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. 1995 Dec;69(12):8057–8060. doi: 10.1128/jvi.69.12.8057-8060.1995

Binding motifs predict major histocompatibility complex class II-restricted epitopes in the Sendai virus M protein.

G A Cole 1, T Tao 1, T L Hogg 1, K W Ryan 1, D L Woodland 1
PMCID: PMC189753  PMID: 7494321

Abstract

Major histocompatibility complex (MHC) class I ligand motifs have been defined for a number of class I molecules and have been successfully used to identify class I-restricted cytotoxic T-cell epitopes. In contrast, the relative degeneracy of sequence motifs in naturally processed MHC class II ligands has suggested that they may be of more limited use. Here, we use a predicted I-Ab ligand motif to identify antigenic peptides in the Sendai virus Enders strain matrix (M) protein. The entire coding sequence of the M protein was derived, and seven peptide sequences that contained the predicted I-Ab motif were identified. Analysis of I-Ab-restricted M-specific T-cell hybridomas for reactivity to these synthetic peptides identified two distinct epitopes. These data demonstrate that MHC class II motifs can be valuable in predicting T-cell epitopes.

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Selected References

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