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. 1984 Aug;116(2):245–252.

Distribution and kinetics of mononuclear phagocytes in granulomas elicited by eggs of Schistosoma mansoni.

M J Stadecker, J A Wright
PMCID: PMC1900545  PMID: 6205592

Abstract

Previous work has shown that cell suspensions from egg granulomas of Schistosoma mansoni-infected mice contain populations of both I-A-positive and -negative granuloma macrophages (GMs). The present study was undertaken to investigate the distribution of I-A-bearing macrophages within the granulomas, as well as the kinetics of I-A antigen expression by these cells in vivo. Cryostat sections of liver tissue from infected animals, stained with monoclonal anti-I-A antibodies, demonstrated the presence of I-A-positive GMs in peripheral areas of the granulomas, whereas I-A-negative cells concentrated in their centers. For investigation of their expression of I-A antigen, dispersed GMs were studied at time intervals after subjecting infected mice to lethal doses of total body irradiation. I-A half-life on GMs in vivo was estimated to be 2 days, based both on visual detection by immunofluorescence and functionally on the ability of GMs to perform as antigen-presenting cells. Autoradiographic studies, performed on infected liver tissue obtained 1 hour after in vivo administration of tritiated thymidine, showed that macrophages predominantly replicated in peripheral areas of the schistosomal egg granulomas. After longer intervals, however, labeled cells were seen in more central areas of the granuloma, suggesting an overall cell flux from the periphery to the center. These findings indicate that macrophages express I-A antigens in peripheral areas of the granulomas, where macrophage replication and recruitment from the bone marrow take place. They suggest that I-A expression occurs during a limited period of time in "young" macrophages, which later may convert to an I-A-negative phenotype.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams D. O. The granulomatous inflammatory response. A review. Am J Pathol. 1976 Jul;84(1):164–192. [PMC free article] [PubMed] [Google Scholar]
  2. Beller D. I., Unanue E. R. Regulation of macrophage populations. II. Synthesis and expression of Ia antigens by peritoneal exudate macrophages is a transient event. J Immunol. 1981 Jan;126(1):263–269. [PubMed] [Google Scholar]
  3. Boros D. L. Granulomatous inflammations. Prog Allergy. 1978;24:183–267. doi: 10.1159/000401230. [DOI] [PubMed] [Google Scholar]
  4. Chensue S. W., Boros D. L. Modulation of granulomatous hypersensitivity. I. Characterization of T lymphocytes involved in the adoptive suppression of granuloma formation in Schistosoma mansoni-infected mice. J Immunol. 1979 Sep;123(3):1409–1414. [PubMed] [Google Scholar]
  5. Chensue S. W., Boros D. L. Population dynamics of T and B lymphocytes in the lymphoid organs, circulation, and granulomas of mice infected with Schistosoma mansoni. Am J Trop Med Hyg. 1979 Mar;28(2):291–299. doi: 10.4269/ajtmh.1979.28.291. [DOI] [PubMed] [Google Scholar]
  6. Chensue S. W., Kunkel S. L., Higashi G. I., Ward P. A., Boros D. L. Production of superoxide anion, prostaglandins, and hydroxyeicosatetraenoic acids by macrophages from hypersensitivity-type (Schistosoma mansoni egg) and foreign body-type granulomas. Infect Immun. 1983 Dec;42(3):1116–1125. doi: 10.1128/iai.42.3.1116-1125.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chensue S. W., Kunkel S. L., Ward P. A., Higashi G. I. Exogenously administered prostaglandins modulate pulmonary granulomas induced by Schistosoma mansoni eggs. Am J Pathol. 1983 Apr;111(1):78–87. [PMC free article] [PubMed] [Google Scholar]
  8. Chensue S. W., Wellhausen S. R., Boros D. L. Modulation of granulomatous hypersensitivity. II. Participation of Ly 1+ and Ly 2+ T lymphocytes in the suppression of granuloma formation and lymphokine production in Schistosoma mansoni-infected mice. J Immunol. 1981 Jul;127(1):363–367. [PubMed] [Google Scholar]
  9. Colley D. G. T lymphocytes that contribute to the immunoregulation of granuloma formation in chronic murine schistosomiasis. J Immunol. 1981 Apr;126(4):1465–1468. [PubMed] [Google Scholar]
  10. Courtade E. T., Tsuda T., Thomas C. R., Dannenberg A. M. Capillary density in developing and healing tuberculous lesions produced by BCG in rabbits. A quantitative study. Am J Pathol. 1975 Feb;78(2):243–260. [PMC free article] [PubMed] [Google Scholar]
  11. Green W. F., Colley D. G. Modulation of Schistosoma mansoni egg-induced granuloma formation: I-J restriction of T cell-mediated suppression in a chronic parasitic infection. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1152–1156. doi: 10.1073/pnas.78.2.1152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scher M. G., Beller D. I., Unanue E. R. Demonstration of a soluble mediator that induces exudates rich in Ia-positive macrophages. J Exp Med. 1980 Dec 1;152(6):1684–1698. doi: 10.1084/jem.152.6.1684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scher M. G., Unanue E. R., Beller D. I. Regulation of macrophage populations. III. The immunologic induction of exudates rich in Ia-bearing macrophages is a radiosensitive process. J Immunol. 1982 Jan;128(1):447–450. [PubMed] [Google Scholar]
  14. Spector W. G., Willoughby D. A. The origin of mononuclear cells in chronic inflammation and tuberculin reactions in the rat. J Pathol Bacteriol. 1968 Oct;96(2):389–399. doi: 10.1002/path.1700960217. [DOI] [PubMed] [Google Scholar]
  15. Stadecker M. J., Wyler D. J., Wright J. A. Ia antigen expression and antigen-presenting function by macrophages isolated from hypersensitivity granulomas. J Immunol. 1982 Jun;128(6):2739–2744. [PubMed] [Google Scholar]
  16. Steeg P. S., Moore R. N., Johnson H. M., Oppenheim J. J. Regulation of murine macrophage Ia antigen expression by a lymphokine with immune interferon activity. J Exp Med. 1982 Dec 1;156(6):1780–1793. doi: 10.1084/jem.156.6.1780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stenger R. J., Warren K. S., Johnson E. A. An electron microscopic study of the liver parenchyma and schistosome pigment in murine hepatosplenic schistosomiasis mansoni. Am J Trop Med Hyg. 1967 Jul;16(4):473–482. doi: 10.4269/ajtmh.1967.16.473. [DOI] [PubMed] [Google Scholar]
  18. Sunday M. E., Stadecker M. J., Wright J. A., Aoki I., Dorf M. E. Induction of immune responses by schistosome granuloma macrophages. J Immunol. 1983 May;130(5):2413–2417. [PubMed] [Google Scholar]
  19. Tsuda T., Dannenberg A. M., Ando M., Abbey H., Corrin A. R. Mononuclear cell turnover in chronic inflammation: studies on tritiated thymidine-labeled cells in blood, tuberculin traps, and dermal BCG lesions of rabbits. Am J Pathol. 1976 May;83(2):255–268. [PMC free article] [PubMed] [Google Scholar]
  20. Unanue E. R. The regulatory role of macrophages in antigenic stimulation. Part Two: symbiotic relationship between lymphocytes and macrophages. Adv Immunol. 1981;31:1–136. doi: 10.1016/s0065-2776(08)60919-0. [DOI] [PubMed] [Google Scholar]
  21. Weinstock J. V., Boros D. L. Organ-dependent differences in composition and function observed in hepatic and intestinal granulomas isolated from mice with Schistosomiasis mansoni. J Immunol. 1983 Jan;130(1):418–422. [PubMed] [Google Scholar]

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