Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1995 Dec;69(12):8046–8050. doi: 10.1128/jvi.69.12.8046-8050.1995

Preferential usage of the T-cell receptor by influenza virus hemagglutinin-specific human CD4+ T lymphocytes: in vitro life span of clonotypic T cells.

A Prévost-Blondel 1, D Chassin 1, D Zeliszewski 1, I Dorval 1, G Sterkers 1, C Pannetier 1, J G Guillet 1
PMCID: PMC189751  PMID: 7494319

Abstract

Human helper T-cell (Th) responses to influenza A virus were studied by analyzing T-cell receptor V beta gene diversity in hemagglutinin-specific Th lymphocytes. The T-lymphocyte population from peripheral blood became quickly oligoclonal when stimulated in vitro with the HA306-329 peptide, and T-cell receptor V beta 3 genes were mainly expanded. Moreover, specific junctional region oligonucleotide probes corresponding to hemagglutinin-specific clones were used to estimate temporal diversity during antigenic stimulations.

Full Text

The Full Text of this article is available as a PDF (218.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Argaet V. P., Schmidt C. W., Burrows S. R., Silins S. L., Kurilla M. G., Doolan D. L., Suhrbier A., Moss D. J., Kieff E., Sculley T. B. Dominant selection of an invariant T cell antigen receptor in response to persistent infection by Epstein-Barr virus. J Exp Med. 1994 Dec 1;180(6):2335–2340. doi: 10.1084/jem.180.6.2335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beverley P. C. Is T-cell memory maintained by crossreactive stimulation? Immunol Today. 1990 Jun;11(6):203–205. doi: 10.1016/0167-5699(90)90083-l. [DOI] [PubMed] [Google Scholar]
  3. Boitel B., Ermonval M., Panina-Bordignon P., Mariuzza R. A., Lanzavecchia A., Acuto O. Preferential V beta gene usage and lack of junctional sequence conservation among human T cell receptors specific for a tetanus toxin-derived peptide: evidence for a dominant role of a germline-encoded V region in antigen/major histocompatibility complex recognition. J Exp Med. 1992 Mar 1;175(3):765–777. doi: 10.1084/jem.175.3.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bowness P., Moss P. A., Rowland-Jones S., Bell J. I., McMichael A. J. Conservation of T cell receptor usage by HLA B27-restricted influenza-specific cytotoxic T lymphocytes suggests a general pattern for antigen-specific major histocompatibility complex class I-restricted responses. Eur J Immunol. 1993 Jul;23(7):1417–1421. doi: 10.1002/eji.1830230702. [DOI] [PubMed] [Google Scholar]
  5. Casanova J. L., Pannetier C., Jaulin C., Kourilsky P. Optimal conditions for directly sequencing double-stranded PCR products with sequenase. Nucleic Acids Res. 1990 Jul 11;18(13):4028–4028. doi: 10.1093/nar/18.13.4028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  7. Doherty P. C., Allan W., Eichelberger M., Carding S. R. Roles of alpha beta and gamma delta T cell subsets in viral immunity. Annu Rev Immunol. 1992;10:123–151. doi: 10.1146/annurev.iy.10.040192.001011. [DOI] [PubMed] [Google Scholar]
  8. Genevée C., Diu A., Nierat J., Caignard A., Dietrich P. Y., Ferradini L., Roman-Roman S., Triebel F., Hercend T. An experimentally validated panel of subfamily-specific oligonucleotide primers (V alpha 1-w29/V beta 1-w24) for the study of human T cell receptor variable V gene segment usage by polymerase chain reaction. Eur J Immunol. 1992 May;22(5):1261–1269. doi: 10.1002/eji.1830220522. [DOI] [PubMed] [Google Scholar]
  9. Jameson S. C., Bevan M. J. T cell receptor antagonists and partial agonists. Immunity. 1995 Jan;2(1):1–11. doi: 10.1016/1074-7613(95)90074-8. [DOI] [PubMed] [Google Scholar]
  10. Kalams S. A., Johnson R. P., Trocha A. K., Dynan M. J., Ngo H. S., D'Aquila R. T., Kurnick J. T., Walker B. D. Longitudinal analysis of T cell receptor (TCR) gene usage by human immunodeficiency virus 1 envelope-specific cytotoxic T lymphocyte clones reveals a limited TCR repertoire. J Exp Med. 1994 Apr 1;179(4):1261–1271. doi: 10.1084/jem.179.4.1261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lamb J. R., Eckels D. D., Lake P., Woody J. N., Green N. Human T-cell clones recognize chemically synthesized peptides of influenza haemagglutinin. Nature. 1982 Nov 4;300(5887):66–69. doi: 10.1038/300066a0. [DOI] [PubMed] [Google Scholar]
  12. Laver W. G., Gerhard W., Webster R. G., Frankel M. E., Air G. M. Antigenic drift in type A influenza virus: peptide mapping and antigenic analysis of A/PR/8/34 (HON1) variants selected with monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1425–1429. doi: 10.1073/pnas.76.3.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lehner P. J., Wang E. C., Moss P. A., Williams S., Platt K., Friedman S. M., Bell J. I., Borysiewicz L. K. Human HLA-A0201-restricted cytotoxic T lymphocyte recognition of influenza A is dominated by T cells bearing the V beta 17 gene segment. J Exp Med. 1995 Jan 1;181(1):79–91. doi: 10.1084/jem.181.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moss P. A., Moots R. J., Rosenberg W. M., Rowland-Jones S. J., Bodmer H. C., McMichael A. J., Bell J. I. Extensive conservation of alpha and beta chains of the human T-cell antigen receptor recognizing HLA-A2 and influenza A matrix peptide. Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):8987–8990. doi: 10.1073/pnas.88.20.8987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Okamoto Y., Gagnon S. J., Kurane I., Leporati A. M., Ennis F. A. Preferential usage of T-cell receptor V beta 17 by dengue virus-specific human T lymphocytes in a donor with immunity to dengue virus type 4. J Virol. 1994 Nov;68(11):7614–7619. doi: 10.1128/jvi.68.11.7614-7619.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ostrov D., Krieger J., Sidney J., Sette A., Concannon P. T cell receptor antagonism mediated by interaction between T cell receptor junctional residues and peptide antigen analogues. J Immunol. 1993 May 15;150(10):4277–4283. [PubMed] [Google Scholar]
  17. Pannetier C., Cochet M., Darche S., Casrouge A., Zöller M., Kourilsky P. The sizes of the CDR3 hypervariable regions of the murine T-cell receptor beta chains vary as a function of the recombined germ-line segments. Proc Natl Acad Sci U S A. 1993 May 1;90(9):4319–4323. doi: 10.1073/pnas.90.9.4319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Puisieux I., Even J., Pannetier C., Jotereau F., Favrot M., Kourilsky P. Oligoclonality of tumor-infiltrating lymphocytes from human melanomas. J Immunol. 1994 Sep 15;153(6):2807–2818. [PubMed] [Google Scholar]
  19. Snoke K., Alexander J., Franco A., Smith L., Brawley J. V., Concannon P., Grey H. M., Sette A., Wentworth P. The inhibition of different T cell lines specific for the same antigen with TCR antagonist peptides. J Immunol. 1993 Dec 15;151(12):6815–6821. [PubMed] [Google Scholar]
  20. Wedderburn L. R., Searle S. J., Rees A. R., Lamb J. R., Owen M. J. Mapping T cell recognition: the identification of a T cell receptor residue critical to the specific interaction with an influenza hemagglutinin peptide. Eur J Immunol. 1995 Jun;25(6):1654–1662. doi: 10.1002/eji.1830250627. [DOI] [PubMed] [Google Scholar]
  21. Zeliszewski D., Golvano J. J., Gaudebout P., Dorval I., Freidel C., Gebuhrer L., Betuel H., Borras-Cuesta F., Sterkers G. Implication of HLA-DR residues at positions 67, 71, and 86 in interaction between HLA-DR11 and peptide HA306-320. J Immunol. 1993 Dec 1;151(11):6237–6247. [PubMed] [Google Scholar]
  22. Zeliszewski D., Tiercy J. M., Dorval I., Kaplan C., Mach B., Sterkers G. DR-restricted T-cell reactivities associated with the Dw19 specificity can be directed against the products of either locus DRB3 (DRw52c) or locus DRB1. Hum Immunol. 1990 Aug;28(4):345–353. doi: 10.1016/0198-8859(90)90029-o. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES