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. 1996 Mar;70(3):1898–1904. doi: 10.1128/jvi.70.3.1898-1904.1996

The herpes simplex virus type 1 VP5 promoter contains a cis-acting element near the cap site which interacts with a cellular protein.

C J Huang 1, M D Petroski 1, N T Pande 1, M K Rice 1, E K Wagner 1
PMCID: PMC190018  PMID: 8627715

Abstract

The promoter controlling the expression of the transcript encoding the major herpes simplex virus type 1 capsid protein (VP5, UL19) extends only 60 bases or so from a functional Sp1 site at --48 to include a cis-acting element 3' of the transcript start site. In the present communication, we report the generation of recombinant viruses bearing mutations between --6 and + 8 relative to the cap site in the VP5 promoter controlling expression of a reporter gene. Analysis of the effects of these mutations upon reporter gene expression along with the results of protein binding assays demonstrates that this cap transcription element functionally interacts with a cellular protein of a normal size of 40 kDa. Thus, like the strict late UL38 promoter characterized earlier, the late VP5 promoter has the essential properties of a cellular promoter.

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Selected References

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  1. Boyer T. G., Maquat L. E. Minimal sequence and factor requirements for the initiation of transcription from an atypical, TATATAA box-containing housekeeping promoter. J Biol Chem. 1990 Nov 25;265(33):20524–20532. [PubMed] [Google Scholar]
  2. Brunelle A., Schleif R. F. Missing contact probing of DNA-protein interactions. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6673–6676. doi: 10.1073/pnas.84.19.6673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goodart S. A., Guzowski J. F., Rice M. K., Wagner E. K. Effect of genomic location on expression of beta-galactosidase mRNA controlled by the herpes simplex virus type 1 UL38 promoter. J Virol. 1992 May;66(5):2973–2981. doi: 10.1128/jvi.66.5.2973-2981.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Guzowski J. F., Singh J., Wagner E. K. Transcriptional activation of the herpes simplex virus type 1 UL38 promoter conferred by the cis-acting downstream activation sequence is mediated by a cellular transcription factor. J Virol. 1994 Dec;68(12):7774–7789. doi: 10.1128/jvi.68.12.7774-7789.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guzowski J. F., Wagner E. K. Mutational analysis of the herpes simplex virus type 1 strict late UL38 promoter/leader reveals two regions critical in transcriptional regulation. J Virol. 1993 Sep;67(9):5098–5108. doi: 10.1128/jvi.67.9.5098-5108.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hardy W. R., Sandri-Goldin R. M. Herpes simplex virus inhibits host cell splicing, and regulatory protein ICP27 is required for this effect. J Virol. 1994 Dec;68(12):7790–7799. doi: 10.1128/jvi.68.12.7790-7799.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huang C. J., Rice M. K., Devi-Rao G. B., Wagner E. K. The activity of the pseudorabies virus latency-associated transcript promoter is dependent on its genomic location in herpes simplex virus recombinants as well as on the type of cell infected. J Virol. 1994 Mar;68(3):1972–1976. doi: 10.1128/jvi.68.3.1972-1976.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huang C. J., Wagner E. K. The herpes simplex virus type 1 major capsid protein (VP5-UL19) promoter contains two cis-acting elements influencing late expression. J Virol. 1994 Sep;68(9):5738–5747. doi: 10.1128/jvi.68.9.5738-5747.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kibler P. K., Duncan J., Keith B. D., Hupel T., Smiley J. R. Regulation of herpes simplex virus true late gene expression: sequences downstream from the US11 TATA box inhibit expression from an unreplicated template. J Virol. 1991 Dec;65(12):6749–6760. doi: 10.1128/jvi.65.12.6749-6760.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  11. Sandri-Goldin R. M., Mendoza G. E. A herpesvirus regulatory protein appears to act post-transcriptionally by affecting mRNA processing. Genes Dev. 1992 May;6(5):848–863. doi: 10.1101/gad.6.5.848. [DOI] [PubMed] [Google Scholar]
  12. Wagner E. K., Guzowski J. F., Singh J. Transcription of the herpes simplex virus genome during productive and latent infection. Prog Nucleic Acid Res Mol Biol. 1995;51:123–165. doi: 10.1016/s0079-6603(08)60878-8. [DOI] [PubMed] [Google Scholar]
  13. Weis L., Reinberg D. Transcription by RNA polymerase II: initiator-directed formation of transcription-competent complexes. FASEB J. 1992 Nov;6(14):3300–3309. doi: 10.1096/fasebj.6.14.1426767. [DOI] [PubMed] [Google Scholar]
  14. Wilson A. C., Cleary M. A., Lai J. S., LaMarco K., Peterson M. G., Herr W. Combinatorial control of transcription: the herpes simplex virus VP16-induced complex. Cold Spring Harb Symp Quant Biol. 1993;58:167–178. doi: 10.1101/sqb.1993.058.01.021. [DOI] [PubMed] [Google Scholar]
  15. Zhang Y. F., Devi-Rao G. B., Rice M., Sandri-Goldin R. M., Wagner E. K. The effect of elevated levels of herpes simplex virus alpha-gene products on the expression of model early and late genes in vivo. Virology. 1987 Mar;157(1):99–106. doi: 10.1016/0042-6822(87)90318-7. [DOI] [PubMed] [Google Scholar]
  16. Zhang Y. F., Wagner E. K. The kinetics of expression of individual herpes simplex virus type 1 transcripts. Virus Genes. 1987 Nov;1(1):49–60. doi: 10.1007/BF00125685. [DOI] [PubMed] [Google Scholar]

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