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. 1996 Mar;70(3):1455–1466. doi: 10.1128/jvi.70.3.1455-1466.1996

Investigation of the pathogenesis of transplacental transmission of Aleutian mink disease parvovirus in experimentally infected mink.

S Broll 1, S Alexandersen 1
PMCID: PMC189966  PMID: 8627663

Abstract

The transplacental transmission of Aleutian mink disease parvovirus (ADV) was studied in experimental infection of 1-year-old female non-Aleutian mink. The ADV-seronegative female mink were inoculated with ADV prior to mating or after the expected implantation of the embryos during pregnancy. A group of uninfected females served as a control group. Animals from each group were killed prior to or shortly after parturition. The in situ hybridization technique with radiolabeled strand-specific RNA probes was used to determine target cells of virus infection and virus replication. In both infected groups, ADV crossed the endotheliochorial placental barrier, although animals infected before mating already had high antibody titers against ADV at the time of implantation. The percentage of dead and resorbed fetuses was much higher in dams infected before mating. In the placentae of these mink, virus DNA and viral mRNA were detected in cells in the mesenchymal stroma of the placental labyrinth and hematoma but only occasionally in the cytotrophoblast of the placental hematoma. Placentae of animals infected during pregnancy showed in addition very high levels of virus and also viral replication in a large number of cytotrophoblast cells in the placental hematoma, which exhibited distinct inclusion bodies. In both groups, neither virus nor virus replication could be detected in maternal endothelial cells or fetal syncytiotrophoblast of the placental labyrinth. Fetuses were positive for virus and viral replication at high levels in a wide range of tissues. Possible routes of transplacental transmission of ADV and the role of trophoblast cells as targets for viral replication are discussed.

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Selected References

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  1. Alexandersen S. Acute interstitial pneumonia in mink kits: experimental reproduction of the disease. Vet Pathol. 1986 Sep;23(5):579–588. doi: 10.1177/030098588602300506. [DOI] [PubMed] [Google Scholar]
  2. Alexandersen S., Bloom M. E., Wolfinbarger J. Evidence of restricted viral replication in adult mink infected with Aleutian disease of mink parvovirus. J Virol. 1988 May;62(5):1495–1507. doi: 10.1128/jvi.62.5.1495-1507.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alexandersen S., Bloom M. E., Wolfinbarger J., Race R. E. In situ molecular hybridization for detection of Aleutian mink disease parvovirus DNA by using strand-specific probes: identification of target cells for viral replication in cell cultures and in mink kits with virus-induced interstitial pneumonia. J Virol. 1987 Aug;61(8):2407–2419. doi: 10.1128/jvi.61.8.2407-2419.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Alexandersen S., Storgaard T., Kamstrup N., Aasted B., Porter D. D. Pathogenesis of Aleutian mink disease parvovirus infection: effects of suppression of antibody response on viral mRNA levels and on development of acute disease. J Virol. 1994 Feb;68(2):738–749. doi: 10.1128/jvi.68.2.738-749.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Anderson J. W. Ultrastructure of the placenta and fetal membranes of the dog. I. The placental labyrinth. Anat Rec. 1969 Sep;165(1):15–35. doi: 10.1002/ar.1091650103. [DOI] [PubMed] [Google Scholar]
  6. Bloom M. E., Kanno H., Mori S., Wolfinbarger J. B. Aleutian mink disease: puzzles and paradigms. Infect Agents Dis. 1994 Dec;3(6):279–301. [PubMed] [Google Scholar]
  7. Bloom M. E., Race R. E., Wolfinbarger J. B. Analysis of Aleutian disease of mink parvovirus infection using strand-specific hybridization probes. Intervirology. 1987;27(2):102–111. doi: 10.1159/000149727. [DOI] [PubMed] [Google Scholar]
  8. Broll S., Waldvogel A. S., Rosskopf M., Corboz L., Pospischil A. Untersuchungen zu infektiösen Ursachen für Aborte und Totgeburten beim Schwein in der Schweiz. Zentralbl Veterinarmed B. 1993 Dec;40(9-10):641–653. [PubMed] [Google Scholar]
  9. Bychkova N. A. Morfologicheskaia i gistokhimicheskaia kharakteristika platsenty amerikanskoi norki. Arkh Anat Gistol Embriol. 1971 Aug;61(8):95–98. [PubMed] [Google Scholar]
  10. Clewley J. P., Cohen B. J., Field A. M. Detection of parvovirus B19 DNA, antigen, and particles in the human fetus. J Med Virol. 1987 Dec;23(4):367–376. doi: 10.1002/jmv.1890230409. [DOI] [PubMed] [Google Scholar]
  11. Cotmore S. F., Tattersall P. The autonomously replicating parvoviruses of vertebrates. Adv Virus Res. 1987;33:91–174. doi: 10.1016/s0065-3527(08)60317-6. [DOI] [PubMed] [Google Scholar]
  12. ENDERS A. C. Histological observations on the chorio-allantoic placenta of the mink. Anat Rec. 1957 Feb;127(2):231–245. doi: 10.1002/ar.1091270209. [DOI] [PubMed] [Google Scholar]
  13. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  14. Goldstein J., Braverman M., Salafia C., Buckley P. The phenotype of human placental macrophages and its variation with gestational age. Am J Pathol. 1988 Dec;133(3):648–659. [PMC free article] [PubMed] [Google Scholar]
  15. Guilbert L., Robertson S. A., Wegmann T. G. The trophoblast as an integral component of a macrophage-cytokine network. Immunol Cell Biol. 1993 Feb;71(Pt 1):49–57. doi: 10.1038/icb.1993.5. [DOI] [PubMed] [Google Scholar]
  16. Haas L., Wohlsein P., Trautwein G., Stolze B., Kaaden O. R. Violet mink develop an acute disease after experimental infection with Aleutian disease virus (ADV) isolate ADV SL3. Zentralbl Veterinarmed B. 1990 Mar;37(2):106–117. doi: 10.1111/j.1439-0450.1990.tb01033.x. [DOI] [PubMed] [Google Scholar]
  17. Hadlow W. J., Race R. E., Kennedy R. C. Comparative pathogenicity of four strains of Aleutian disease virus for pastel and sapphire mink. Infect Immun. 1983 Sep;41(3):1016–1023. doi: 10.1128/iai.41.3.1016-1023.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hansen M., Lund E. Pregnancy rate and foetal mortality in Aleutian disease virus infected mink. Acta Vet Scand. 1988;29(2):271–272. doi: 10.1186/BF03548384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hassam S., Briner J., Tratschin J. D., Siegl G., Heitz P. U. In situ hybridization for the detection of human parvovirus B19 nucleic acid sequences in paraffin-embedded specimens. Virchows Arch B Cell Pathol Incl Mol Pathol. 1990;59(5):257–261. doi: 10.1007/BF02899412. [DOI] [PubMed] [Google Scholar]
  20. Kaaden O. R., Haas L., Löchelt M., Roth S. Replication of Aleutian disease virus in mink lymphocytes infected in vitro. Intervirology. 1986;25(4):201–209. doi: 10.1159/000149676. [DOI] [PubMed] [Google Scholar]
  21. Kanno H., Wolfinbarger J. B., Bloom M. E. Identification of Aleutian mink disease parvovirus transcripts in macrophages of infected adult mink. J Virol. 1992 Sep;66(9):5305–5312. doi: 10.1128/jvi.66.9.5305-5312.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kilham L., Margolis G., Colby E. D. Cerebellar ataxia and its congenital transmission in cats by feline panleukopenia virus. J Am Vet Med Assoc. 1971 Mar 15;158(6 Suppl):888+–888+. [PubMed] [Google Scholar]
  23. Leiser R., Koob B. Development and characteristics of placentation in a carnivore, the domestic cat. J Exp Zool. 1993 Sep 1;266(6):642–656. doi: 10.1002/jez.1402660612. [DOI] [PubMed] [Google Scholar]
  24. Mengeling W. L., Cutlip R. C. Reproductive disease experimentally induced by exposing pregnant gilts to porcine parvovirus. Am J Vet Res. 1976 Dec;37(12):1393–1400. [PubMed] [Google Scholar]
  25. Mengeling W. L. Prevalence of porcine parvovirus-induced reproductive failure: an abattoir study. J Am Vet Med Assoc. 1978 Jun 1;172(11):1291–1294. [PubMed] [Google Scholar]
  26. Mori S., Wolfinbarger J. B., Miyazawa M., Bloom M. E. Replication of Aleutian mink disease parvovirus in lymphoid tissues of adult mink: involvement of follicular dendritic cells and macrophages. J Virol. 1991 Feb;65(2):952–956. doi: 10.1128/jvi.65.2.952-956.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Padgett G. A., Gorham J. R., Henson J. B. Epizootiologic studies of Aleutian disease. I. Transplacental transmission of the virus. J Infect Dis. 1967 Feb;117(1):35–38. doi: 10.1093/infdis/117.1.35. [DOI] [PubMed] [Google Scholar]
  28. Porter D. D., Larsen A. E., Porter H. G. Aleutian disease of mink. Adv Immunol. 1980;29:261–286. doi: 10.1016/s0065-2776(08)60046-2. [DOI] [PubMed] [Google Scholar]
  29. Porter D. D., Larsen A. E., Porter H. G. Reduced severity of lesions in mink infected transplacentally with Aleutian disease virus. J Immunol. 1977 Sep;119(3):872–876. [PubMed] [Google Scholar]
  30. Porter D. D., Larsen A. E., Porter H. G. The pathogenesis of Aleutian disease of mink. 3. Immune complex arteritis. Am J Pathol. 1973 May;71(2):331–344. [PMC free article] [PubMed] [Google Scholar]
  31. Porter D. D., Larsen A. E., Porter H. G. The pathogenesis of Aleutian disease of mink. I. In vivo viral replication and the host antibody response to viral antigen. J Exp Med. 1969 Sep 1;130(3):575–593. doi: 10.1084/jem.130.3.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Sundqvist C., Amador A. G., Bartke A. Reproduction and fertility in the mink (Mustela vision). J Reprod Fertil. 1989 Mar;85(2):413–441. doi: 10.1530/jrf.0.0850413. [DOI] [PubMed] [Google Scholar]
  33. Takahashi K., Naito M., Katabuchi H., Higashi K. Development, differentiation, and maturation of macrophages in the chorionic villi of mouse placenta with special reference to the origin of Hofbauer cells. J Leukoc Biol. 1991 Jul;50(1):57–68. doi: 10.1002/jlb.50.1.57. [DOI] [PubMed] [Google Scholar]
  34. Trautwein G. Untersuchungen über die Pathogenese der Aleutenkrankheit der Nerze. 3. Pathomorphologie der Organveränderungen. Zentralbl Veterinarmed B. 1970 Feb;17(2):262–285. [PubMed] [Google Scholar]
  35. Uttenthal A., Larsen S., Lund E., Bloom M. E., Storgård T., Alexandersen S. Analysis of experimental mink enteritis virus infection in mink: in situ hybridization, serology, and histopathology. J Virol. 1990 Jun;64(6):2768–2779. doi: 10.1128/jvi.64.6.2768-2779.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Viuff B., Aasted B., Alexandersen S. Role of alveolar type II cells and of surfactant-associated protein C mRNA levels in the pathogenesis of respiratory distress in mink kits infected with Aleutian mink disease parvovirus. J Virol. 1994 Apr;68(4):2720–2725. doi: 10.1128/jvi.68.4.2720-2725.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

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