Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1987 Oct;129(1):74–85.

Extracellular matrix synthesis by undifferentiated childhood tumor cell lines.

S Scarpa 1, A Modesti 1, T J Triche 1
PMCID: PMC1899707  PMID: 2821816

Abstract

The authors have examined extracellular matrix (ECM) biosynthesis by small round cell tumors of childhood. Basal lamina (laminin and Type IV collagen) and stroma (collagens I, III, and V and fibronectin) constituents were studied. It was found that these tumors synthesize ECM in characteristic patterns. Five Ewing's sarcomas variably synthesized small amounts of all ECM constituents except Type V collagen. All eight neural tumors (neuroblastoma and primitive neural tumors) synthesized fibronectin (unlike some Ewing's sarcomas), as well as laminin and Type IV collagen (2 cases lacked Type IV collagen synthesis). No stromal (I/III) collagen synthesis was observed by neural tumors. All soft tissue sarcomas except an embryonal rhabdomyosarcoma synthesized stromal collagens and often laminin or fibronectin as well. Lymphomas synthesized no ECM of any kind. The synthesis of stromal collagens by sarcomas but not neural tumors serves to distinguish these two tumor types, especially Ewing's sarcoma from neuroblastoma. The presence of any ECM synthesis excludes lymphoma from diagnostic consideration.

Full text

PDF
74

Images in this article

77Figure 1
on p.77
78Figure 2
on p.78
79Figure 3
on p.79
79Figure 4
on p.79
80Figure 5
on p.80
80Figure 6
on p.80
81Figure 7
on p.81
81Figure 8
on p.81
81Figure 9
on p.81
82Figure 10
on p.82
82Figure 11
on p.82

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alitalo K., Keski-Oja J., Vaheri A. Extracellular matrix proteins characterize human tumor cell lines. Int J Cancer. 1981 Jun 15;27(6):755–761. doi: 10.1002/ijc.2910270605. [DOI] [PubMed] [Google Scholar]
  2. Alitalo K., Kurkinen M., Vaheri A., Virtanen I., Rohde H., Timpl R. Basal lamina glycoproteins are produced by neuroblastoma cells. Nature. 1980 Oct 2;287(5781):465–466. doi: 10.1038/287465a0. [DOI] [PubMed] [Google Scholar]
  3. Alitalo K., Kurkinen M., Virtanen I., Mellström K., Vaheri A. Deposition of basement membrane proteins in attachment and neurite formation of cultured murine C-1300 neuroblastoma cells. J Cell Biochem. 1982;18(1):25–35. doi: 10.1002/jcb.1982.240180104. [DOI] [PubMed] [Google Scholar]
  4. Alitalo K., Myllyla R., Sage H., Pritzl P., Vaheri A., Bornstein P. Biosynthesis of type V procollagen by A204, a human rhabdomyosarcoma cell line. J Biol Chem. 1982 Aug 10;257(15):9016–9024. [PubMed] [Google Scholar]
  5. Autio-Harmainen H., Apaja-Sarkkinen M., Martikainen J., Taipale A., Rapola J. Production of basement membrane laminin and type IV collagen by tumors of striated muscle: an immunohistochemical study of rhabdomyosarcomas of different histologic types and a benign vaginal rhabdomyoma. Hum Pathol. 1986 Dec;17(12):1218–1224. doi: 10.1016/s0046-8177(86)80563-9. [DOI] [PubMed] [Google Scholar]
  6. Bailey A. J., Shellswell G. B., Duance V. C. Identification and change of collagen types in differentiating myoblasts and developing chick muscle. Nature. 1979 Mar 1;278(5699):67–69. doi: 10.1038/278067a0. [DOI] [PubMed] [Google Scholar]
  7. Biedler J. L., Helson L., Spengler B. A. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 1973 Nov;33(11):2643–2652. [PubMed] [Google Scholar]
  8. Bonadio J. F., Sage H., Cheng F., Bernstein J., Striker G. E. Localization of collagen types IV and V, laminin, and heparan sulfate proteoglycan to the basal lamina of kidney epithelial cells in transfilter metanephric culture. Am J Pathol. 1984 Aug;116(2):289–296. [PMC free article] [PubMed] [Google Scholar]
  9. Cavazzana A. O., Miser J. S., Jefferson J., Triche T. J. Experimental evidence for a neural origin of Ewing's sarcoma of bone. Am J Pathol. 1987 Jun;127(3):507–518. [PMC free article] [PubMed] [Google Scholar]
  10. DeClerck Y. A., Bogenmann E., Jones P. A. Collagen synthesis by short-term explants of pediatric tumors. Cancer Res. 1985 Mar;45(3):1229–1238. [PubMed] [Google Scholar]
  11. Dickman P. S., Liotta L. A., Triche T. J. Ewing's sarcoma. Characterization in established cultures and evidence of its histogenesis. Lab Invest. 1982 Oct;47(4):375–382. [PubMed] [Google Scholar]
  12. Giard D. J., Aaronson S. A., Todaro G. J., Arnstein P., Kersey J. H., Dosik H., Parks W. P. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst. 1973 Nov;51(5):1417–1423. doi: 10.1093/jnci/51.5.1417. [DOI] [PubMed] [Google Scholar]
  13. Israel M. A., Thiele C., Whang-Peng J., Kao-Shan C. S., Triche T. J., Miser J. Peripheral neuroepithelioma: genetic analysis of tumor derived cell lines. Prog Clin Biol Res. 1985;175:161–170. [PubMed] [Google Scholar]
  14. Jaffe R., Santamaria M., Yunis E. J., Tannery N. H., Agostini R. M., Jr, Medina J., Goodman M. The neuroectodermal tumor of bone. Am J Surg Pathol. 1984 Dec;8(12):885–898. doi: 10.1097/00000478-198412000-00001. [DOI] [PubMed] [Google Scholar]
  15. Krieg T., Timpl R., Alitalo K., Kurkinen M., Vaheri A. Type III procollagen is the major collageneous component produced by a continuous rhabdomyosarcoma cell line. FEBS Lett. 1979 Aug 15;104(2):405–409. doi: 10.1016/0014-5793(79)80863-7. [DOI] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Lanzer W. L., Liotta L. A., Yee C., Azar H. A., Costa J. Synthesis of pro-collagen type II by a xenotransplanted human chondroblastic osteosarcoma. Am J Pathol. 1981 Sep;104(3):217–226. [PMC free article] [PubMed] [Google Scholar]
  18. Linsenmayer T. F., Trelstad R. L., Gross J. The collagen of chick embryonic notochord. Biochem Biophys Res Commun. 1973 Jul 2;53(1):39–45. doi: 10.1016/0006-291x(73)91397-1. [DOI] [PubMed] [Google Scholar]
  19. Liotta L. A., Foidart J. M., Robey P. G., Martin G. R., Gullino P. M. Identification of micrometastasis of breast carcinomas by presence of basement membrane collagen. Lancet. 1979 Jul 21;2(8134):146–147. doi: 10.1016/s0140-6736(79)90024-2. [DOI] [PubMed] [Google Scholar]
  20. Liotta L. A., Wicha M. S., Foidart J. M., Rennard S. I., Garbisa S., Kidwell W. R. Hormonal requirements for basement membrane collagen deposition by cultured rat mammary epithelium. Lab Invest. 1979 Dec;41(6):511–518. [PubMed] [Google Scholar]
  21. McAllister R. M., Melnyk J., Finkelstein J. Z., Adams E. C., Jr, Gardner M. B. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer. 1969 Sep;24(3):520–526. doi: 10.1002/1097-0142(196909)24:3<520::aid-cncr2820240313>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  22. Minowada J., Onuma T., Moore G. E. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst. 1972 Sep;49(3):891–895. [PubMed] [Google Scholar]
  23. Modesti A., Kalebic T., Scarpa S., Togo S., Grotendorst G., Liotta L. A., Triche T. J. Type V collagen in human amnion is a 12 nm fibrillar component of the pericellular interstitium. Eur J Cell Biol. 1984 Nov;35(2):246–255. [PubMed] [Google Scholar]
  24. PATTON R. B., HORN R. C., Jr Rhabdomyosarcoma: clinical and pathological features and comparison with human fetal and embryonal skeletal muscle. Surgery. 1962 Oct;52:572–584. [PubMed] [Google Scholar]
  25. Reynolds C. P., Frenkel E. P., Smith R. G. Growth characteristics of neuroblastoma in vitro correlate with patient survival. Trans Assoc Am Physicians. 1980;93:203–211. [PubMed] [Google Scholar]
  26. Ross R. A., Spengler B. A., Biedler J. L. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J Natl Cancer Inst. 1983 Oct;71(4):741–747. [PubMed] [Google Scholar]
  27. Sage H., Balian G., Vogel A. M., Bornstein P. Type VIII collagen. Synthesis by normal and malignant cells in culture. Lab Invest. 1984 Feb;50(2):219–231. [PubMed] [Google Scholar]
  28. Sariola H., Ekblom P., Rapola J., Vaheri A., Timpl R. Extracellular matrix and epithelial differentiation of Wilms' tumor. Am J Pathol. 1985 Jan;118(1):96–107. [PMC free article] [PubMed] [Google Scholar]
  29. Sasse J., von der Mark H., Kühl U., Dessau W., von der Mark K. Origin of collagen types I, III, and V in cultures of avian skeletal muscle. Dev Biol. 1981 Apr 15;83(1):79–89. doi: 10.1016/s0012-1606(81)80010-3. [DOI] [PubMed] [Google Scholar]
  30. Schlesinger H. R., Gerson J. M., Moorhead P. S., Maguire H., Hummeler K. Establishment and characterization of human neuroblastoma cell lines. Cancer Res. 1976 Sep;36(9 PT1):3094–3100. [PubMed] [Google Scholar]
  31. Stern R. Current concepts in the diagnosis of human soft tissue sarcomas. Hum Pathol. 1981 Sep;12(9):777–781. doi: 10.1016/s0046-8177(81)80080-9. [DOI] [PubMed] [Google Scholar]
  32. Stern R., Wilczek J., Thorpe W. P., Rosenberg S. A., Cannon G. Procollagens as markers for the cell of origin of human bone tumors. Cancer Res. 1980 Feb;40(2):325–328. [PubMed] [Google Scholar]
  33. Trelstad R. L., Kang A. H., Cohen A. M., Hay E. D. Collagen synthesis in vitro by embryonic spinal cord epithelium. Science. 1973 Jan 19;179(4070):295–297. doi: 10.1126/science.179.4070.295. [DOI] [PubMed] [Google Scholar]
  34. Triche T. J., Askin F. B. Neuroblastoma and the differential diagnosis of small-, round-, blue-cell tumors. Hum Pathol. 1983 Jul;14(7):569–595. doi: 10.1016/s0046-8177(83)80202-0. [DOI] [PubMed] [Google Scholar]
  35. Tumilowicz J. J., Nichols W. W., Cholon J. J., Greene A. E. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 1970 Aug;30(8):2110–2118. [PubMed] [Google Scholar]
  36. Whang-Peng J., Triche T. J., Knutsen T., Miser J., Douglass E. C., Israel M. A. Chromosome translocation in peripheral neuroepithelioma. N Engl J Med. 1984 Aug 30;311(9):584–585. doi: 10.1056/NEJM198408303110907. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES