Comparative growth dynamics and morphology between cultured myofibroblasts from granulating wounds and dermal fibroblasts.

Abstract

Rat myofibroblasts from granulating wound biopsies (RGW) were successfully cultured and compared in terms of growth and morphology with fibroblasts from uninjured rat dermis (RD). Populations of early passage (P-3) RGW myofibroblasts grew significantly more slowly than RD fibroblasts. Logarithmic growth was nearly the same in late passage (P-30) populations of both cell types. Early passage RGW myofibroblasts were similar to those in vivo, as shown by well-defined microfilament bundles. RD fibroblasts contained less well defined microfilaments. Both late passage RGW myofibroblasts and RD fibroblasts displayed evidence of morphologic dedifferentiation. These data show that morphologic features of myofibroblasts and fibroblasts in vivo are maintained in vitro. Evidence is presented that cultured animal myofibroblasts maintain differentiation in early passage, whereas late passage cells suggest that these differences disappear with time.

Full text

PDF

• 187
• 188
• 189
• 190
• 191
• …
• 200
• Next

Images in this article

Figure 2
on p.189

Figure 3
on p.190

Figure 4
on p.190

Figure 5
on p.191

Figure 6
on p.192

Figure 7
on p.193

Figure 8
on p.194

Figure 9
on p.195

Figure 10
on p.196

Figure 11
on p.196

Figure 12
on p.197

Figure 13
on p.197

Figure 14
on p.198

Figure 15
on p.199