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. 1996 Jan;70(1):590–594. doi: 10.1128/jvi.70.1.590-594.1996

Equine infectious anemia virus replication is upregulated during differentiation of blood monocytes from acutely infected horses.

D C Sellon 1, K M Walker 1, K E Russell 1, S T Perry 1, P Covington 1, F J Fuller 1
PMCID: PMC189850  PMID: 8523576

Abstract

Equine infectious anemia virus is a lentivirus that replicates in mature tissue macrophages of horses. Ponies were infected with equine infectious anemia virus. During febrile episodes, proviral DNA was detectable, but viral mRNA was not detectable. As cultured blood monocytes from these ponies differentiated into macrophages, viral expression was upregulated. In situ hybridization confirmed that viral transcription occurred in mature macrophages.

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Selected References

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  1. Clabough D. L., Gebhard D., Flaherty M. T., Whetter L. E., Perry S. T., Coggins L., Fuller F. J. Immune-mediated thrombocytopenia in horses infected with equine infectious anemia virus. J Virol. 1991 Nov;65(11):6242–6251. doi: 10.1128/jvi.65.11.6242-6251.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gendelman H. E., Narayan O., Kennedy-Stoskopf S., Kennedy P. G., Ghotbi Z., Clements J. E., Stanley J., Pezeshkpour G. Tropism of sheep lentiviruses for monocytes: susceptibility to infection and virus gene expression increase during maturation of monocytes to macrophages. J Virol. 1986 Apr;58(1):67–74. doi: 10.1128/jvi.58.1.67-74.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kono Y., Fukunaga Y., Kobayashi K. Excretion of equine infectious anemia virus from horses infected with the virus. Natl Inst Anim Health Q (Tokyo) 1973 Winter;13(4):182–186. [PubMed] [Google Scholar]
  4. Kono Y., Hirasawa K., Fukunaga Y., Taniguchi T. Recrudescence of equine infectious anemia by treatment with immunosuppressive drugs. Natl Inst Anim Health Q (Tokyo) 1976 Spring;16(1):8–15. [PubMed] [Google Scholar]
  5. Kono Y. Viremia and immunological responses in horses infected with equine infectious anemia virus. Natl Inst Anim Health Q (Tokyo) 1969 Spring;9(1):1–9. [PubMed] [Google Scholar]
  6. Levy J. A. Pathogenesis of human immunodeficiency virus infection. Microbiol Rev. 1993 Mar;57(1):183–289. doi: 10.1128/mr.57.1.183-289.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Maury W. Monocyte maturation controls expression of equine infectious anemia virus. J Virol. 1994 Oct;68(10):6270–6279. doi: 10.1128/jvi.68.10.6270-6279.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Montelaro R. C., Parekh B., Orrego A., Issel C. J. Antigenic variation during persistent infection by equine infectious anemia virus, a retrovirus. J Biol Chem. 1984 Aug 25;259(16):10539–10544. [PubMed] [Google Scholar]
  9. Narayan O., Kennedy-Stoskopf S., Sheffer D., Griffin D. E., Clements J. E. Activation of caprine arthritis-encephalitis virus expression during maturation of monocytes to macrophages. Infect Immun. 1983 Jul;41(1):67–73. doi: 10.1128/iai.41.1.67-73.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. O'Rourke K. I., Besola M. L., McGuire T. C. Proviral sequences detected by polymerase chain reaction in peripheral blood cells of horses with equine infectious anemia lentivirus. Arch Virol. 1991;117(1-2):109–119. doi: 10.1007/BF01310496. [DOI] [PubMed] [Google Scholar]
  11. Pauza C. D., Galindo J., Richman D. D. Human immunodeficiency virus infection of monoblastoid cells: cellular differentiation determines the pattern of virus replication. J Virol. 1988 Oct;62(10):3558–3564. doi: 10.1128/jvi.62.10.3558-3564.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Perry S. T., Flaherty M. T., Kelley M. J., Clabough D. L., Tronick S. R., Coggins L., Whetter L., Lengel C. R., Fuller F. The surface envelope protein gene region of equine infectious anemia virus is not an important determinant of tropism in vitro. J Virol. 1992 Jul;66(7):4085–4097. doi: 10.1128/jvi.66.7.4085-4097.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Perryman L. E., O'Rourke K. I., McGuire T. C. Immune responses are required to terminate viremia in equine infectious anemia lentivirus infection. J Virol. 1988 Aug;62(8):3073–3076. doi: 10.1128/jvi.62.8.3073-3076.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rice N. R., Lequarre A. S., Casey J. W., Lahn S., Stephens R. M., Edwards J. Viral DNA in horses infected with equine infectious anemia virus. J Virol. 1989 Dec;63(12):5194–5200. doi: 10.1128/jvi.63.12.5194-5200.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rwambo P. M., Issel C. J., Adams W. V., Jr, Hussain K. A., Miller M., Montelaro R. C. Equine infectious anemia virus (EIAV) humoral responses of recipient ponies and antigenic variation during persistent infection. Arch Virol. 1990;111(3-4):199–212. doi: 10.1007/BF01311054. [DOI] [PubMed] [Google Scholar]
  16. Salinovich O., Payne S. L., Montelaro R. C., Hussain K. A., Issel C. J., Schnorr K. L. Rapid emergence of novel antigenic and genetic variants of equine infectious anemia virus during persistent infection. J Virol. 1986 Jan;57(1):71–80. doi: 10.1128/jvi.57.1.71-80.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sellon D. C., Cullen J. M., Whetter L. E., Gebhard D. H., Coggins L., Fuller F. J. Production and characterization of a monoclonal antibody recognizing a cytoplasmic antigen of equine mononuclear phagocytes. Vet Immunol Immunopathol. 1993 May;36(4):303–318. doi: 10.1016/0165-2427(93)90027-2. [DOI] [PubMed] [Google Scholar]
  18. Sellon D. C., Fuller F. J., McGuire T. C. The immunopathogenesis of equine infectious anemia virus. Virus Res. 1994 May;32(2):111–138. doi: 10.1016/0168-1702(94)90038-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sellon D. C., Perry S. T., Coggins L., Fuller F. J. Wild-type equine infectious anemia virus replicates in vivo predominantly in tissue macrophages, not in peripheral blood monocytes. J Virol. 1992 Oct;66(10):5906–5913. doi: 10.1128/jvi.66.10.5906-5913.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shattock R. J., Friedland J. S., Griffin G. E. Release of human immunodeficiency virus by THP-1 cells and human macrophages is regulated by cellular adherence and activation. J Virol. 1993 Jun;67(6):3569–3575. doi: 10.1128/jvi.67.6.3569-3575.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stephens R. M., Casey J. W., Rice N. R. Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus. Science. 1986 Feb 7;231(4738):589–594. doi: 10.1126/science.3003905. [DOI] [PubMed] [Google Scholar]
  22. Valentin A., Von Gegerfelt A., Matsuda S., Nilsson K., Asjö B. In vitro maturation of mononuclear phagocytes and susceptibility to HIV-1 infection. J Acquir Immune Defic Syndr. 1991;4(8):751–759. [PubMed] [Google Scholar]
  23. Whetter L., Archambault D., Perry S., Gazit A., Coggins L., Yaniv A., Clabough D., Dahlberg J., Fuller F., Tronick S. Equine infectious anemia virus derived from a molecular clone persistently infects horses. J Virol. 1990 Dec;64(12):5750–5756. doi: 10.1128/jvi.64.12.5750-5756.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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