Abstract
The roles of the 3'- and 5'-terminal nucleotides and the panhandle structure of influenza B virus virion RNA were analyzed in vitro by transcription of model RNA templates with influenza B virus RNA polymerase. The results suggest that the stability of the panhandle and breathing of the extreme ends of the panhandle are important factors for efficient transcription. Influenza B virus polymerase appears to be more tolerant of mutations in the panhandle structure than influenza A virus polymerase. This is consistent with the greater degree of heterogeneity observed naturally in the 3'-terminal nucleotides of the virion RNA of influenza B virus than in influenza A virus.
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- Air G. M., Laver W. G., Luo M., Stray S. J., Legrone G., Webster R. G. Antigenic, sequence, and crystal variation in influenza B neuraminidase. Virology. 1990 Aug;177(2):578–587. doi: 10.1016/0042-6822(90)90523-t. [DOI] [PubMed] [Google Scholar]
- Barclay W. S., Palese P. Influenza B viruses with site-specific mutations introduced into the HA gene. J Virol. 1995 Feb;69(2):1275–1279. doi: 10.1128/jvi.69.2.1275-1279.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeBorde D. C., Donabedian A. M., Herlocher M. L., Naeve C. W., Maassab H. F. Sequence comparison of wild-type and cold-adapted B/Ann Arbor/1/66 influenza virus genes. Virology. 1988 Apr;163(2):429–443. doi: 10.1016/0042-6822(88)90284-x. [DOI] [PubMed] [Google Scholar]
- Desselberger U., Racaniello V. R., Zazra J. J., Palese P. The 3' and 5'-terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene. 1980 Feb;8(3):315–328. doi: 10.1016/0378-1119(80)90007-4. [DOI] [PubMed] [Google Scholar]
- Fodor E., Pritlove D. C., Brownlee G. G. Characterization of the RNA-fork model of virion RNA in the initiation of transcription in influenza A virus. J Virol. 1995 Jul;69(7):4012–4019. doi: 10.1128/jvi.69.7.4012-4019.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fodor E., Pritlove D. C., Brownlee G. G. The influenza virus panhandle is involved in the initiation of transcription. J Virol. 1994 Jun;68(6):4092–4096. doi: 10.1128/jvi.68.6.4092-4096.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fodor E., Seong B. L., Brownlee G. G. Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. J Gen Virol. 1993 Jul;74(Pt 7):1327–1333. doi: 10.1099/0022-1317-74-7-1327. [DOI] [PubMed] [Google Scholar]
- García-Sastre A., Palese P. Genetic manipulation of negative-strand RNA virus genomes. Annu Rev Microbiol. 1993;47:765–790. doi: 10.1146/annurev.mi.47.100193.004001. [DOI] [PubMed] [Google Scholar]
- Hagen M., Chung T. D., Butcher J. A., Krystal M. Recombinant influenza virus polymerase: requirement of both 5' and 3' viral ends for endonuclease activity. J Virol. 1994 Mar;68(3):1509–1515. doi: 10.1128/jvi.68.3.1509-1515.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall C. B., Douglas R. G., Jr, Geiman J. M., Meagher M. P. Viral shedding patterns of children with influenza B infection. J Infect Dis. 1979 Oct;140(4):610–613. doi: 10.1093/infdis/140.4.610. [DOI] [PubMed] [Google Scholar]
- Kemdirim S., Palefsky J., Briedis D. J. Influenza B virus PB1 protein; nucleotide sequence of the genome RNA segment predicts a high degree of structural homology with the corresponding influenza A virus polymerase protein. Virology. 1986 Jul 15;152(1):126–135. doi: 10.1016/0042-6822(86)90378-8. [DOI] [PubMed] [Google Scholar]
- Lamb R. A., Choppin P. W. The gene structure and replication of influenza virus. Annu Rev Biochem. 1983;52:467–506. doi: 10.1146/annurev.bi.52.070183.002343. [DOI] [PubMed] [Google Scholar]
- Lamb R. A., Horvath C. M. Diversity of coding strategies in influenza viruses. Trends Genet. 1991 Aug;7(8):261–266. doi: 10.1016/0168-9525(91)90326-L. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Li X., Palese P. Characterization of the polyadenylation signal of influenza virus RNA. J Virol. 1994 Feb;68(2):1245–1249. doi: 10.1128/jvi.68.2.1245-1249.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luo G. X., Luytjes W., Enami M., Palese P. The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure. J Virol. 1991 Jun;65(6):2861–2867. doi: 10.1128/jvi.65.6.2861-2867.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCauley J. W., Mahy B. W. Structure and function of the influenza virus genome. Biochem J. 1983 May 1;211(2):281–294. doi: 10.1042/bj2110281. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nolan T. F., Jr, Goodman R. A., Hinman A. R., Noble G. R., Kendal A. P., Thacker S. B. Morbidity and mortality associated with influenza B in the United States, 1979-1980. A report from the Center for Disease Control. J Infect Dis. 1980 Sep;142(3):360–362. doi: 10.1093/infdis/142.3.360. [DOI] [PubMed] [Google Scholar]
- Piccone M. E., Fernandez-Sesma A., Palese P. Mutational analysis of the influenza virus vRNA promoter. Virus Res. 1993 May;28(2):99–112. doi: 10.1016/0168-1702(93)90129-b. [DOI] [PubMed] [Google Scholar]
- Seong B. L., Brownlee G. G. A new method for reconstituting influenza polymerase and RNA in vitro: a study of the promoter elements for cRNA and vRNA synthesis in vitro and viral rescue in vivo. Virology. 1992 Jan;186(1):247–260. doi: 10.1016/0042-6822(92)90079-5. [DOI] [PubMed] [Google Scholar]
- Seong B. L., Brownlee G. G. Nucleotides 9 to 11 of the influenza A virion RNA promoter are crucial for activity in vitro. J Gen Virol. 1992 Dec;73(Pt 12):3115–3124. doi: 10.1099/0022-1317-73-12-3115. [DOI] [PubMed] [Google Scholar]
- Seong B. L. Influencing the influenza virus: genetic analysis and engineering of the negative-sense RNA genome. Infect Agents Dis. 1993 Feb;2(1):17–24. [PubMed] [Google Scholar]
- Shaw M. W., Arden N. H., Maassab H. F. New aspects of influenza viruses. Clin Microbiol Rev. 1992 Jan;5(1):74–92. doi: 10.1128/cmr.5.1.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
- Stoeckle M. Y., Shaw M. W., Choppin P. W. Segment-specific and common nucleotide sequences in the noncoding regions of influenza B virus genome RNAs. Proc Natl Acad Sci U S A. 1987 May;84(9):2703–2707. doi: 10.1073/pnas.84.9.2703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tiley L. S., Hagen M., Matthews J. T., Krystal M. Sequence-specific binding of the influenza virus RNA polymerase to sequences located at the 5' ends of the viral RNAs. J Virol. 1994 Aug;68(8):5108–5116. doi: 10.1128/jvi.68.8.5108-5116.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Varich N. L., Gubareva L. V., Kaverin N. V. Influenza B virus mRNA synthesis in vivo: efficient transcription of vRNAs 1, 2 and 3. J Gen Virol. 1990 Sep;71(Pt 9):2171–2174. doi: 10.1099/0022-1317-71-9-2171. [DOI] [PubMed] [Google Scholar]
- Yamashita M., Krystal M., Fitch W. M., Palese P. Influenza B virus evolution: co-circulating lineages and comparison of evolutionary pattern with those of influenza A and C viruses. Virology. 1988 Mar;163(1):112–122. doi: 10.1016/0042-6822(88)90238-3. [DOI] [PubMed] [Google Scholar]