Abstract
Using horseradish peroxidase and fluorescence immunohistochemistry, alpha 1-antitrypsin (alpha 1AT) was demonstrated in normal mouse hepatocytes and pancreatic islet cells. All hepatocytes were positive; 1--3% stained intensely for alpha 1AT. These were located mainly in the periportal area as well as randomly distributed, both singly and in clusters, throughout the liver lobule. Nonparenchymal liver cells were negative for alpha 1AT. The type of hepatocyte cytoplasmic staining appears to after during ontogeny, changing from a localized granular to a diffuse pattern. The use of immunohistochemistry to demonstrate alpha 1AT in normal mouse liver allows us to examine the acute phase response at a cellular level.
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Selected References
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- Bendayan M., Ito S. Immunohistochemical localization of exocrine enzymes in normal rat pancreas. J Histochem Cytochem. 1979 Jun;27(6):1029–1034. doi: 10.1177/27.6.110872. [DOI] [PubMed] [Google Scholar]
- Benítez-Bribiesca L., Freyre-Horta R. Immunofluorescent localization of alpha-1-antitrypsin in human polymorphonuclear leukocytes. Life Sci. 1978 Jan;22(1):99–104. doi: 10.1016/0024-3205(78)90417-4. [DOI] [PubMed] [Google Scholar]
- FORMAN W. B., BARNHART M. I. CELLULAR SITE FOR FIBRINOGEN SYNTHESIS. JAMA. 1964 Jan 11;187:128–132. [PubMed] [Google Scholar]
- Hood J. M., Koep L. J., Peters R. L., Schröter G. P., Weil R., 3rd, Redeker A. G., Starzl T. E. Liver transplantation for advanced liver disease with alpha-1-antitrypsin deficiency. N Engl J Med. 1980 Jan 31;302(5):272–275. doi: 10.1056/NEJM198001313020505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ishibashi H., Shibata K., Okubo H., Tsuda-Kawamura K., Yanase Y. Distribution of alpha 1-antitrypsin in normal, granuloma, and tumor tissues in rats. J Lab Clin Med. 1978 Apr;91(4):576–583. [PubMed] [Google Scholar]
- Koj A., Regoeczi E., Toews C. J., Leveille R., Gauldie J. Synthesis of antithrombin III and alpha-1-antitrypsin by the perfused rat liver. Biochim Biophys Acta. 1978 Apr 3;539(4):496–504. doi: 10.1016/0304-4165(78)90083-1. [DOI] [PubMed] [Google Scholar]
- Kuhlmann W. D. Purification of mouse alpha1-fetoprotein and preparation of specific peroxidase conjugates for its cellular localization. Histochemistry. 1975 Jul 30;44(2):155–167. doi: 10.1007/BF00494077. [DOI] [PubMed] [Google Scholar]
- Kushner I., Feldmann G. Control of the acute phase response. Demonstration of C-reactive protein synthesis and secretion by hepatocytes during acute inflammation in the rabbit. J Exp Med. 1978 Aug 1;148(2):466–477. doi: 10.1084/jem.148.2.466. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lipsky J. J., Berninger R. W., Hyman L. R., Talamo R. C. Presence of alpha-1-antitrypsin on mitogen-stimulated human lymphocytes. J Immunol. 1979 Jan;122(1):24–26. [PubMed] [Google Scholar]
- McElrath M. J., Galbraith R. M., Allen R. C. Demonstration of alpha 1-antitrypsin by immunofluorescence on paraffin-embedded hepatic and pancreatic tissue. J Histochem Cytochem. 1979 Mar;27(3):794–796. doi: 10.1177/27.3.383819. [DOI] [PubMed] [Google Scholar]
- Mihas A. A., Hirschowitz B. I. Alpha-antitrypsin and chronic pancreatitis. Lancet. 1976 Nov 6;2(7993):1032–1033. doi: 10.1016/s0140-6736(76)90883-7. [DOI] [PubMed] [Google Scholar]
- Myerowitz R. L., Chrambach A., Rodbard D., Robbins J. B. Isolation and characterization of mouse serum alpha 1-antitrypsins. Anal Biochem. 1972 Aug;48(2):394–409. doi: 10.1016/0003-2697(72)90092-9. [DOI] [PubMed] [Google Scholar]
- Nachman R. L., Harpel P. C. Platelet alpha2-macroglobulin and alpha1-antitrypsin. J Biol Chem. 1976 Aug 10;251(15):4512–4521. [PubMed] [Google Scholar]
- Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
- Nalli G., Cattaneo G., Malamani G. D., Majolino I., Fornasari P. M., Almasio P., Piovella F., Ascari E. Immunofluorescent detection of alpha 1-antitrypsin in platelets and megakaryocytes. Thromb Res. 1977 Apr;10(4):613–617. doi: 10.1016/0049-3848(77)90216-x. [DOI] [PubMed] [Google Scholar]
- Niewiarowski S. Proteins secreted by the platelet. Thromb Haemost. 1977 Dec 15;38(4):924–938. [PubMed] [Google Scholar]
- Palmer P. E., Christopherson W. M., Wolfe H. J. Alpha1-antitrypsin, protein marker in oral contraceptive-associated hepatic tumors. Am J Clin Pathol. 1977 Dec;68(6):736–739. doi: 10.1093/ajcp/68.6.736. [DOI] [PubMed] [Google Scholar]
- Palmer P. E., DeLellis R. A., Wolfe H. J. Immunohistochemistry of liver in alpha1-antitrypsin deficiency. A comparative study. Am J Clin Pathol. 1974 Sep;62(3):350–354. doi: 10.1093/ajcp/62.3.350. [DOI] [PubMed] [Google Scholar]
- Palmer P. E., Wolfe H. J. Alpha-antitrypsin deposition in primary hepatic carcinomas. Arch Pathol Lab Med. 1976 May;100(5):232–236. [PubMed] [Google Scholar]
- Peters J. H., Alper C. A. Haptoglobin synthesis. II. Cellular localization studies. J Clin Invest. 1966 Mar;45(3):314–320. doi: 10.1172/JCI105345. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peyrol S., Grimaud J. A., Pirson Y., Chayvialle J. A., Touillon C., Lambert B. Ultrastructural immunoenzymatic study of alpha-fetoprotein-producing cells in the human fetal liver. J Histochem Cytochem. 1977 Jun;25(6):432–438. doi: 10.1177/25.6.69654. [DOI] [PubMed] [Google Scholar]
- Rappaport A. M., Schneiderman J. H. The function of the hepatic artery. Rev Physiol Biochem Pharmacol. 1976;76:129–175. doi: 10.1007/BFb0027689. [DOI] [PubMed] [Google Scholar]
- Ray M. B., Desmet V. J. Immunofluorescent detection of alpha1-antitrypsin in paraffin embedded liver tissue. J Clin Pathol. 1975 Sep;28(9):717–721. doi: 10.1136/jcp.28.9.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ray M. B., Desmet V. J. Immunohistochemical demonstration of alpha-1-antitrypsin in the islet cells of human pancreas. Cell Tissue Res. 1978 Feb 14;187(1):69–77. doi: 10.1007/BF00220619. [DOI] [PubMed] [Google Scholar]
- Reintoft I., Hägerstrand I. Demonstration of alpha 1-antitrypsin in hepatomas. Arch Pathol Lab Med. 1979 Sep;103(10):495–498. [PubMed] [Google Scholar]
- Zehr D. R. Use of hydrogen peroxide-egg albumin to eliminate nonspecific staining in immunoperoxidase techniques. J Histochem Cytochem. 1978 May;26(5):415–416. doi: 10.1177/26.5.77872. [DOI] [PubMed] [Google Scholar]