Abstract
The distribution of lectin binding receptors in human lymphoid tissue (lymph node and tonsil) was studied in an effort to identify lectins with potential application as cell markers. Using a simple peroxidase technique applicable to formalin-fixed, paraffin-embedded tissue, the binding properties of five lectins were investigated: concanavalin A (Con A), Arachis hypogaea (peanut agglutinin), Lotus tetragonolobus (asparagus pea), Glycine max (soybean agglutinin) and Bandeiraea simplicifolia. Three of the lectins were found to demonstrate selective binding in tissue section: Lotus tetragonolobus to transformed follicular center lymphocytes; Arachis hypogaea to transformed follicular center lymphocytes; and Con A to macrophage histiocytes. These lectins are potentially useful markers for the investigation and classification of lymphoproliferative disease.
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- Jacobson W., Stoddart R. W., Collins R. D. Lectin staining of carbohydrates of haemic cells. II. The cells of normal lymphoid origin, of lymphatic leukaemias and related diseases. Histopathology. 1980 Sep;4(5):491–500. doi: 10.1111/j.1365-2559.1980.tb02944.x. [DOI] [PubMed] [Google Scholar]
- Lasser A. The mononuclear phagocytic system: a review. Hum Pathol. 1983 Feb;14(2):108–126. doi: 10.1016/s0046-8177(83)80239-1. [DOI] [PubMed] [Google Scholar]
- Mason D. Y., Taylor C. R. Staining of the A antigen human erythrocytes. J Clin Pathol. 1975 Jul;28(7):594–595. doi: 10.1136/jcp.28.7.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ree H. J., Hsu S. M. Lectin histochemistry of malignant tumors. I. Peanut agglutinin (PNA) receptors in follicular lymphoma and follicular hyperplasia: an immunohistochemical study. Cancer. 1983 May 1;51(9):1631–1638. doi: 10.1002/1097-0142(19830501)51:9<1631::aid-cncr2820510914>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
- Ree H. J. Lectin histochemistry of malignant tumors. II. Concanavalin A: a new histochemical marker for macrophage-histiocytes in follicular lymphoma. Cancer. 1983 May 1;51(9):1639–1646. doi: 10.1002/1097-0142(19830501)51:9<1639::aid-cncr2820510915>3.0.co;2-z. [DOI] [PubMed] [Google Scholar]
- Reisner Y., Biniaminov M., Rosenthal E., Sharon N., Ramot B. Interaction of peanut agglutinin with normal human lymphocytes and with leukemic cells. Proc Natl Acad Sci U S A. 1979 Jan;76(1):447–451. doi: 10.1073/pnas.76.1.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reisner Y., Itzicovitch L., Meshorer A., Sharon N. Hemopoietic stem cell transplantation using mouse bone marrow and spleen cells fractionated by lectins. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2933–2936. doi: 10.1073/pnas.75.6.2933. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rose M. L., Birbeck M. S., Wallis V. J., Forrester J. A., Davies A. J. Peanut lectin binding properties of germinal centres of mouse lymphoid tissue. Nature. 1980 Mar 27;284(5754):364–366. doi: 10.1038/284364a0. [DOI] [PubMed] [Google Scholar]
- Sharon N., Lis H. Lectins: cell-agglutinating and sugar-specific proteins. Science. 1972 Sep 15;177(4053):949–959. doi: 10.1126/science.177.4053.949. [DOI] [PubMed] [Google Scholar]
- Stoddart R. W., Collins R. D., Jacobson W. Lectin staining of carbohydrates of haemic cells; the cells of normal blood and bone marrow and of the myeloid leukaemias. J Pathol. 1980 Aug;131(4):321–332. doi: 10.1002/path.1711310404. [DOI] [PubMed] [Google Scholar]
- Strauchen J. A. Fluoresceinated lectins as probe for cell surface changes associated with lymphocyte transformation. Am J Hematol. 1982 May;12(3):227–232. doi: 10.1002/ajh.2830120304. [DOI] [PubMed] [Google Scholar]
- Watanabe S., Nakajima T., Shimosato Y., Sato Y., Shimizu K. Malignant histiocytosis and Letterer-Siwe disease. Neoplasms of T-zone histiocyte with S100 protein. Cancer. 1983 Apr 15;51(8):1412–1424. doi: 10.1002/1097-0142(19830415)51:8<1412::aid-cncr2820510815>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]