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. 1987 Apr;127(1):9–14.

Extracellular matrix does not induce the proliferation, but promotes the differentiation, of Hodgkin's cell line HDLM-1.

S M Hsu, X Zhao, P L Hsu, M S Lok
PMCID: PMC1899592  PMID: 3565539

Abstract

Tumor cells from the Hodgkin's cell line HDLM-1 were cultured in extracellular matrix (ECM)-coated flasks. Within 24 hours, the cells underwent rapid differentiation, accompanied by morphologic and phenotypic changes. A slow and less prominent, but similar change was observed in HDLM-1 cells after they had been induced with phorbol ester for 5 days. The ECM-induced cells became HeFi-1-negative and expressed antigens 2H9 and 1E9 on their nuclear membranes. These cells had punctate acid-phosphatase and esterase activities. Approximately 70% of the ECM-induced cells were elongated and had long, blunt cytoplasmic projections. These findings, together with evidence the authors previously presented, indicate that H-RS cells are related to interdigitating reticulum cells. The authors believe that the ECM-induced HDLM-1 cells can be used as an important model for studies of the nature and cell lineage of Hodgkin's disease.

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Selected References

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  1. Beckstead J. H., Warnke R., Bainton D. F. Histochemistry of Hodgkin's disease. Cancer Treat Rep. 1982 Apr;66(4):609–613. [PubMed] [Google Scholar]
  2. Crickard K., Crickard U., Yoonessi M. Human ovarian carcinoma cells maintained on extracellular matrix versus plastic. Cancer Res. 1983 Jun;43(6):2762–2767. [PubMed] [Google Scholar]
  3. Diehl V., Kirchner H. H., Burrichter H., Stein H., Fonatsch C., Gerdes J., Schaadt M., Heit W., Uchanska-Ziegler B., Ziegler A. Characteristics of Hodgkin's disease-derived cell lines. Cancer Treat Rep. 1982 Apr;66(4):615–632. [PubMed] [Google Scholar]
  4. Drexler H. G., Gaedicke G., Lok M. S., Diehl V., Minowada J. Hodgkin's disease derived cell lines HDLM-2 and L-428: comparison of morphology, immunological and isoenzyme profiles. Leuk Res. 1986;10(5):487–500. doi: 10.1016/0145-2126(86)90084-6. [DOI] [PubMed] [Google Scholar]
  5. Gospodarowicz D., Lui G. M., Gonzalez R. High-density lipoproteins and the proliferation of human tumor cells maintained on extracellular matrix-coated dishes and exposed to defined medium. Cancer Res. 1982 Sep;42(9):3704–3713. [PubMed] [Google Scholar]
  6. Hecht T. T., Longo D. L., Cossman J., Bolen J. B., Hsu S. M., Israel M., Fisher R. I. Production and characterization of a monoclonal antibody that binds Reed-Sternberg cells. J Immunol. 1985 Jun;134(6):4231–4236. [PubMed] [Google Scholar]
  7. Hsu S. M., Ho Y. S., Li P. J., Monheit J., Ree H. J., Sheibani K., Winberg C. D. L&H variants of Reed-Sternberg cells express sialylated Leu M1 antigen. Am J Pathol. 1986 Feb;122(2):199–203. [PMC free article] [PubMed] [Google Scholar]
  8. Hsu S. M., Hsu P. L. Phenotypes and phorbol ester-induced differentiation of human histiocytic lymphoma cell lines (U-937 and SU-DHL-1) and Reed-Sternberg cells. Am J Pathol. 1986 Feb;122(2):223–230. [PMC free article] [PubMed] [Google Scholar]
  9. Hsu S. M., Huang L. C., Hsu P. L., Ge Z. H., Ho Y. S., Cuttita F., Mulshine J. Biochemical and ultrastructural study of Leu M1 antigen in Reed-Sternberg cells: comparison with granulocytes and interdigitating reticulum cells. J Natl Cancer Inst. 1986 Aug;77(2):363–370. [PubMed] [Google Scholar]
  10. Hsu S. M., Jaffe E. S. Leu M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's disease. Am J Clin Pathol. 1984 Jul;82(1):29–32. doi: 10.1093/ajcp/82.1.29. [DOI] [PubMed] [Google Scholar]
  11. Hsu S. M., Jaffe E. S. Phenotypic expression of T lymphocytes in thymus and peripheral lymphoid tissues. Am J Pathol. 1985 Oct;121(1):69–78. [PMC free article] [PubMed] [Google Scholar]
  12. Hsu S. M., Pescovitz M. D., Hsu P. L. Monoclonal antibodies against SU-DHL-1 cells stain the neoplastic cells in true histiocytic lymphoma, malignant histiocytosis, and Hodgkin's disease. Blood. 1986 Jul;68(1):213–219. [PubMed] [Google Scholar]
  13. Hsu S. M. Phenotypic expression of B lymphocytes. III. Marginal zone B cells in the spleen are characterized by the expression of Tac and alkaline phosphatase. J Immunol. 1985 Jul;135(1):123–130. [PubMed] [Google Scholar]
  14. Hsu S. M., Raine L., Fanger H. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol. 1981 May;75(5):734–738. doi: 10.1093/ajcp/75.5.734. [DOI] [PubMed] [Google Scholar]
  15. Hsu S. M., Soban E. Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. J Histochem Cytochem. 1982 Oct;30(10):1079–1082. doi: 10.1177/30.10.6182185. [DOI] [PubMed] [Google Scholar]
  16. Hsu S. M., Yang K., Jaffe E. S. Hairy cell leukemia: a B cell neoplasm with a unique antigenic phenotype. Am J Clin Pathol. 1983 Oct;80(4):421–428. doi: 10.1093/ajcp/80.4.421. [DOI] [PubMed] [Google Scholar]
  17. Hsu S. M., Yang K., Jaffe E. S. Phenotypic expression of Hodgkin's and Reed-Sternberg cells in Hodgkin's disease. Am J Pathol. 1985 Feb;118(2):209–217. [PMC free article] [PubMed] [Google Scholar]
  18. Hsu S. M., Zhao X. Expression of interleukin-1 in Reed-Sternberg cells and neoplastic cells from true histiocytic malignancies. Am J Pathol. 1986 Nov;125(2):221–225. [PMC free article] [PubMed] [Google Scholar]
  19. Kadin M. E. Possible origin of the Reed-Sternberg cell from an interdigitating reticulum cell. Cancer Treat Rep. 1982 Apr;66(4):601–608. [PubMed] [Google Scholar]
  20. Lander A. D., Fujii D. K., Gospodarowicz D., Reichardt L. F. Characterization of a factor that promotes neurite outgrowth: evidence linking activity to a heparan sulfate proteoglycan. J Cell Biol. 1982 Sep;94(3):574–585. doi: 10.1083/jcb.94.3.574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sun T. T., Green H. Cultured epithelial cells of cornea, conjunctiva and skin: absence of marked intrinsic divergence of their differentiated states. Nature. 1977 Oct 6;269(5628):489–493. doi: 10.1038/269489a0. [DOI] [PubMed] [Google Scholar]
  22. Uchiyama T., Broder S., Waldmann T. A. A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. I. Production of anti-Tac monoclonal antibody and distribution of Tac (+) cells. J Immunol. 1981 Apr;126(4):1393–1397. [PubMed] [Google Scholar]
  23. Vlodavsky I., Lui G. M., Gospodarowicz D. Morphological appearance, growth behavior and migratory activity of human tumor cells maintained on extracellular matrix versus plastic. Cell. 1980 Mar;19(3):607–616. doi: 10.1016/s0092-8674(80)80037-7. [DOI] [PubMed] [Google Scholar]
  24. Yang J., Richards J., Bowman P., Guzman R., Enami J., McCormick K., Hamamoto S., Pitelka D., Nandi S. Sustained growth and three-dimensional organization of primary mammary tumor epithelial cells embedded in collagen gels. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3401–3405. doi: 10.1073/pnas.76.7.3401. [DOI] [PMC free article] [PubMed] [Google Scholar]

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