., Barone M. A., Donoghue A. M., Wildt D. E. The effect of pre-ovulatory anaesthesia on ovulation in laparoscopically inseminated domestic cats. J Reprod Fertil. 1992 Sep;96(1):175–186. doi: 10.1530/jrf.0.0960175. [DOI] [PubMed] [Google Scholar]
  11. Howard J. G., Brown J. L., Bush M., Wildt D. E. Teratospermic and normospermic domestic cats: ejaculate traits, pituitary-gonadal hormones, and improvement of spermatozoal motility and morphology after swim-up processing. J Androl. 1990 May-Jun;11(3):204–215. [PubMed] [Google Scholar]
  12. Ishida T., Washizu T., Toriyabe K., Motoyoshi S., Tomoda I., Pedersen N. C. Feline immunodeficiency virus infection in cats of Japan. J Am Vet Med Assoc. 1989 Jan 15;194(2):221–225. [PubMed] [Google Scholar]
  13. Krieger J. N., Coombs R. W., Collier A. C., Ross S. O., Chaloupka K., Cummings D. K., Murphy V. L., Corey L. Recovery of human immunodeficiency virus type 1 from semen: minimal impact of stage of infection and current antiviral chemotherapy. J Infect Dis. 1991 Feb;163(2):386–388. doi: 10.1093/infdis/163.2.386. [DOI] [PubMed] [Google Scholar]
  14. Kuller L., Benveniste R. E., Watanabe R., Tsai C. C., Morton W. R. Transmission of SIVMne from female to male Macaca nemestrina. J Med Primatol. 1992 Aug;21(6):299–307. [PubMed] [Google Scholar]
  15. Levy J. A. The transmission of AIDS: the case of the infected cell. JAMA. 1988 May 27;259(20):3037–3038. [PubMed] [Google Scholar]
  16. Matteucci D., Baldinotti F., Mazzetti P., Pistello M., Bandecchi P., Ghilarducci R., Poli A., Tozzini F., Bendinelli M. Detection of feline immunodeficiency virus in saliva and plasma by cultivation and polymerase chain reaction. J Clin Microbiol. 1993 Mar;31(3):494–501. doi: 10.1128/jcm.31.3.494-501.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mermin J. H., Holodniy M., Katzenstein D. A., Merigan T. C. Detection of human immunodeficiency virus DNA and RNA in semen by the polymerase chain reaction. J Infect Dis. 1991 Oct;164(4):769–772. doi: 10.1093/infdis/164.4.769. [DOI] [PubMed] [Google Scholar]
  18. Miller C. J., Alexander N. J., Sutjipto S., Lackner A. A., Gettie A., Hendrickx A. G., Lowenstine L. J., Jennings M., Marx P. A. Genital mucosal transmission of simian immunodeficiency virus: animal model for heterosexual transmission of human immunodeficiency virus. J Virol. 1989 Oct;63(10):4277–4284. doi: 10.1128/jvi.63.10.4277-4284.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moench T. R., Whaley K. J., Mandrell T. D., Bishop B. D., Witt C. J., Cone R. A. The cat/feline immunodeficiency virus model for transmucosal transmission of AIDS: nonoxynol-9 contraceptive jelly blocks transmission by an infected cell inoculum. AIDS. 1993 Jun;7(6):797–802. doi: 10.1097/00002030-199306000-00006. [DOI] [PubMed] [Google Scholar]
  20. Nash J. W., Hanson L. A., St Cyr Coats K. Bovine immunodeficiency virus in stud bull semen. Am J Vet Res. 1995 Jun;56(6):760–763. [PubMed] [Google Scholar]
  21. Nuovo G. J., Becker J., Simsir A., Margiotta M., Khalife G., Shevchuk M. HIV-1 nucleic acids localize to the spermatogonia and their progeny. A study by polymerase chain reaction in situ hybridization. Am J Pathol. 1994 Jun;144(6):1142–1148. [PMC free article] [PubMed] [Google Scholar]
  22. O'Neil L. L., Burkhard M. J., Diehl L. J., Hoover E. A. Vertical transmission of feline immunodeficiency virus. AIDS Res Hum Retroviruses. 1995 Jan;11(1):171–182. doi: 10.1089/aid.1995.11.171. [DOI] [PubMed] [Google Scholar]
  23. Olmsted R. A., Langley R., Roelke M. E., Goeken R. M., Adger-Johnson D., Goff J. P., Albert J. P., Packer C., Laurenson M. K., Caro T. M. Worldwide prevalence of lentivirus infection in wild feline species: epidemiologic and phylogenetic aspects. J Virol. 1992 Oct;66(10):6008–6018. doi: 10.1128/jvi.66.10.6008-6018.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pedersen N. C., Yamamoto J. K., Ishida T., Hansen H. Feline immunodeficiency virus infection. Vet Immunol Immunopathol. 1989 May;21(1):111–129. doi: 10.1016/0165-2427(89)90134-7. [DOI] [PubMed] [Google Scholar]
  25. Phillips-Conroy J. E., Jolly C. J., Petros B., Allan J. S., Desrosiers R. C. Sexual transmission of SIVagm in wild grivet monkeys. J Med Primatol. 1994 Jan;23(1):1–7. doi: 10.1111/j.1600-0684.1994.tb00088.x. [DOI] [PubMed] [Google Scholar]
  26. Poli A., Abramo F., Cavicchio P., Bandecchi P., Ghelardi E., Pistello M. Lentivirus infection in an African lion: a clinical, pathologic and virologic study. J Wildl Dis. 1995 Jan;31(1):70–74. doi: 10.7589/0090-3558-31.1.70. [DOI] [PubMed] [Google Scholar]
  27. Portis J. L., McAtee F. J., Hayes S. F. Horizontal transmission of murine retroviruses. J Virol. 1987 Apr;61(4):1037–1044. doi: 10.1128/jvi.61.4.1037-1044.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Pudney J., Anderson D. Orchitis and human immunodeficiency virus type 1 infected cells in reproductive tissues from men with the acquired immune deficiency syndrome. Am J Pathol. 1991 Jul;139(1):149–160. [PMC free article] [PubMed] [Google Scholar]
  29. Sellon R. K., Jordan H. L., Kennedy-Stoskopf S., Tompkins M. B., Tompkins W. A. Feline immunodeficiency virus can be experimentally transmitted via milk during acute maternal infection. J Virol. 1994 May;68(5):3380–3385. doi: 10.1128/jvi.68.5.3380-3385.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tashjian R. J. Transmission and clinical evaluation of an equine infectious anemia herd and their offspring over a 13-year period. J Am Vet Med Assoc. 1984 Feb 1;184(3):282–288. [PubMed] [Google Scholar]
  31. Tompkins M. B., Nelson P. D., English R. V., Novotney C. Early events in the immunopathogenesis of feline retrovirus infections. J Am Vet Med Assoc. 1991 Nov 15;199(10):1311–1315. [PubMed] [Google Scholar]
  32. Torten M., Franchini M., Barlough J. E., George J. W., Mozes E., Lutz H., Pedersen N. C. Progressive immune dysfunction in cats experimentally infected with feline immunodeficiency virus. J Virol. 1991 May;65(5):2225–2230. doi: 10.1128/jvi.65.5.2225-2230.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Van Voorhis B. J., Martinez A., Mayer K., Anderson D. J. Detection of human immunodeficiency virus type 1 in semen from seropositive men using culture and polymerase chain reaction deoxyribonucleic acid amplification techniques. Fertil Steril. 1991 Mar;55(3):588–594. [PubMed] [Google Scholar]
  34. Wolff H., Anderson D. J. Immunohistologic characterization and quantitation of leukocyte subpopulations in human semen. Fertil Steril. 1988 Mar;49(3):497–504. [PubMed] [Google Scholar]
  35. Yamamoto J. K., Hansen H., Ho E. W., Morishita T. Y., Okuda T., Sawa T. R., Nakamura R. M., Pedersen N. C. Epidemiologic and clinical aspects of feline immunodeficiency virus infection in cats from the continental United States and Canada and possible mode of transmission. J Am Vet Med Assoc. 1989 Jan 15;194(2):213–220. [PubMed] [Google Scholar]
  36. Yamamoto J. K., Sparger E., Ho E. W., Andersen P. R., O'Connor T. P., Mandell C. P., Lowenstine L., Munn R., Pedersen N. C. Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats. Am J Vet Res. 1988 Aug;49(8):1246–1258. [PubMed] [Google Scholar]
  37. Zhang H., Zhang Y., Spicer T. P., Abbott L. Z., Abbott M., Poiesz B. J. Reverse transcription takes place within extracellular HIV-1 virions: potential biological significance. AIDS Res Hum Retroviruses. 1993 Dec;9(12):1287–1296. doi: 10.1089/aid.1993.9.1287. [DOI] [PubMed] [Google Scholar]
  38. da Silva M., Shevchuk M. M., Cronin W. J., Armenakas N. A., Tannenbaum M., Fracchia J. A., Ioachim H. L. Detection of HIV-related protein in testes and prostates of patients with AIDS. Am J Clin Pathol. 1990 Feb;93(2):196–201. doi: 10.1093/ajcp/93.2.196. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES