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. 1987 Oct;129(1):9–13.

The third component of complement is transcribed and secreted by cultured human endothelial cells.

H B Warren 1, P Pantazis 1, P F Davies 1
PMCID: PMC1899691  PMID: 2444110

Abstract

Monotypic cultures of passaged human umbilical vein endothelial cells secrete C3 protein, which is specifically immunoprecipitable from cell lysates and conditioned medium. A transcript migrating at 5.3 kilobases hybridizes with a mouse-derived C3 DNA probe in parallel cultures of endothelial cells, hepatoma cells (HepG2), and freshly isolated human monocytes. Steady-state transcript levels were as follows: HepG2 cells, 1.0; monocytes, 0.05; endothelial cells, 0.01. These data suggest that C3, an indispensible component for activation of complement by both the classical and alternative pathways, is locally synthesized in the vascular bed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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