Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1973 Aug;72(2):141–148.

Radiation Nephritis

III. Chemical, Functional and Morphologic Correlates of the Glomerular Basement Membrane

N F Gang, M E Sarophim, A Madrazo, J Churg
PMCID: PMC1903993  PMID: 4723396

Abstract

In the rat, radiation injury to a kidney results in severe glomerular lesions, with only a moderate increase in proteinuria. The proteinuria, however, was found to be nonselective. There was no relationship between the degree and selectivity of the proteinuria. No change in the gross chemical composition of the glomerular basement membrane obtained from the irradiated kidney was observed when compared with the GBM of a) untreated control kidneys, b) kidneys obtained from sham operated rats, c) unilaterally nephrectomized and d) control kidneys of irradiated animals. In conclusion, no correlation could be established between the morphologic alterations of the glomerulus and the chemical or permeability characteristics of the membrane.

Full text

PDF
141

Images in this article

147Fig 1
on p.147
147Fig 2
on p.147

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. CESSI C., PILIEGO F. The determination of amino sugars in the presence of amino acids and glucose. Biochem J. 1960 Dec;77:508–510. doi: 10.1042/bj0770508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. FRENK S., ANTONOWICZ I., CRAIG J. M., METCOFF J. Experimental nephrotic syndrome induced in rats by aminonucleoside; renal lesions and body electrolyte composition. Proc Soc Exp Biol Med. 1955 Jul;89(3):424–427. doi: 10.3181/00379727-89-21833. [DOI] [PubMed] [Google Scholar]
  6. Gang N. F., Kalant N. Nephrotoxic serum nephritis. I. Chemical, morphologic, and functional changes in the glomerular basement membrane during the evolution of nephritis. Lab Invest. 1970 Jun;22(6):531–540. [PubMed] [Google Scholar]
  7. Kefalides N. A., Forsell-Knott L. Structural changes in the protein and carbohydrate components of glomerular basement membrane in aminonucleoside nephrosis. Biochim Biophys Acta. 1970 Mar 17;203(1):62–66. doi: 10.1016/0005-2736(70)90035-0. [DOI] [PubMed] [Google Scholar]
  8. MONTGOMERY R. Further studies of the phenol-sulfuric acid reagent for carbohydrates. Biochim Biophys Acta. 1961 Apr 15;48:591–593. doi: 10.1016/0006-3002(61)90059-2. [DOI] [PubMed] [Google Scholar]
  9. Madrazo A., Suzuki Y., Churg J. Radiation nephritis. II. Chronic changes after high doses of radiation. Am J Pathol. 1970 Oct;61(1):37–56. [PMC free article] [PubMed] [Google Scholar]
  10. Madrazo A., Suzuki Y., Churg J. Radiation nephritis: acute changes following high dose of radiation. Am J Pathol. 1969 Mar;54(3):507–527. [PMC free article] [PubMed] [Google Scholar]
  11. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES