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. 1980 Oct;101(1):17–30.

Monocytic orchitis and aspermatogenesis in normal and vasectomized rhesus macaques (Macaca mulatta).

K S Tung, N J Alexander
PMCID: PMC1903595  PMID: 7446699

Abstract

The immunopathologic findings in the testes and kidneys of 25 long-term vasectomized monkeys and of 13 age-matched control animals were compared. Antisperm antibody was found in serum samples from 60% of vasectomized monkeys and in no samples from control animals. Orchitis, aspermatogenesis, or both, resembling allergic orchitis, was noted in 92% of vasectomized animals and in 23% of controls. Epididymitis and epididymal granuloma occurred exclusively in the vasectomized animals (52%). By immunofluorescence, granular deposits of IgA, IgG, and/or C3 in the basal lamina of the ductuli efferentes and the caput epididymidis were detected in 45% of the control monkeys. In vasectomized animals, the incidence of immune deposits increased to 91%, and the deposits became more widespread. Although both vasectomized and control animals had IgM, IgG, or both, in the glomerular mesangial region, C3 was found in vasectomized monkeys only (29%). The results of this study indicate that monocytic infiltrations with aspermatogenesis in association with immune deposits along the excurrent duct normally occur in subhuman primates, and that these changes increase in incidence after vasectomy. Although the testicular disease highly resembles allergic orchitis, an immunologic basis remains to be established.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander N. J. Autoimmune hypospermatogenesis in vasectomized guinea pigs. Contraception. 1973 Aug;8(2):147–164. doi: 10.1016/0010-7824(73)90121-2. [DOI] [PubMed] [Google Scholar]
  2. Alexander N. J., Tung K. S. Immunological and morphological effects of vasectomy in the rabbit. Anat Rec. 1977 Jul;188(3):339–350. doi: 10.1002/ar.1091880307. [DOI] [PubMed] [Google Scholar]
  3. Alexander N. J. Vasectomy: long-term effects in the rhesus monkey. J Reprod Fertil. 1972 Dec;31(3):399–406. doi: 10.1530/jrf.0.0310399. [DOI] [PubMed] [Google Scholar]
  4. Alexander N. J., Wilson B. J., Patterson G. D. Vasectomy: immunologic effects in rhesus monkeys and men. Fertil Steril. 1974 Feb;25(2):149–156. [PubMed] [Google Scholar]
  5. Andrada J. A., Andrada E. C., Witebsky E. Experimental autoallergic orchitis in rhesus monkeys. Proc Soc Exp Biol Med. 1969 Apr;130(4):1106–1113. doi: 10.3181/00379727-130-33729. [DOI] [PubMed] [Google Scholar]
  6. Ansbacher R., Keung-Yeung K., Wurster J. C. Sperm antibodies in vasectomized men. Fertil Steril. 1972 Sep;23(9):640–643. [PubMed] [Google Scholar]
  7. Bedford J. M. Adaptations of the male reproductive tract and the fate of spermatozoa following vasectomy in the rabbit, rhesus monkey, hamster and rat. Biol Reprod. 1976 Mar;14(2):118–142. doi: 10.1095/biolreprod14.2.118. [DOI] [PubMed] [Google Scholar]
  8. Bigazzi P. E., Kosuda L. L., Hsu K. C., Andres G. A. Immune complex orchitis in vasectomized rabbits. J Exp Med. 1976 Feb 1;143(2):382–404. doi: 10.1084/jem.143.2.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Braley J. F., Dawson C. A., Moore V. L. Immunologic block against antigen absorption from isolated perfused rabbit lungs. J Immunol. 1978 Sep;121(3):926–929. [PubMed] [Google Scholar]
  10. Dym M., Fawcett D. W. The blood-testis barrier in the rat and the physiological compartmentation of the seminiferous epithelium. Biol Reprod. 1970 Dec;3(3):308–326. doi: 10.1093/biolreprod/3.3.308. [DOI] [PubMed] [Google Scholar]
  11. Dym M. The fine structure of the monkey (Macaca) Sertoli cell and its role in maintaining the blood-testis barrier. Anat Rec. 1973 Apr;175(4):639–656. doi: 10.1002/ar.1091750402. [DOI] [PubMed] [Google Scholar]
  12. Dym M. The mammalian rete testis--a morphological examination. Anat Rec. 1976 Dec;186(4):493–523. doi: 10.1002/ar.1091860404. [DOI] [PubMed] [Google Scholar]
  13. Fritz T. E., Lombard S. A., Tyler S. A., Norris W. P. Pathology and familial incidence of orchitis and its relation to thyroiditis in a closed beagle colony. Exp Mol Pathol. 1976 Apr;24(2):142–158. doi: 10.1016/0014-4800(76)90002-2. [DOI] [PubMed] [Google Scholar]
  14. Galle J., Friend D. S. The fate of sperm after vasectomy in the guinea pig: fine structure, freeze-fracture, and cytochemistry. Lab Invest. 1977 Jul;37(1):79–95. [PubMed] [Google Scholar]
  15. Johnson A. L., Howards S. S. Intratubular hydrostatic pressure in testis and epididymis before and after vasectomy. Am J Physiol. 1975 Feb;228(2):556–564. doi: 10.1152/ajplegacy.1975.228.2.556. [DOI] [PubMed] [Google Scholar]
  16. KIBRICK S., BELDING D. L., MERRILL B. Methods for the detection of antibodies against mammalian spermatozoa. II. A gelatin agglutination test. Fertil Steril. 1952 Sep-Oct;3(5):430–438. doi: 10.1016/s0015-0282(16)31026-3. [DOI] [PubMed] [Google Scholar]
  17. Kolk A. H., Samuel T., Rümke P. Auto-antigens of human spermatozoa. I. Solubilization of a new auto-antigen detected on swollen spermheads. Clin Exp Immunol. 1974 Jan;16(1):63–76. [PMC free article] [PubMed] [Google Scholar]
  18. PHADKE A. M., PADUKONE K. PRESENCE AND SIGNIFICANCE OF AUTOANTIBODIES AGAINST SPERMATOZOA IN THE BLOOD OF MEN WITH OBSTRUCTED VAS DEFERENS. J Reprod Fertil. 1964 Apr;7:162–170. [PubMed] [Google Scholar]
  19. Shulman S., Zappi E., Ahmed U., Davis J. E. Immunologic consequences of vasectomy. Contraception. 1972 Apr;5(4):269–278. doi: 10.1016/0010-7824(72)90066-2. [DOI] [PubMed] [Google Scholar]
  20. Suzuki F., Nagano T. Regional differentiation of cell junctions in the excurrent duct epithelium of the rat testis as revealed by freeze-fracture. Anat Rec. 1978 Aug;191(4):503–519. doi: 10.1002/ar.1091910409. [DOI] [PubMed] [Google Scholar]
  21. Tung K. S. A useful technique applicable to the immunofluorescence test. J Immunol Methods. 1977;18(3-4):391–392. doi: 10.1016/0022-1759(77)90194-6. [DOI] [PubMed] [Google Scholar]
  22. Tung K. S., Alexander N. J. Immunopathologic studies on vasectomized guinea pigs. Biol Reprod. 1977 Sep;17(2):241–254. doi: 10.1095/biolreprod17.2.241. [DOI] [PubMed] [Google Scholar]
  23. Tung K. S. Allergic orchitis lesions are adoptively transferred from vasoligated guinea pigs to syngeneic recipients. Science. 1978 Sep 1;201(4358):833–835. doi: 10.1126/science.684410. [DOI] [PubMed] [Google Scholar]
  24. Tung K. S. Human sperm antigens and antisperm antibodies I. Studies on vasectomy patients. Clin Exp Immunol. 1975 Apr;20(1):93–104. [PMC free article] [PubMed] [Google Scholar]
  25. Tung K. S., Unanue E. R., Dixon F. J. Pathogenesis of experimental allergic orchitis. II. The role of antibody. J Immunol. 1971 Jun;106(6):1463–1472. [PubMed] [Google Scholar]
  26. Tung K. S., Woodroffe A. J. Immunopathology of experimental allergic orchitis in the rabbit. J Immunol. 1978 Jan;120(1):320–328. [PubMed] [Google Scholar]
  27. Walker W. A., Wu M., Isselbacher K. J., Bloch K. J. Intestinal uptake of macromolecules. III. Studies on the mechanism by which immunization interferes with antigen uptake. J Immunol. 1975 Sep;115(3):854–861. [PubMed] [Google Scholar]

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