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. 1984 Nov;117(2):316–325.

Dog thyroid glands after chronic administration of antithyroid drugs.

Y Kameda
PMCID: PMC1900447  PMID: 6208786

Abstract

The effect of chronic administration of antithyroid drugs on the composition of thyroid proteins in dogs was investigated. After administration of thiourea for periods of 10 days to 4 months and also of ethylenethiourea for 6 months thyroid glands were examined morphologically by immunoperoxidase staining using anti-19S-thyroglobulin and anti-albumin antisera. After follicles were devoid of apparent colloid, the immunoreactivity of follicular cells to the 19S antiserum progressively increased with administration periods. A large number of follicular cells were also densely stained with the albumin antiserum. In contrast to the immunoreaction of 19S-thyroglobulin, which was detected in all follicular cells, the appearance of immunoreactive albumin was restricted to 60-80% of the follicular cell population even after several months' administration. Thyroid extracts after treatment with ethylenethiourea for 6 months were analyzed with gel chromatography, polyacrylamide gradient gel electrophoresis, and immunoblotting. Large-molecular-weight components, including 27S iodoprotein, were completely absent in the goitrous thyroids. On the contrary, small-molecular-weight components markedly increased in number and amount, although of these two or three bands alone were weakly immunoreactive to the 19S antiserum. Both 19S and 12S components were still significant products, showing an intense immunoreactivity for 19S-thyroglobulin after the chronic administration of goitrogen. Albumin markedly increased in amount and constituted 24.2% +/- 7.6% of total stained proteins, seven times as high as the value of normal controls (3.4% +/- 2.3%).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg G., Björkman U. The structure and properties of 27S and larger iodoproteins in the thyroid gland. Biochim Biophys Acta. 1975 Sep 9;405(1):11–22. doi: 10.1016/0005-2795(75)90309-8. [DOI] [PubMed] [Google Scholar]
  2. Cooper D. S., Kieffer J. D., Halpern R., Saxe V., Mover H., Maloof F., Ridgway E. C. Propylthiouracil (PTU) pharmacology in the rat. II. Effects of PTU on thyroid function. Endocrinology. 1983 Sep;113(3):921–928. doi: 10.1210/endo-113-3-921. [DOI] [PubMed] [Google Scholar]
  3. Davidson B., Soodak M., Neary J. T., Strout H. V., Kieffer J. D., Mover H., Maloof F. The irreversible inactivation of thyroid peroxidase by methylmercaptoimidazole, thiouracil, and propylthiouracil in vitro and its relationship to in vivo findings. Endocrinology. 1978 Sep;103(3):871–882. doi: 10.1210/endo-103-3-871. [DOI] [PubMed] [Google Scholar]
  4. Falconer I. R., Roitt I. M., Seamark R. F., Torrigiani G. Studies on the congenitally goitrous sheep. Iodoproteins of the goitre. Biochem J. 1970 Apr;117(3):417–424. doi: 10.1042/bj1170417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Feldmann G., Penaud-Laurencin J., Crassous J., Benhamou J. P. Albumin synthesis by human liver cells: its morphological demonstration. Gastroenterology. 1972 Dec;63(6):1036–1048. [PubMed] [Google Scholar]
  6. Frati L., Bilstad J., Edelhoch H., Rall J. E., Salvatore G. Biosynthesis of the 27 S thyroid iodoprotein. Arch Biochem Biophys. 1974 May;162(1):126–134. doi: 10.1016/0003-9861(74)90111-8. [DOI] [PubMed] [Google Scholar]
  7. Griessen M., Lemarchand-Béraud T. Thyrotropin secretion and metabolism in rats during propylthiouracil treatment. Endocrinology. 1973 Jan;92(1):166–173. doi: 10.1210/endo-92-1-166. [DOI] [PubMed] [Google Scholar]
  8. Guillouzo A., Belanger L., Beaumont C., Valet J. P., Briggs R., Chiu J. F. Cellular and subcellular immunolocalization of alpha1-fetoprotein and albumin in rat liver. Reevaluation of various experimental conditions. J Histochem Cytochem. 1978 Nov;26(11):948–959. doi: 10.1177/26.11.82574. [DOI] [PubMed] [Google Scholar]
  9. Kameda Y. Degranulation and appearance of vesicular inclusions in canine C cells after administration of antithyroid drug. Cell Tissue Res. 1982;225(2):315–318. doi: 10.1007/BF00214684. [DOI] [PubMed] [Google Scholar]
  10. Kameda Y., Ikeda A. Immunochemical and immunohistochemical studies on the 27 S iodoprotein of dog thyroid with reference to thyroglobulin-like reaction of the parafollicular cells. Biochim Biophys Acta. 1979 Apr 25;577(2):241–247. doi: 10.1016/0005-2795(79)90027-8. [DOI] [PubMed] [Google Scholar]
  11. Kameda Y., Ikeda A. Immunohistochemical study of the C-cell complex of dog thyroid glands with reference to the reactions of calcitonin, C-thyroglobulin and 19S thyroglobulin. Cell Tissue Res. 1980;208(3):405–415. doi: 10.1007/BF00233873. [DOI] [PubMed] [Google Scholar]
  12. Kameda Y., Ikeda A. The identification of a specific fragment of dog thyroglobulin responsible for immunoreactivity to parafollicular cells. Endocrinology. 1978 Jun;102(6):1702–1709. doi: 10.1210/endo-102-6-1702. [DOI] [PubMed] [Google Scholar]
  13. Kameda Y., Shigemoto H., Ikeda A. Development and cytodifferentiation of C cell complexes in dog fetal thyroids. An immunohistochemical study using anti-calcitonin, anti-C-thyroglobulin and anti-19S thyroglobulin antisera. Cell Tissue Res. 1980;206(3):403–415. doi: 10.1007/BF00237970. [DOI] [PubMed] [Google Scholar]
  14. LeBouton A. V., Masse J. P. A random arrangement of albumin-containing hepatocytes seen with histo-immunologic methods. I. Verification of the artifact. Anat Rec. 1980 Jun;197(2):183–194. doi: 10.1002/ar.1091970207. [DOI] [PubMed] [Google Scholar]
  15. Lissitzky S., Bismuth J., Codaccioni J. L., Cartouzou G. Congenital goiter with iodoalbumin replacing thyroglobulin and defect of deiodination of iodotyrosines. Serum origin of the thyroid iodoalbumin. J Clin Endocrinol Metab. 1968 Dec;28(12):1797–1806. doi: 10.1210/jcem-28-12-1797. [DOI] [PubMed] [Google Scholar]
  16. Lissitzky S., Bismuth J., Jaquet P., Castay M., Michel-Béchet M., Koutras D. A., Pharmakiotis A. D., Moschos A., Psarras A., Malamos B. Congenital goiter with impaired thyroglobulin synthesis. J Clin Endocrinol Metab. 1973 Jan;36(1):17–29. doi: 10.1210/jcem-36-1-17. [DOI] [PubMed] [Google Scholar]
  17. Pammenter M., Bester A., Albrecht C., van Jaarsveld P. Afrikander cattle congenital goiter: characteristics of the defective 19S thyroglobulin synthesis. Endocrinology. 1979 Jun;104(6):1853–1861. doi: 10.1210/endo-104-6-1853. [DOI] [PubMed] [Google Scholar]
  18. Robbins J., Van Zyl A., Van der Walt K. Abnormal thyroglobin in congenital goiter of cattle. Endocrinology. 1966 Jun;78(6):1213–1223. doi: 10.1210/endo-78-6-1213. [DOI] [PubMed] [Google Scholar]
  19. SALVATORE G., VECCHIO G., SALVATORE M., CAHNMANN H. J., ROBBINS J. 27S THYROID IODOPROTEIN. ISOLATION AND PROPERTIES. J Biol Chem. 1965 Jul;240:2935–2943. [PubMed] [Google Scholar]
  20. SEED R. W., GOLDBERG I. H. BIOSYNTHESIS OF THYROGLOBULIN. II. ROLE OF SUBUNITS, IODINATION, AND RIBONUCLEIC ACID SYNTHESIS. J Biol Chem. 1965 Feb;240:764–773. [PubMed] [Google Scholar]
  21. Smeds S., Anderberg B. Change of the protein composition of the thyroid colloid during treatment with propylthiouracil and thyroxine: a microgel electrophoretic study of single rat thyroid follicles. Biochim Biophys Acta. 1978 Aug 3;542(1):47–55. doi: 10.1016/0304-4165(78)90231-3. [DOI] [PubMed] [Google Scholar]
  22. Strum J. M., Karnovsky M. J. Aminotriazole goiter. Fine structure and localization of thyroid peroxidase activity. Lab Invest. 1971 Jan;24(1):1–12. [PubMed] [Google Scholar]
  23. Taurog A. The mechanism of action of the thioureylene antithyroid drugs. Endocrinology. 1976 Apr;98(4):1031–1046. doi: 10.1210/endo-98-4-1031. [DOI] [PubMed] [Google Scholar]
  24. Thomas-Morvan C. Iodothyronine content of human thyroid albumin. Acta Endocrinol (Copenh) 1976 Feb;81(2):288–297. doi: 10.1530/acta.0.0810288. [DOI] [PubMed] [Google Scholar]
  25. Thompson J. A., Goldberg I. H. Biosynthesis of thyroglobulin and its subunits in vivo in the rat thyroid gland. Endocrinology. 1968 Apr;82(4):805–817. doi: 10.1210/endo-82-4-805. [DOI] [PubMed] [Google Scholar]
  26. Thomson J. A., Bissett J. M. Further studies on the 27S and 32S optical density peaks of the rat thyroid. Acta Endocrinol (Copenh) 1970 Sep;65(1):175–183. doi: 10.1530/acta.0.0650175. [DOI] [PubMed] [Google Scholar]
  27. Torresani J., Roques M., Peyrot A., Lissitzky S. Mise en evidence, purification et propriétés d'une iodoalbumine, constituant physiologique de la glande thyroide de rat. Acta Endocrinol (Copenh) 1968 Jan;57(1):153–167. [PubMed] [Google Scholar]
  28. Van der Walt B., Van Jaarsveld P. Bovine 37S iodoprotein: isolation and characterization. Arch Biochem Biophys. 1972 Jun;150(2):786–791. doi: 10.1016/0003-9861(72)90099-9. [DOI] [PubMed] [Google Scholar]
  29. Yokota S., Fahimi H. D. Immunocytochemical localization of albumin in the secretory apparatus of rat liver parenchymal cells. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4970–4974. doi: 10.1073/pnas.78.8.4970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. van Voorthuizen W. F., de Vijlder J. J., van Dijk J. E., Tegelaers W. H. Euthyroidism via iodide supplementation in hereditary congenital goiter with thyroglobulin deficiency. Endocrinology. 1978 Dec;103(6):2105–2111. doi: 10.1210/endo-103-6-2105. [DOI] [PubMed] [Google Scholar]

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