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. 1984 Sep;116(3):363–369.

Cytokeratin and neurofilament in lung carcinomas.

G N van Muijen, D J Ruiter, C van Leeuwen, F A Prins, K Rietsema, S O Warnaar
PMCID: PMC1900469  PMID: 6206727

Abstract

Three monoclonal antibodies, one directed against cytokeratin (clone 80) and two directed against neurofilament (clones 2F11 and 3G6), were used in the study of a series of 77 lung carcinomas by immunohistochemical staining. The anti-cytokeratin antibody, a very broadly reacting antibody directed against an antigenic determinant common to a great number of cytokeratins, was applicable on frozen sections. The two anti-neurofilament antibodies, directed against the 70 kD protein (clone 2F11) and the 160 kD and 200 kD proteins (clone 3G6) of neurofilament, were applicable on both frozen sections and paraffin sections. The staining results on the lung carcinomas indicate that all types of tumors studied, including small-cell anaplastic carcinoma, are markedly positive for cytokeratin. Frozen sections of five and formalin-fixed and paraffin-embedded sections of six other small-cell anaplastic carcinomas were negative with both anti-neurofilament monoclonal antibodies. One poorly differentiated squamous cell carcinoma positive with anti-neurofilament clone 2F11 but negative with clone 3G6. This distribution of cytoskeletal proteins demonstrates the epithelial differentiation of all types of lung carcinomas. Neuroendocrine differentiation of lung carcinomas as found in the small-cell anaplastic types does not result in expression of neurofilament proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abeloff M. D., Eggleston J. C., Mendelsohn G., Ettinger D. S., Baylin S. B. Changes in morphologic and biochemical characteristics of small cell carcinoma of the lung. A clinicopathologic study. Am J Med. 1979 May;66(5):757–764. doi: 10.1016/0002-9343(79)91113-6. [DOI] [PubMed] [Google Scholar]
  2. Altmannsberger M., Weber K., Hölscher A., Schauer A., Osborn M. Antibodies to intermediate filaments as diagnostic tools: human gastrointestinal carcinomas express prekeratin. Lab Invest. 1982 May;46(5):520–526. [PubMed] [Google Scholar]
  3. Berger C. L., Goodwin G., Mendelsohn G., Eggleston J. C., Abeloff M. D., Aisner S., Baylin S. B. Endocrine-related biochemistry in the spectrum of human lung carcinoma. J Clin Endocrinol Metab. 1981 Aug;53(2):422–429. doi: 10.1210/jcem-53-2-422. [DOI] [PubMed] [Google Scholar]
  4. Caselitz J., Osborn M., Wustrow J., Seifert G., Weber K. The expression of different intermediate-sized filaments in human salivary glands and their tumours. Pathol Res Pract. 1982;175(2-3):266–278. doi: 10.1016/S0344-0338(82)80113-1. [DOI] [PubMed] [Google Scholar]
  5. Dahl D., Bignami A. Preparation of antisera to neurofilament protein from chicken brain and human sciatic nerve. J Comp Neurol. 1977 Dec 15;176(4):645–657. doi: 10.1002/cne.901760412. [DOI] [PubMed] [Google Scholar]
  6. Dräger U. C. Coexistence of neurofilaments and vimentin in a neurone of adult mouse retina. Nature. 1983 May 12;303(5913):169–172. doi: 10.1038/303169a0. [DOI] [PubMed] [Google Scholar]
  7. Franke W. W., Schmid E., Osborn M., Weber K. Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5034–5038. doi: 10.1073/pnas.75.10.5034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Franke W. W., Schmid E., Osborn M., Weber K. Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5034–5038. doi: 10.1073/pnas.75.10.5034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gazdar A. F., Carney D. N., Russell E. K., Sims H. L., Baylin S. B., Bunn P. A., Jr, Guccion J. G., Minna J. D. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 1980 Oct;40(10):3502–3507. [PubMed] [Google Scholar]
  10. Hattori S., Matsuda M., Tateishi R., Nishihara H., Horai T. Oat-cell carcinoma of the lung. Clinical and morphological studies in relation to its histogenesis. Cancer. 1972 Oct;30(4):1014–1024. doi: 10.1002/1097-0142(197210)30:4<1014::aid-cncr2820300424>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
  11. Krepler R., Denk H., Artlieb U., Fichtinger E., Davidovits A. Antibodies to intermediate filament proteins as molecular markers in clinical tumor pathology. Differentiation of carcinomas by their reaction with different cytokeratin antibodies. Pathol Res Pract. 1982;175(2-3):212–226. doi: 10.1016/S0344-0338(82)80109-X. [DOI] [PubMed] [Google Scholar]
  12. Lane E. B., Goodman S. L., Trejdosiewicz L. K. Disruption of the keratin filament network during epithelial cell division. EMBO J. 1982;1(11):1365–1372. doi: 10.1002/j.1460-2075.1982.tb01324.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lane E. B., Hogan B. L., Kurkinen M., Garrels J. I. Co-expression of vimentin and cytokeratins in parietal endoderm cells of early mouse embryo. Nature. 1983 Jun 23;303(5919):701–704. doi: 10.1038/303701a0. [DOI] [PubMed] [Google Scholar]
  14. Lehto V. P., Stenman S., Miettinen M., Dahl D., Virtanen I. Expression of a neural type of intermediate filament as a distinguishing feature between oat cell carcinoma and other lung cancers. Am J Pathol. 1983 Feb;110(2):113–118. [PMC free article] [PubMed] [Google Scholar]
  15. Liem R. Simultaneous separation and purification of neurofilament and glial filament proteins from brain. J Neurochem. 1982 Jan;38(1):142–150. doi: 10.1111/j.1471-4159.1982.tb10865.x. [DOI] [PubMed] [Google Scholar]
  16. Nakane P K, Pierce G B., Jr Enzyme-labeled antibodies: preparation and application for the localization of antigens. J Histochem Cytochem. 1966 Dec;14(12):929–931. doi: 10.1177/14.12.929. [DOI] [PubMed] [Google Scholar]
  17. Osborn M., Weber K. Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest. 1983 Apr;48(4):372–394. [PubMed] [Google Scholar]
  18. Ramaekers F. C., Haag D., Kant A., Moesker O., Jap P. H., Vooijs G. P. Coexpression of keratin- and vimentin-type intermediate filaments in human metastatic carcinoma cells. Proc Natl Acad Sci U S A. 1983 May;80(9):2618–2622. doi: 10.1073/pnas.80.9.2618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ramaekers F. C., Osborn M., Schimid E., Weber K., Bloemendal H., Franke W. W. Identification of the cytoskeletal proteins in lens-forming cells, a special epitheloid cell type. Exp Cell Res. 1980 Jun;127(2):309–327. doi: 10.1016/0014-4827(80)90437-1. [DOI] [PubMed] [Google Scholar]
  20. Ramaekers F., Puts J., Kant A., Moesker O., Jap P., Vooijs P. Differential diagnosis of human carcinomas, sarcomas and their metastases using antibodies to intermediate-sized filaments. Eur J Cancer Clin Oncol. 1982 Dec;18(12):1251–1257. doi: 10.1016/0277-5379(82)90126-2. [DOI] [PubMed] [Google Scholar]
  21. Ramaekers F., Puts J., Moesker O., Kant A., Jap P., Vooijs P. Demonstration of keratin in human adenocarcinomas. Am J Pathol. 1983 May;111(2):213–223. [PMC free article] [PubMed] [Google Scholar]
  22. Tapia F. J., Polak J. M., Barbosa A. J., Bloom S. R., Marangos P. J., Dermody C., Pearse A. G. Neuron-specific enolase is produced by neuroendocrine tumours. Lancet. 1981 Apr 11;1(8224):808–811. doi: 10.1016/s0140-6736(81)92682-9. [DOI] [PubMed] [Google Scholar]
  23. The World Health Organization histological typing of lung tumours. Second edition. Am J Clin Pathol. 1982 Feb;77(2):123–136. doi: 10.1093/ajcp/77.2.123. [DOI] [PubMed] [Google Scholar]
  24. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Virtanen I., Lehto V. P., Lehtonen E., Vartio T., Stenman S., Kurki P., Wager O., Small J. V., Dahl D., Badley R. A. Expression of intermediate filaments in cultured cells. J Cell Sci. 1981 Aug;50:45–63. doi: 10.1242/jcs.50.1.45. [DOI] [PubMed] [Google Scholar]
  26. Wick M. R., Scheithauer B. W., Kovacs K. Neuron-specific enolase in neuroendocrine tumors of the thymus, bronchus, and skin. Am J Clin Pathol. 1983 Jun;79(6):703–707. doi: 10.1093/ajcp/79.6.703. [DOI] [PubMed] [Google Scholar]
  27. Yesner R. Spectrum of lung cancer and ectopic hormones. Pathol Annu. 1978;13(Pt 1):207–240. [PubMed] [Google Scholar]
  28. van Muijen G. N., Ruiter D. J., Ponec M., Huiskens-van der, Mey C., Warnaar S. O. Monoclonal antibodies with different specificities against cytokeratins. An immunohistochemical study of normal tissues and tumors. Am J Pathol. 1984 Jan;114(1):9–17. [PMC free article] [PubMed] [Google Scholar]

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