Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1984 May;115(2):245–252.

The immunologic characterization of 95 nodal and extranodal diffuse large cell lymphomas in 89 patients.

R S Doggett, G S Wood, S Horning, R Levy, R F Dorfman, J Bindl, R A Warnke
PMCID: PMC1900485  PMID: 6232854

Abstract

Ninety-five diffuse large cell lymphomas in 89 patients were stained in cryostat sections with a panel of monoclonal antibodies. Lymphoma cells from 47 patients (53%) expressed either kappa or lambda light chains, usually in combination with mu heavy chains. Fifteen samples from 12 patients (14%) expressed two or more T-cell antigens and commonly expressed Ia antigens. Lymphoma cells from 10 of these patients uniformly lacked one or more pan T-cell antigens; lymphoma cells from 4 of these patients also lacked both T-subset antigens--findings which should prove useful in diagnosis. Lymphomas from 28 patients (31%) did not express immunoglobulin or T-cell antigens but commonly expressed the B-lineage antigen B1; and the remaining 9 cases generally expressed Ia antigens, common ALL antigens, or both. Our findings confirm the marked immunologic heterogeneity of diffuse large cell lymphomas; the phenotypic heterogeneity observed in T-cell cases in many instances is difficult to reconcile with current models of T-cell differentiation.

Full text

PDF

Images in this article

248Figure 1
on p.248
249Figure 2
on p.249
249Figure 3
on p.249
250Figure 4
on p.250

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aisenberg A. C., Wilkes B. M., Long J. C., Harris N. L. Cell surface phenotype in lymphoproliferative disease. Am J Med. 1980 Feb;68(2):206–213. doi: 10.1016/0002-9343(80)90355-1. [DOI] [PubMed] [Google Scholar]
  2. Berard C. W., Jaffe E. S., Braylan R. C., Mann R. B., Nanba K. Immunologic aspects and pathology of the malignant lymphomas. Cancer. 1978 Aug;42(2 Suppl):911–921. doi: 10.1002/1097-0142(197808)42:2+<911::aid-cncr2820420713>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  3. Bernard A., Boumsell L., Reinherz E. L., Nadler L. M., Ritz J., Coppin H., Richard Y., Valensi F., Dausset J., Flandrin G. Cell surface characterization of malignant T cells from lymphoblastic lymphoma using monoclonal antibodies: evidence for phenotypic differences between malignant T cells from patients with acute lymphoblastic leukemia and lymphoblastic lymphoma. Blood. 1981 Jun;57(6):1105–1110. [PubMed] [Google Scholar]
  4. Bhan A. K., Nadler L. M., Stashenko P., McCluskey R. T., Schlossman S. F. Stages of B cell differentiation in human lymphoid tissue. J Exp Med. 1981 Sep 1;154(3):737–749. doi: 10.1084/jem.154.3.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bloomfield C. D., Kersey J. H., Brunning R. D., Gajl-Peczalska K. J. Prognostic significance of lymphocyte surface markers in adult non-Hodgkin's malignant lymphoma. Lancet. 1976 Dec 18;2(7999):1330–1333. doi: 10.1016/s0140-6736(76)91976-0. [DOI] [PubMed] [Google Scholar]
  6. Brouet J. C., Preud'Homme J. L., Flandrin G., Chelloul N., Seligmann M. Membrane markers in "histiocytic" lymphomas (reticulum cell sarcomas). J Natl Cancer Inst. 1976 Mar;56(3):631–633. doi: 10.1093/jnci/56.3.631. [DOI] [PubMed] [Google Scholar]
  7. Cossman J., Neckers L. M., Arnold A., Korsmeyer S. J. Induction of differentiation in a case of common acute lymphoblastic leukemia. N Engl J Med. 1982 Nov 11;307(20):1251–1254. doi: 10.1056/NEJM198211113072006. [DOI] [PubMed] [Google Scholar]
  8. DeVita V. T., Jr, Canellos G. P., Chabner B., Schein P., Hubbard S. P., Young R. C. Advanced diffuse histiocytic lymphoma, a potentially curable disease. Lancet. 1975 Feb 1;1(7901):248–250. doi: 10.1016/s0140-6736(75)91142-3. [DOI] [PubMed] [Google Scholar]
  9. Epstein A. L., Levy R., Kim H., Henle W., Henle G., Kaplan H. S. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 1978 Nov;42(5):2379–2391. doi: 10.1002/1097-0142(197811)42:5<2379::aid-cncr2820420539>3.0.co;2-4. [DOI] [PubMed] [Google Scholar]
  10. Filippa D. A., Lieberman P. H., Erlandson R. A., Koziner B., Siegal F. P., Turnbull A., Zimring A., Good R. A. A study of malignant lymphomas using light and ultramicroscopic, cytochemical and immunologic technics: correlation with clinical features. Am J Med. 1978 Feb;64(2):259–268. doi: 10.1016/0002-9343(78)90054-2. [DOI] [PubMed] [Google Scholar]
  11. Greaves M. F., Rao J., Hariri G., Verbi W., Catovsky D., Kung P., Goldstein G. Phenotypic heterogeneity and cellular origins of T cell malignancies. Leuk Res. 1981;5(4-5):281–299. doi: 10.1016/0145-2126(81)90001-1. [DOI] [PubMed] [Google Scholar]
  12. Halper J. P., Knowles D. M., 2nd, Wang C. Y. Ia antigen expression by human malignant lymphomas: correlation with conventional lymphoid markers. Blood. 1980 Mar;55(3):373–382. [PubMed] [Google Scholar]
  13. Korsmeyer S. J., Hieter P. A., Ravetch J. V., Poplack D. G., Waldmann T. A., Leder P. Developmental hierarchy of immunoglobulin gene rearrangements in human leukemic pre-B-cells. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7096–7100. doi: 10.1073/pnas.78.11.7096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Leech J. H., Glick A. D., Waldron J. A., Flexner J. M., Horn R. G., Collins R. D. Malignant lymphomas of follicular center cell origin in man. I. Immunologic studies. J Natl Cancer Inst. 1975 Jan;54(1):11–21. doi: 10.1093/jnci/54.1.11. [DOI] [PubMed] [Google Scholar]
  15. Link M., Warnke R., Finlay J., Amylon M., Miller R., Dilley J., Levy R. A single monoclonal antibody identifies T-cell lineage of childhood lymphoid malignancies. Blood. 1983 Oct;62(4):722–728. [PubMed] [Google Scholar]
  16. Lukes R. J., Collins R. D. Immunologic characterization of human malignant lymphomas. Cancer. 1974 Oct;34(4 Suppl):suppl–suppl:1503. doi: 10.1002/1097-0142(197410)34:8+<1488::aid-cncr2820340822>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  17. Lukes R. J., Taylor C. R., Parker J. W., Lincoln T. L., Pattengale P. K., Tindle B. H. A morphologic and immunologic surface marker study of 299 cases of non-Hodgkin lymphomas and related leukemias. Am J Pathol. 1978 Feb;90(2):461–486. [PMC free article] [PubMed] [Google Scholar]
  18. Pinkus G. S., Said J. W. Characterization of non-Hodgkin's lymphomas using multiple cell markers. Immunologic, morphologic, and cytochemical studies of 72 cases. Am J Pathol. 1979 Feb;94(2):349–380. [PMC free article] [PubMed] [Google Scholar]
  19. Posner M. R., Reinherz E. L., Breard J., Nadler L. M., Rosenthal D. S., Schlossman S. F. Lymphoid subpopulations of peripheral blood and spleen in untreated Hodgkin's disease. Cancer. 1981 Sep 1;48(5):1170–1176. doi: 10.1002/1097-0142(19810901)48:5<1170::aid-cncr2820480522>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
  20. Rudders R. A., Ahl E. T., Jr, DeLellis R. A. Surface marker and histopathologic correlation with long-term survival in advanced large-cell non-Hodgkin's lymphoma. Cancer. 1981 Mar 15;47(6):1329–1335. doi: 10.1002/1097-0142(19810315)47:6<1329::aid-cncr2820470616>3.0.co;2-x. [DOI] [PubMed] [Google Scholar]
  21. Strauchen J. A., Young R. C., DeVita V. T., Jr, Anderson T., Fantone J. C., Berard C. W. Clinical relevance of the histopathological subclassification of diffuse "histiocytic" lymphoma. N Engl J Med. 1978 Dec 21;299(25):1382–1387. doi: 10.1056/NEJM197812212992503. [DOI] [PubMed] [Google Scholar]
  22. Tsan M. F., McIntyre P. A. Stimulation by propylthiouracil of the hexose monophosphate shunt in human polymorphonuclear leucocytes during phagocytosis. Br J Haematol. 1975 Oct;31(2):193–208. doi: 10.1111/j.1365-2141.1975.tb00850.x. [DOI] [PubMed] [Google Scholar]
  23. Warnke R. A., Bindl J., Doggett R. Methods for decreasing interstitial immunoglobulin in tissue slices and cryostat sections. Histochem J. 1983 Jul;15(7):637–644. doi: 10.1007/BF01002985. [DOI] [PubMed] [Google Scholar]
  24. Warnke R., Miller R., Grogan T., Pederson M., Dilley J., Levy R. Immunologic phenotype in 30 patients with diffuse large-cell lymphoma. N Engl J Med. 1980 Aug 7;303(6):293–300. doi: 10.1056/NEJM198008073030601. [DOI] [PubMed] [Google Scholar]
  25. Weintraub J., Warnke R. A. Lymphoma in cardiac allotransplant recipients. Clinical and histological features and immunological phenotype. Transplantation. 1982 Apr;33(4):347–351. doi: 10.1097/00007890-198204000-00002. [DOI] [PubMed] [Google Scholar]
  26. Woda B. A., Racklin B., Rappaport H. Altered expression of histocompatibility antigens on "B" large cell lymphomas. Blood. 1981 Apr;57(4):802–804. [PubMed] [Google Scholar]
  27. Wood G. S., Warnke R. A. The immunologic phenotyping of bone marrow biopsies and aspirates: frozen section techniques. Blood. 1982 May;59(5):913–922. [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES