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. 1987 Apr;127(1):66–73.

The immunocytochemical distribution of leukocytic subpopulations in human endometrium.

B R Kamat, P G Isaacson
PMCID: PMC1899604  PMID: 3565538

Abstract

Thirty human endometria were selected from women aged 21-54 years who had undergone routine dilation and curettage procedures for tubal ligation, infertility dating, and irregular menstrual cycling. Histologic sections of the cases chosen were examined to exclude any major pathologic condition (including chronic endometritis). The specimens were stained with monoclonal antibodies to a common leukocytic antigen (H Leu-1 and PD7/26), pan-T-cell antigen (UCHT1), T helper/inducer and T suppressor/cytotoxic antigens (Leu-3a and UCHT4, respectively), pan-B cell antigen (To15 and Leu-12), and macrophage antigens (UCHM1 and Leu-M3). Other antibodies used included TAL-1B5 (anti-HLA-DR), Leu-7 (natural killer cell) and Na 1/34 (anti-T6/Langerhans/interdigitating reticulum cell). The endometria contained significant numbers of common leukocyte antigen-positive cells (occupying approximately 10-15% of the stroma), the numbers of which appeared to increase in the late secretory/pre-menstrual phase (20-25% of the stroma). The major leukocyte populations were T cells and macrophages; the latter, with neutrophils, appeared to account for the premenstrual increase in leukocytes. T cells were distributed both diffusely in the stroma and in periglandular stromal aggregates closely applied to the glands. The T8+ suppressor/cytotoxic population was predominant within the stromal nodules. In addition, scattered intraepithelial T suppressor/cytotoxic cells were present. Macrophages (UCHM1 and HLA-DR+) were also distributed diffusely in the stroma and as part of the periglandular stromal aggregates, in areas sending long cell processes into the epithelium. B cells appeared to be limited to scattered cells in the stroma, only increasing in number within lymphoid follicles. Natural killer cells, as defined by Leu-7+ cells, were also present, scattered singly in the stroma and within lymphoid follicles. The demonstration of large mononuclear dendritic-appearing Na 1/34+ cells within the glands of the endometrium in 5/30 cases suggests the presence of T6+ Langerhans/interdigitating reticulum cells in the endometrium. Thus, the normal endometrium has an important population of immunologically competent cells. Further study of these cell populations may elucidate their contribution, if any, to pathologic conditions in the endometrium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams T. E., Bodmer J. G., Bodmer W. F. Production and characterization of monoclonal antibodies recognizing the alpha-chain subunits of human ia alloantigens. Immunology. 1983 Dec;50(4):613–624. [PMC free article] [PubMed] [Google Scholar]
  2. Beverley P. C., Callard R. E. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981 Apr;11(4):329–334. doi: 10.1002/eji.1830110412. [DOI] [PubMed] [Google Scholar]
  3. Bienenstock J., Befus A. D. Mucosal immunology. Immunology. 1980 Oct;41(2):249–270. [PMC free article] [PubMed] [Google Scholar]
  4. Billington W. D. Immunological aspects of normal and abnormal pregnancy. Eur J Obstet Gynecol Reprod Biol. 1975;5(1-2):147–154. doi: 10.1016/0028-2243(75)90139-2. [DOI] [PubMed] [Google Scholar]
  5. Curran R. C., Gregory J. Effects of fixation and processing on immunohistochemical demonstration of immunoglobulin in paraffin sections of tonsil and bone marrow. J Clin Pathol. 1980 Nov;33(11):1047–1057. doi: 10.1136/jcp.33.11.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dimitriu-Bona A., Burmester G. R., Waters S. J., Winchester R. J. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol. 1983 Jan;130(1):145–152. [PubMed] [Google Scholar]
  7. Epenetos A. A., Bobrow L. G., Adams T. E., Collins C. M., Isaacson P. G., Bodmer W. F. A monoclonal antibody that detects HLA-D region antigen in routinely fixed, wax embedded sections of normal and neoplastic lymphoid tissues. J Clin Pathol. 1985 Jan;38(1):12–17. doi: 10.1136/jcp.38.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fithian E., Kung P., Goldstein G., Rubenfeld M., Fenoglio C., Edelson R. Reactivity of Langerhans cells with hybridoma antibody. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2541–2544. doi: 10.1073/pnas.78.4.2541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gatter K. C., Morris H. B., Roach B., Mortimer P., Fleming K. A., Mason D. Y. Langerhans' cells and T cells in human skin tumours: an immunohistological study. Histopathology. 1984 Mar;8(2):229–244. doi: 10.1111/j.1365-2559.1984.tb02338.x. [DOI] [PubMed] [Google Scholar]
  10. Kabawat S. E., Mostoufi-Zadeh M., Berkowitz R. S., Driscoll S. G., Goldstein D. P., Bhan A. K. Implantation site in complete molar pregnancy: a study of immunologically competent cells with monoclonal antibodies. Am J Obstet Gynecol. 1985 May 1;152(1):97–99. doi: 10.1016/s0002-9378(85)80188-5. [DOI] [PubMed] [Google Scholar]
  11. McMichael A. J., Pilch J. R., Galfré G., Mason D. Y., Fabre J. W., Milstein C. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur J Immunol. 1979 Mar;9(3):205–210. doi: 10.1002/eji.1830090307. [DOI] [PubMed] [Google Scholar]
  12. Morris H. H., Gatter K. C., Stein H., Mason D. Y. Langerhans' cells in human cervical epithelium: an immunohistological study. Br J Obstet Gynaecol. 1983 May;90(5):400–411. doi: 10.1111/j.1471-0528.1983.tb08935.x. [DOI] [PubMed] [Google Scholar]
  13. Morris H., Edwards J., Tiltman A., Emms M. Endometrial lymphoid tissue: an immunohistological study. J Clin Pathol. 1985 Jun;38(6):644–652. doi: 10.1136/jcp.38.6.644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RAHN J., UEBEL J. RUNDZELLENINFILTRATE DES ENDOMETRIUM UND IHRE NOSOLOGISCHE BEWERTUNG. Zentralbl Gynakol. 1965 May 29;87:737–745. [PubMed] [Google Scholar]
  15. Rahn J. Zur nosologischen Bewertung der Infiltrate des menschlichen Endometriums. Gegenbaurs Morphol Jahrb. 1968;111(4):605–609. [PubMed] [Google Scholar]
  16. Selby W. S., Janossy G., Jewell D. P. Immunohistological characterisation of intraepithelial lymphocytes of the human gastrointestinal tract. Gut. 1981 Mar;22(3):169–176. doi: 10.1136/gut.22.3.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Spencer J., Finn T., Isaacson P. G. Gut associated lymphoid tissue: a morphological and immunocytochemical study of the human appendix. Gut. 1985 Jul;26(7):672–679. doi: 10.1136/gut.26.7.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stein H., Gerdes J., Mason D. Y. The normal and malignant germinal centre. Clin Haematol. 1982 Oct;11(3):531–559. [PubMed] [Google Scholar]
  19. Wahab Z. A., Wright G. L., Jr Monoclonal antibody (anti-Leu 7) directed against natural killer cells reacts with normal, benign and malignant prostate tissues. Int J Cancer. 1985 Dec 15;36(6):677–683. doi: 10.1002/ijc.2910360610. [DOI] [PubMed] [Google Scholar]
  20. Warnke R. A., Gatter K. C., Falini B., Hildreth P., Woolston R. E., Pulford K., Cordell J. L., Cohen B., De Wolf-Peeters C., Mason D. Y. Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med. 1983 Nov 24;309(21):1275–1281. doi: 10.1056/NEJM198311243092102. [DOI] [PubMed] [Google Scholar]

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