Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1980 Sep;100(3):707–725.

Effects of epidermal growth factor on lung maturation in fetal lambs.

H W Sundell, M E Gray, F S Serenius, M B Escobedo, M T Stahlman
PMCID: PMC1903564  PMID: 6968161

Abstract

The ability of epidermal growth factor (EGF) to induce lung maturation was evaluated in fetal and neonatal lambs. EGF was infused (3-5 days) into one member of 10 fetal twin pairs, one member of 2 term twin pairs, and 2 singleton term lambs. All EGF-treated lambs had evidence of epithelial hyperplasia of the conducting airways typical of the EGF effect. With the exception of the most immature pair, the lungs of treated versus control lambs were judged more mature by morphologic criteria by use of light and electron microscopy. None of the 6 premature lambs treated with EGF and allowed to breath showed evidence of hyaline membrane disease, while 3 untreated control lambs developed typical hyaline membranes when delivered by cesarean section after maternal hypotension. All untreated control animals showed more severe clinical symptoms of respiratory distress than did the EGF-treated animals.

Full text

PDF
707

Images in this article

724Figure 8
on p.724
724Figure 9
on p.724
720Figure 1
on p.720
720Figure 2
on p.720
721Figure 3
on p.721
721Figure 4
on p.721
725Figure 10
on p.725
725Figure 11
on p.725
722Figure 5
on p.722
722Figure 6
on p.722
723Figure 7
on p.723

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armelin H. A. Pituitary extracts and steroid hormones in the control of 3T3 cell growth. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2702–2706. doi: 10.1073/pnas.70.9.2702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ballard R. A., Ballard P. L. Use of prenatal glucocorticoid therapy to prevent respiratory distress syndrome. A supporting view. Am J Dis Child. 1976 Sep;130(9):982–987. doi: 10.1001/archpedi.1976.02120100072011. [DOI] [PubMed] [Google Scholar]
  3. Barrett C. T., Sevanian A., Phelps D. L., Gilden C., Kaplan S. A. Effects of cortisol and aminophylline upon survival, pulmonary mechanics, and secreted phosphatidyl choline of prematurely delivered rabbits. Pediatr Res. 1978 Jan;12(1):38–42. doi: 10.1203/00006450-197801000-00010. [DOI] [PubMed] [Google Scholar]
  4. COHEN S. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem. 1962 May;237:1555–1562. [PubMed] [Google Scholar]
  5. Carpenter G., Cohen S. Epidermal growth factor. Annu Rev Biochem. 1979;48:193–216. doi: 10.1146/annurev.bi.48.070179.001205. [DOI] [PubMed] [Google Scholar]
  6. Catterton W. Z., Escobedo M. B., Sexson W. R., Gray M. E., Sundell H. W., Stahlman M. T. Effect of epidermal growth factor on lung maturation in fetal rabbits. Pediatr Res. 1979 Feb;13(2):104–108. doi: 10.1203/00006450-197902000-00004. [DOI] [PubMed] [Google Scholar]
  7. Gregory H. Isolation and structure of urogastrone and its relationship to epidermal growth factor. Nature. 1975 Sep 25;257(5524):325–327. doi: 10.1038/257325a0. [DOI] [PubMed] [Google Scholar]
  8. Hamosh M., Hamosh P. The effect of prolactin on the lecithin content of fetal rabbit lung. J Clin Invest. 1977 May;59(5):1002–1005. doi: 10.1172/JCI108693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hollenberg M. D., Cuatrecasas P. Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2964–2968. doi: 10.1073/pnas.70.10.2964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones R. O. The in vitro effect of epithelial growth factor on rat organ cultures. Exp Cell Res. 1966 Oct;43(3):645–656. doi: 10.1016/0014-4827(66)90036-x. [DOI] [PubMed] [Google Scholar]
  11. Kotas R. V., Avery M. E. Accelerated appearance of pulmonary surfactant in the fetal rabbit. J Appl Physiol. 1971 Mar;30(3):358–361. doi: 10.1152/jappl.1971.30.3.358. [DOI] [PubMed] [Google Scholar]
  12. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Liggins G. C. Premature delivery of foetal lambs infused with glucocorticoids. J Endocrinol. 1969 Dec;45(4):515–523. doi: 10.1677/joe.0.0450515. [DOI] [PubMed] [Google Scholar]
  14. Liggins G. C. Premature parturition after infusion of corticotrophin or cortisol into foetal lambs. J Endocrinol. 1968 Oct;42(2):323–329. doi: 10.1677/joe.0.0420323. [DOI] [PubMed] [Google Scholar]
  15. Platzker A. C., Kitterman J. A., Mescher E. J., Clements J. A., Tooley W. H. Surfactant in the lung and tracheal fluid of the fetal lamb and acceleration of its appearance by dexamethasone. Pediatrics. 1975 Oct;56(4):554–561. [PubMed] [Google Scholar]
  16. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rheinwald J. G., Green H. Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature. 1977 Feb 3;265(5593):421–424. doi: 10.1038/265421a0. [DOI] [PubMed] [Google Scholar]
  18. STAHLMAN M., LEQUIRE V. S., YOUNG W. C., MERRILL R. E., BIRMINGHAM R. T., PAYNE G. A., GRAY J. PATHOPHYSIOLOGY OF RESPIRATORY DISTRESS IN NEWBORN LAMBS. CIRCULATORY, BIOCHEMICAL, AND PATHOLOGICAL CONSIDERATIONS. Am J Dis Child. 1964 Oct;108:375–393. doi: 10.1001/archpedi.1964.02090010377008. [DOI] [PubMed] [Google Scholar]
  19. Savage C. R., Jr, Inagami T., Cohen S. The primary structure of epidermal growth factor. J Biol Chem. 1972 Dec 10;247(23):7612–7621. [PubMed] [Google Scholar]
  20. Smith B. T., Giroud C. J., Robert M., Avery M. E. Insulin antagonism of cortisol action on lechithin synthesis by cultured fetal lung cells. J Pediatr. 1975 Dec;87(6 Pt 1):953–955. doi: 10.1016/s0022-3476(75)80916-4. [DOI] [PubMed] [Google Scholar]
  21. Starkey R. H., Cohen S., Orth D. N. Epidermal growth factor: identification of a new hormone in human urine. Science. 1975 Sep 5;189(4205):800–802. doi: 10.1126/science.1172293. [DOI] [PubMed] [Google Scholar]
  22. Strott C. A., Sundel H., Stahlman M. T. Maternal and fetal plasma progesterone, cortisol, testosterone and 17beta-estradiol in preparturient sheep: response to fetal ACTH infusion. Endocrinology. 1974 Nov;95(5):1327–1339. doi: 10.1210/endo-95-5-1327. [DOI] [PubMed] [Google Scholar]
  23. Sundell H. W., Gray M. E., Relier J. P., Kovar I. Z., Catterton W. Z., Swift L. L., Stahlman M. T. The effects of ACTH on lung maturation in fetal lambs. Am J Pathol. 1979 Nov;97(2):393–410. [PMC free article] [PubMed] [Google Scholar]
  24. Taeusch H. W., Jr, Carson S. H., Wang N. S., Avery M. E. Heroin induction of lung maturation and growth retardation in fetal rabbits. J Pediatr. 1973 May;82(5):869–875. doi: 10.1016/s0022-3476(73)80086-1. [DOI] [PubMed] [Google Scholar]
  25. Wang N. S., Kotas R. V., Avery M. E., Thurlbeck W. M. Accelerated appearance of osmiophilic bodies in fetal lungs following steroid injection. J Appl Physiol. 1971 Mar;30(3):362–365. doi: 10.1152/jappl.1971.30.3.362. [DOI] [PubMed] [Google Scholar]
  26. Wu B., Kikkawa Y., Orzalesi M. M., Motoyama E. K., Kaibara M., Zigas C. J., Cook C. D. The effect of thyroxine on the maturation of fetal rabbit lungs. Biol Neonate. 1973;22(3):161–168. doi: 10.1159/000240550. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES