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. 1996 Mar;70(3):2027–2031. doi: 10.1128/jvi.70.3.2027-2031.1996

Mutational analysis of the mengovirus poly(C) tract and surrounding heteropolymeric sequences.

L R Martin 1, G M Duke 1, J E Osorio 1, D J Hall 1, A C Palmenberg 1
PMCID: PMC190034  PMID: 8627731

Abstract

Previously, we described three mengovirus mutants derived from cDNA plasmids, containing shortened poly(C) tracts (C8, C12, and C13UC10), that exhibited strong attenuation for virulence in mice yet grew like wild-type virus in HeLa cells. Thirteen additional mutants hav now been constructed and characterized. Five of these differ only in poly(C) length, including one with a precise deletion of the tract. The other mutants bear deletions into the regions juxtaposing poly(C). Studies with HeLa cells confirm the essential dispensability of mengovirus's poly(C) tract but reveal a subtle, measurable correlation between poly(C) length and plaque diameter. Virulence studies with mice also revealed a strong correlation between poly(C) length and virulence. For the poly(C)-flanking mutations, the 15 bases directly 5' of the tract proved dispensable for virus viability, whereas the 20 to 30 bases 3' of poly(C) were critical for growth, thus implicating this region in the basal replication of the virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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