Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1995 Dec;69(12):7982–7990. doi: 10.1128/jvi.69.12.7982-7990.1995

Genotypic selection of simian immunodeficiency virus in macaque infants infected transplacentally.

A M Amedee 1, N Lacour 1, J L Gierman 1, L N Martin 1, J E Clements 1, R Bohm Jr 1, R M Harrison 1, M Murphey-Corb 1
PMCID: PMC189743  PMID: 7494311

Abstract

To understand viral and host factors that contribute to transplacental transmission of human immunodeficiency virus, we developed an animal model using pregnant female macaques infected with simian immunodeficiency virus (SIV). Pregnant females were inoculated intravenously during midgestation with either a well-characterized primary isolate of SIV (SIV/DeltaB670) or a combination of SIV/DeltaB670 and the macrophage-tropic molecular clone SIV/17E-Fr. The viral genetic diversity in five infected female macaques and their in utero-infected infants was analyzed. All of the mothers harbored a genetically diverse virus population at parturition, whereas a single genotype from the maternal quasispecies was identified in the infants at birth. Only one of two variants was found in the infants: SIV/17E-Fr (two cases) or a genotype contained within the SIV/DeltaB670 quasispecies (three cases). The macrophage-tropic properties of both transmitted genotypes were suggested by productive replication in primary rhesus macrophage cultures in vitro and the clonal presence in central nervous system tissue of infected monkeys with encephalitis. These observations provide compelling evidence for both genotypic and phenotypic selection in transplacental transmission of SIV and suggest a critical role for macrophages in fetal infection in utero.

Full Text

The Full Text of this article is available as a PDF (277.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson M. G., Hauer D., Sharma D. P., Joag S. V., Narayan O., Zink M. C., Clements J. E. Analysis of envelope changes acquired by SIVmac239 during neuroadaption in rhesus macaques. Virology. 1993 Aug;195(2):616–626. doi: 10.1006/viro.1993.1413. [DOI] [PubMed] [Google Scholar]
  2. Baba T. W., Koch J., Mittler E. S., Greene M., Wyand M., Penninck D., Ruprecht R. M. Mucosal infection of neonatal rhesus monkeys with cell-free SIV. AIDS Res Hum Retroviruses. 1994 Apr;10(4):351–357. doi: 10.1089/aid.1994.10.351. [DOI] [PubMed] [Google Scholar]
  3. Burns D. P., Desrosiers R. C. Selection of genetic variants of simian immunodeficiency virus in persistently infected rhesus monkeys. J Virol. 1991 Apr;65(4):1843–1854. doi: 10.1128/jvi.65.4.1843-1854.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davison-Fairburn B., Blanchard J., Hu F. S., Martin L., Harrison R., Ratterree M., Murphey-Corb M. Experimental infection of timed-pregnant rhesus monkeys with simian immunodeficiency virus (SIV) during early, middle, and late gestation. J Med Primatol. 1990;19(3-4):381–393. [PubMed] [Google Scholar]
  5. Fazely F., Sharma P. L., Fratazzi C., Greene M. F., Wyand M. S., Memon M. A., Penninck D., Ruprecht R. M. Simian immunodeficiency virus infection via amniotic fluid: a model to study fetal immunopathogenesis and prophylaxis. J Acquir Immune Defic Syndr. 1993 Feb;6(2):107–114. [PubMed] [Google Scholar]
  6. Gupta P., Urbach A., Cosentino L., Thampatty P., Brady M. HIV-1 isolates from children with or without AIDS have similar in vitro biologic properties. AIDS. 1993 Dec;7(12):1561–1564. doi: 10.1097/00002030-199312000-00003. [DOI] [PubMed] [Google Scholar]
  7. McGann K. A., Collman R., Kolson D. L., Gonzalez-Scarano F., Coukos G., Coutifaris C., Strauss J. F., Nathanson N. Human immunodeficiency virus type 1 causes productive infection of macrophages in primary placental cell cultures. J Infect Dis. 1994 Apr;169(4):746–753. doi: 10.1093/infdis/169.4.746. [DOI] [PubMed] [Google Scholar]
  8. Ochs H. D., Morton W. R., Tsai C. C., Thouless M. E., Zhu Q., Kuller L. D., Wu Y. P., Benveniste R. E. Maternal-fetal transmission of SIV in macaques: disseminated adenovirus infection in an offspring with congenital SIV infection. J Med Primatol. 1991 Jun;20(4):193–200. [PubMed] [Google Scholar]
  9. Overbaugh J., Rudensey L. M., Papenhausen M. D., Benveniste R. E., Morton W. R. Variation in simian immunodeficiency virus env is confined to V1 and V4 during progression to simian AIDS. J Virol. 1991 Dec;65(12):7025–7031. doi: 10.1128/jvi.65.12.7025-7031.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Scarlatti G., Hodara V., Rossi P., Muggiasca L., Bucceri A., Albert J., Fenyö E. M. Transmission of human immunodeficiency virus type 1 (HIV-1) from mother to child correlates with viral phenotype. Virology. 1993 Dec;197(2):624–629. doi: 10.1006/viro.1993.1637. [DOI] [PubMed] [Google Scholar]
  11. Scarlatti G., Leitner T., Halapi E., Wahlberg J., Marchisio P., Clerici-Schoeller M. A., Wigzell H., Fenyö E. M., Albert J., Uhlén M. Comparison of variable region 3 sequences of human immunodeficiency virus type 1 from infected children with the RNA and DNA sequences of the virus populations of their mothers. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1721–1725. doi: 10.1073/pnas.90.5.1721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scott G. B., Hutto C., Makuch R. W., Mastrucci M. T., O'Connor T., Mitchell C. D., Trapido E. J., Parks W. P. Survival in children with perinatally acquired human immunodeficiency virus type 1 infection. N Engl J Med. 1989 Dec 28;321(26):1791–1796. doi: 10.1056/NEJM198912283212604. [DOI] [PubMed] [Google Scholar]
  13. Sharma D. P., Zink M. C., Anderson M., Adams R., Clements J. E., Joag S. V., Narayan O. Derivation of neurotropic simian immunodeficiency virus from exclusively lymphocytetropic parental virus: pathogenesis of infection in macaques. J Virol. 1992 Jun;66(6):3550–3556. doi: 10.1128/jvi.66.6.3550-3556.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spouge J. L. Statistical analysis of sparse infection data and its implications for retroviral treatment trials in primates. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7581–7585. doi: 10.1073/pnas.89.16.7581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wolinsky S. M., Wike C. M., Korber B. T., Hutto C., Parks W. P., Rosenblum L. L., Kunstman K. J., Furtado M. R., Muñoz J. L. Selective transmission of human immunodeficiency virus type-1 variants from mothers to infants. Science. 1992 Feb 28;255(5048):1134–1137. doi: 10.1126/science.1546316. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES