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. 1987 Apr;127(1):15–26.

Electrical charge and joint inflammation. Suppression of cationic aBSA-induced arthritis with a competitive polycation.

W B van den Berg, L A Joosten, L B van de Putte, W A Zwarts
PMCID: PMC1899603  PMID: 3565536

Abstract

Chronicity of murine allergic arthritis depends on the charge-mediated retention of the cationic antigen in the joint. The authors examined whether arthritis induced with the positively charged antigen amidated bovine serum albumin (aBSA) could be modulated with a nonimmunogenic polycation by competition for anionic retention sites in the joint. Concomitant intraarticular injection of aBSA with the cationic protein protamine chloride (pI approximately 10) strongly reduced the retention of aBSA. Detailed analysis revealed that the retention of aBSA in the noncartilaginous tissues was significantly reduced by protamine, whereas the retention in the highly negatively charged cartilage was completely prevented. Joint inflammation was already significantly suppressed at Day 3 and suppression was still demonstrable at Day 28. Protamine treatment also caused a highly significant reduction in cartilage damage and bone apposition. Control experiments indicated that the suppressive effect of protamine was related to its interference with antigen retention in the joint and not to a mere antiinflammatory action.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler S. G., Wang H., Ward H. J., Cohen A. H., Border W. A. Electrical charge. Its role in the pathogenesis and prevention of experimental membranous nephropathy in the rabbit. J Clin Invest. 1983 Mar;71(3):487–499. doi: 10.1172/JCI110793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Batsford S. R., Takamiya M., Vogt A. A model of in situ immune complex glomerulonephritis in the rat employing cationized ferritin. Clin Nephrol. 1980 Nov;14(5):211–216. [PubMed] [Google Scholar]
  3. Border W. A., Ward H. J., Kamil E. S., Cohen A. H. Induction of membranous nephropathy in rabbits by administration of an exogenous cationic antigen. J Clin Invest. 1982 Feb;69(2):451–461. doi: 10.1172/JCI110469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brackertz D., Mitchell G. F., Mackay I. R. Antigen-induced arthritis in mice. I. Induction of arthritis in various strains of mice. Arthritis Rheum. 1977 Apr;20(3):841–850. doi: 10.1002/art.1780200314. [DOI] [PubMed] [Google Scholar]
  5. Brackertz D., Mitchell G. F., Vadas M. A., Mackay I. R., Miller J. F. Studies on antigen-induced arthritis in mice. II. Immunologic correlates of arthritis susceptibility in mice. J Immunol. 1977 May;118(5):1639–1644. [PubMed] [Google Scholar]
  6. Cooke T. D., Hurd E. R., Ziff M., Jasin H. E. The pathogenesis of chronic inflammation in experimental antigen-induced arthritis. II. Preferential localization of antigen-antibody complexes to collagenous tissues. J Exp Med. 1972 Feb 1;135(2):323–338. doi: 10.1084/jem.135.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cooke T. D., Sumia M., Elliott S., Maeda M. Immune complex mediated destruction of cartilage in antigen-induced arthritis of rabbits. J Rheumatol Suppl. 1983 Dec;11:103–107. [PubMed] [Google Scholar]
  8. Danon D., Goldstein L., Marikovsky Y., Skutelsky E. Use of cationized ferritin as a label of negative charges on cell surfaces. J Ultrastruct Res. 1972 Mar;38(5):500–510. doi: 10.1016/0022-5320(72)90087-1. [DOI] [PubMed] [Google Scholar]
  9. Glynn L. E. The chronicity of inflammation and its significance in rheumatoid arthritis. Ann Rheum Dis. 1968 Mar;27(2):105–121. doi: 10.1136/ard.27.2.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  11. Hollister J. R., Mannik M. Antigen retention in joint tissues in antigen-induced synovitis. Clin Exp Immunol. 1974 Apr;16(4):615–627. [PMC free article] [PubMed] [Google Scholar]
  12. Keystone E. C., Schorlemmer H. U., Pope C., Allison A. C. Zymosan-induced arthritis: a model of chronic proliferative arthritis following activation of the alternative pathway of complement. Arthritis Rheum. 1977 Sep-Oct;20(7):1396–1401. doi: 10.1002/art.1780200714. [DOI] [PubMed] [Google Scholar]
  13. Kruijsen M. W., van den Berg W. B., van de Putte L. B. Influence of the severity and duration of murine antigen-induced arthritis on cartilage proteoglycan synthesis and chondrocyte death. Arthritis Rheum. 1985 Jul;28(7):813–819. doi: 10.1002/art.1780280713. [DOI] [PubMed] [Google Scholar]
  14. Kruijsen M. W., van den Berg W. B., van de Putte L. B. Sequential alterations of periarticular structures in antigen-induced arthritis in mice. Histological observations on fibrous capsule, ligaments, bone and muscles, using whole joint sections. Br J Exp Pathol. 1983 Jun;64(3):298–305. [PMC free article] [PubMed] [Google Scholar]
  15. Lens J. W., van den Berg W. B., van de Putte L. B. Flare-up of antigen-induced arthritis in mice after challenge with intravenous antigen: studies on the characteristics of and mechanisms involved in the reaction. Clin Exp Immunol. 1984 Feb;55(2):287–294. [PMC free article] [PubMed] [Google Scholar]
  16. Lens J. W., van den Berg W. B., van de Putte L. B. Quantitation of arthritis by 99mTc-uptake measurements in the mouse knee-joint: correlation with histological joint inflammation scores. Agents Actions. 1984 Jun;14(5-6):723–728. doi: 10.1007/BF01978915. [DOI] [PubMed] [Google Scholar]
  17. Lens J. W., van den Berg W. B., van de Putte L. B., Zwarts W. A. Flare of antigen-induced arthritis in mice after intravenous challenge. Kinetics of antigen in the circulation and localization of antigen in the arthritic and noninflamed joint. Arthritis Rheum. 1986 May;29(5):665–674. doi: 10.1002/art.1780290512. [DOI] [PubMed] [Google Scholar]
  18. Lens J. W., van den Berg W. B., van de Putte L. B., van den Bersselaar L. Flare-up of antigen-induced arthritis in mice after challenge with oral antigen. Clin Exp Immunol. 1984 Nov;58(2):364–371. [PMC free article] [PubMed] [Google Scholar]
  19. Maeda M., Cooke T. D. Destruction of rabbit knee hyaline cartilage associated with surface antigen-antibody interaction during the Arthus reaction of antigen-induced arthritis. Clin Orthop Relat Res. 1984 Nov;(190):287–291. [PubMed] [Google Scholar]
  20. Schalkwijk J., van den Berg W. B., van de Putte L. B., Joosten L. A., van den Bersselaar L. Cationization of catalase, peroxidase, and superoxide dismutase. Effect of improved intraarticular retention on experimental arthritis in mice. J Clin Invest. 1985 Jul;76(1):198–205. doi: 10.1172/JCI111946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schalkwijk J., van den Berg W. B., van de Putte L. B., Joosten L. A., van der Sluis M. Effects of experimental joint inflammation on bone marrow and periarticular bone. A study of two types of arthritis, using variable degrees of inflammation. Br J Exp Pathol. 1985 Aug;66(4):435–444. [PMC free article] [PubMed] [Google Scholar]
  22. van den Berg W. B., Kruijsen M. W., van de Putte L. B., van Beusekom H. J., van der Sluis-van der Pol M., Zwarts W. A. Antigen-induced and zymosan-induced arthritis in mice: studies on in vivo cartilage proteoglycan synthesis and chondrocyte death. Br J Exp Pathol. 1981 Jun;62(3):308–316. [PMC free article] [PubMed] [Google Scholar]
  23. van den Berg W. B., van Beusekom H. J., van de Putte L. B., Zwarts W. A., van der Sluis M. Antigen handling in antigen-induced arthritis in mice: an autoradiographic and immunofluorescence study using whole joint sections. Am J Pathol. 1982 Jul;108(1):9–16. [PMC free article] [PubMed] [Google Scholar]
  24. van den Berg W. B., van Lent P. L., van de Putte L. B., Zwarts W. A. Electrical charge of hyaline articular cartilage: its role in the retention of anionic and cationic proteins. Clin Immunol Immunopathol. 1986 May;39(2):187–197. doi: 10.1016/0090-1229(86)90083-8. [DOI] [PubMed] [Google Scholar]
  25. van den Berg W. B., van de Putte L. B. Electrical charge of the antigen determines its localization in the mouse knee joint. Deep penetration of cationic BSA in hyaline articular cartilage. Am J Pathol. 1985 Nov;121(2):224–234. [PMC free article] [PubMed] [Google Scholar]
  26. van den Berg W. B., van de Putte L. B., Zwarts W. A., Joosten L. A. Electrical charge of the antigen determines intraarticular antigen handling and chronicity of arthritis in mice. J Clin Invest. 1984 Nov;74(5):1850–1859. doi: 10.1172/JCI111604. [DOI] [PMC free article] [PubMed] [Google Scholar]

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