Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1996 Jun;70(6):3688–3697. doi: 10.1128/jvi.70.6.3688-3697.1996

Epitopes and hemagglutination binding domain on subgenus B:2 adenovirus fibers.

Y F Mei 1, G Wadell 1
PMCID: PMC190244  PMID: 8648703

Abstract

The adenovirus fiber serves as a ligand between the virus and the host cell receptor and manifests hemagglutination (HA) activity and antigenic domains. We have screened both the antigenic and immunogenic epitopes on the adenovirus fibers of subgenus B:2 by using recombinant fiber proteins (rfibers) expressed in Escherichia coli, synthesized peptides (P1 to P8), and the corresponding antisera. The results indicated that P4 (amino acids [aa] 201 to 220), P5 (aa 231 to 250), and P7 (aa 275 to 295) presented both antigenic and immunogenic epitopes in adenovirus type 11 prototype (Ad11p), Ad34a, and Ad11a fibers. P6 (aa 251 to 270) presented both epitopes in Ad11a fiber but only an antigenic epitope in other fibers. The C-terminal 20 amino acids of the fiber, corresponding to P8, manifested an epitope of low-level immunogenicity. P5, localized at the N-terminal aa 231 to 250, displayed an epitope that reacted with fibers of all the members of subgenus B analyzed. The rfibers of Ad11p and Ad34a displayed HA activity with monkey erythrocytes, though those of Ad11a did not. Mutagenesis of the rfibers revealed that neither the fragment replacements, 11p20211a, llp26011a,and 11a28011p, nor the Ad11p rfiber with the substitutions of Tyr-260-->H (Tyr260H)and Arg279Q displayed HA activity. The Ad11a fiber knob was sensitive to proteolytic digestion, whereas that of Ad11p was resistant. The results demonstrated that the decisive HA binding domain was presented at aa 260 to 280 and was conformation dependent. Nearby amino acids, aa 283 and 284, may also affect the HA function.

Full Text

The Full Text of this article is available as a PDF (817.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albiges-Rizo C., Barge A., Ruigrok R. W., Timmins P. A., Chroboczek J. Human adenovirus serotype 3 fiber protein. Comparison of native and recombinant proteins. J Biol Chem. 1991 Feb 25;266(6):3961–3967. [PubMed] [Google Scholar]
  2. Allard A., Girones R., Juto P., Wadell G. Polymerase chain reaction for detection of adenoviruses in stool samples. J Clin Microbiol. 1990 Dec;28(12):2659–2667. doi: 10.1128/jcm.28.12.2659-2667.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bai M., Harfe B., Freimuth P. Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell-rounding activity and delay virus reproduction in flat cells. J Virol. 1993 Sep;67(9):5198–5205. doi: 10.1128/jvi.67.9.5198-5205.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boudin M. L., Rigolet M., Lemay P., Galibert F., Boulanger P. Biochemical and genetical characterization of a fiber-defective temperature-sensitive mutant of type 2 adenovirus. EMBO J. 1983;2(11):1921–1927. doi: 10.1002/j.1460-2075.1983.tb01680.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Devaux C., Caillet-Boudin M. L., Jacrot B., Boulanger P. Crystallization, enzymatic cleavage, and the polarity of the adenovirus type 2 fiber. Virology. 1987 Nov;161(1):121–128. doi: 10.1016/0042-6822(87)90177-2. [DOI] [PubMed] [Google Scholar]
  6. Ellis J. Proteins as molecular chaperones. 1987 Jul 30-Aug 5Nature. 328(6129):378–379. doi: 10.1038/328378a0. [DOI] [PubMed] [Google Scholar]
  7. Emini E. A., Hughes J. V., Perlow D. S., Boger J. Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide. J Virol. 1985 Sep;55(3):836–839. doi: 10.1128/jvi.55.3.836-839.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Flomenberg P. R., Chen M., Munk G., Horwitz M. S. Molecular epidemiology of adenovirus type 35 infections in immunocompromised hosts. J Infect Dis. 1987 Jun;155(6):1127–1134. doi: 10.1093/infdis/155.6.1127. [DOI] [PubMed] [Google Scholar]
  9. Flomenberg P., Babbitt J., Drobyski W. R., Ash R. C., Carrigan D. R., Sedmak G. V., McAuliffe T., Camitta B., Horowitz M. M., Bunin N. Increasing incidence of adenovirus disease in bone marrow transplant recipients. J Infect Dis. 1994 Apr;169(4):775–781. doi: 10.1093/infdis/169.4.775. [DOI] [PubMed] [Google Scholar]
  10. Goloubinoff P., Gatenby A. A., Lorimer G. H. GroE heat-shock proteins promote assembly of foreign prokaryotic ribulose bisphosphate carboxylase oligomers in Escherichia coli. Nature. 1989 Jan 5;337(6202):44–47. doi: 10.1038/337044a0. [DOI] [PubMed] [Google Scholar]
  11. Green N. M., Wrigley N. G., Russell W. C., Martin S. R., McLachlan A. D. Evidence for a repeating cross-beta sheet structure in the adenovirus fibre. EMBO J. 1983;2(8):1357–1365. doi: 10.1002/j.1460-2075.1983.tb01592.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Henry L. J., Xia D., Wilke M. E., Deisenhofer J., Gerard R. D. Characterization of the knob domain of the adenovirus type 5 fiber protein expressed in Escherichia coli. J Virol. 1994 Aug;68(8):5239–5246. doi: 10.1128/jvi.68.8.5239-5246.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hierholzer J. C. Adenoviruses in the immunocompromised host. Clin Microbiol Rev. 1992 Jul;5(3):262–274. doi: 10.1128/cmr.5.3.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hierholzer J. C., Pumarola A. Antigenic characterization of intermediate adenovirus 14-11 strains associated with upper respiratory illness in a military camp. Infect Immun. 1976 Feb;13(2):354–359. doi: 10.1128/iai.13.2.354-359.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hierholzer J. C., Pumarola A., Rodriguez-Torres A., Beltran M. Occurrence of respiratory illness due to an atypical strain of adenovirus type 11 during a large outbreak in Spanish military recruits. Am J Epidemiol. 1974 Jun;99(6):434–442. doi: 10.1093/oxfordjournals.aje.a121632. [DOI] [PubMed] [Google Scholar]
  16. Houghten R. A. General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5131–5135. doi: 10.1073/pnas.82.15.5131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Louis N., Fender P., Barge A., Kitts P., Chroboczek J. Cell-binding domain of adenovirus serotype 2 fiber. J Virol. 1994 Jun;68(6):4104–4106. doi: 10.1128/jvi.68.6.4104-4106.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mei Y. F., Wadell G. Hemagglutination properties and nucleotide sequence analysis of the fiber gene of adenovirus genome types 11p and 11a. Virology. 1993 Jun;194(2):453–462. doi: 10.1006/viro.1993.1284. [DOI] [PubMed] [Google Scholar]
  19. Mei Y. F., Wadell G. Highly heterogeneous fiber genes in the two closely related adenovirus genome types Ad35p and Ad34a. Virology. 1995 Jan 10;206(1):686–689. doi: 10.1016/s0042-6822(95)80089-1. [DOI] [PubMed] [Google Scholar]
  20. Murtagh P., Cerqueiro C., Halac A., Avila M., Kajon A. Adenovirus type 7h respiratory infections: a report of 29 cases of acute lower respiratory disease. Acta Paediatr. 1993 Jun-Jul;82(6-7):557–561. doi: 10.1111/j.1651-2227.1993.tb12753.x. [DOI] [PubMed] [Google Scholar]
  21. Myerowitz R. L., Stalder H., Oxman M. N., Levin M. J., Moore M., Leith J. D., Gantz N. M., Hierholzer J. C., Hierholzer J. C. Fatal disseminated adenovirus infection in a renal transplant recipient. Am J Med. 1975 Oct;59(4):591–598. doi: 10.1016/0002-9343(75)90267-3. [DOI] [PubMed] [Google Scholar]
  22. Norrby E. The relationship between the soluble antigens and the virion of adenovirus type 3. IV. Immunological complexity of soluble components. Virology. 1969 Apr;37(4):565–576. doi: 10.1016/0042-6822(69)90274-8. [DOI] [PubMed] [Google Scholar]
  23. Norrby E., Wadell G., Marusyk H. Fiber-associated incomplete and complete hemagglutinins of adenovirus type 6. Arch Gesamte Virusforsch. 1969;28(2):239–244. doi: 10.1007/BF01249389. [DOI] [PubMed] [Google Scholar]
  24. Novelli A., Boulanger P. A. Assembly of adenovirus type 2 fiber synthesized in cell-free translation system. J Biol Chem. 1991 May 15;266(14):9299–9303. [PubMed] [Google Scholar]
  25. Novelli A., Boulanger P. A. Deletion analysis of functional domains in baculovirus-expressed adenovirus type 2 fiber. Virology. 1991 Nov;185(1):365–376. doi: 10.1016/0042-6822(91)90784-9. [DOI] [PubMed] [Google Scholar]
  26. Numazaki Y., Shigeta S., Kumasaka T., Miyazawa T., Yamanaka M., Yano N., Takai S., Ishida N. Acute hemorrhagic cystitis in children. Isolation of adenovirus type II. N Engl J Med. 1968 Mar 28;278(13):700–704. doi: 10.1056/NEJM196803282781303. [DOI] [PubMed] [Google Scholar]
  27. Rothman J. E. Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells. Cell. 1989 Nov 17;59(4):591–601. doi: 10.1016/0092-8674(89)90005-6. [DOI] [PubMed] [Google Scholar]
  28. Shields A. F., Hackman R. C., Fife K. H., Corey L., Meyers J. D. Adenovirus infections in patients undergoing bone-marrow transplantation. N Engl J Med. 1985 Feb 28;312(9):529–533. doi: 10.1056/NEJM198502283120901. [DOI] [PubMed] [Google Scholar]
  29. Shinagawa M., Matsuda A., Ishiyama T., Goto H., Sato G. A rapid and simple method for preparation of adenovirus DNA from infected cells. Microbiol Immunol. 1983;27(9):817–822. doi: 10.1111/j.1348-0421.1983.tb00638.x. [DOI] [PubMed] [Google Scholar]
  30. Shindo K., Kitayama T., Ura T., Matsuya F., Kusaba Y., Kanetake H., Saito Y. Acute hemorrhagic cystitis caused by adenovirus type 11 after renal transplantation. Urol Int. 1986;41(2):152–155. doi: 10.1159/000281186. [DOI] [PubMed] [Google Scholar]
  31. Stevenson S. C., Rollence M., White B., Weaver L., McClelland A. Human adenovirus serotypes 3 and 5 bind to two different cellular receptors via the fiber head domain. J Virol. 1995 May;69(5):2850–2857. doi: 10.1128/jvi.69.5.2850-2857.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 1990;185:60–89. doi: 10.1016/0076-6879(90)85008-c. [DOI] [PubMed] [Google Scholar]
  33. Wadell G., Hammarskjöld M. L., Winberg G., Varsanyi T. M., Sundell G. Genetic variability of adenoviruses. Ann N Y Acad Sci. 1980;354:16–42. doi: 10.1111/j.1749-6632.1980.tb27955.x. [DOI] [PubMed] [Google Scholar]
  34. Wadell G. Hemagglutination with adenovirus serotypes belonging to Rosen's subgroups II and 3. Proc Soc Exp Biol Med. 1969 Nov;132(2):413–421. doi: 10.3181/00379727-132-34227. [DOI] [PubMed] [Google Scholar]
  35. Watson G., Burdon M. G., Russell W. C. An antigenic analysis of the adenovirus type 2 fibre polypeptide. J Gen Virol. 1988 Mar;69(Pt 3):525–535. doi: 10.1099/0022-1317-69-3-525. [DOI] [PubMed] [Google Scholar]
  36. Wickham T. J., Mathias P., Cheresh D. A., Nemerow G. R. Integrins alpha v beta 3 and alpha v beta 5 promote adenovirus internalization but not virus attachment. Cell. 1993 Apr 23;73(2):309–319. doi: 10.1016/0092-8674(93)90231-e. [DOI] [PubMed] [Google Scholar]
  37. Xia D., Henry L. J., Gerard R. D., Deisenhofer J. Crystal structure of the receptor-binding domain of adenovirus type 5 fiber protein at 1.7 A resolution. Structure. 1994 Dec 15;2(12):1259–1270. doi: 10.1016/s0969-2126(94)00126-x. [DOI] [PubMed] [Google Scholar]
  38. de Jong P. J., Valderrama G., Spigland I., Horwitz M. S. Adenovirus isolates from urine of patients with acquired immunodeficiency syndrome. Lancet. 1983 Jun 11;1(8337):1293–1296. doi: 10.1016/s0140-6736(83)92411-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES