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. 1996 Mar;70(3):1433–1438. doi: 10.1128/jvi.70.3.1433-1438.1996

Characterization of two novel YY1 binding sites in the polyomavirus late promoter.

F Martelli 1, C Iacobini 1, M Caruso 1, A Felsani 1
PMCID: PMC189963  PMID: 8627660

Abstract

NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, delta, NF-E1, UCRBP, or CF1. Moreover, we show the presence in the polyomavirus genome of a second DNA motif, located 40 bp from the first, which binds YY1/NF-D with high affinity. Both sites lie downstream of the major late transcription initiation sites. By site-directed mutagenesis, we demonstrate that both elements contribute positively to the activity of the late promoter, probably by a cooperative mechanism. We also demonstrate that the requirement of the YY1/NF-D function for late promoter activity varies with the cell line.

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Selected References

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