Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1996 Aug;70(8):5061–5066. doi: 10.1128/jvi.70.8.5061-5066.1996

Protection against myxomatosis and rabbit viral hemorrhagic disease with recombinant myxoma viruses expressing rabbit hemorrhagic disease virus capsid protein.

S Bertagnoli 1, J Gelfi 1, G Le Gall 1, E Boilletot 1, J F Vautherot 1, D Rasschaert 1, S Laurent 1, F Petit 1, C Boucraut-Baralon 1, A Milon 1
PMCID: PMC190460  PMID: 8764013

Abstract

Two myxoma virus-rabbit hemorrhagic disease virus (RHDV) recombinant viruses were constructed with the SG33 strain of myxoma virus to protect rabbits against myxomatosis and rabbit viral hemorrhagic disease. These recombinant viruses expressed the RHDV capsid protein (VP60). The recombinant protein, which is 60 kDa in size, was antigenic, as revealed by its reaction in immunoprecipitation with antibodies raised against RHDV. Both recombinant viruses induced high levels of RHDV- and myxoma virus-specific antibodies in rabbits after immunization. Inoculations by the intradermal route protected animals against virulent RHDV and myxoma virus challenges.

Full Text

The Full Text of this article is available as a PDF (243.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Argüello Villares J. L. Viral haemorrhagic disease of rabbits: vaccination and immune response. Rev Sci Tech. 1991 Jun;10(2):459–480. [PubMed] [Google Scholar]
  2. Blancou J., Kieny M. P., Lathe R., Lecocq J. P., Pastoret P. P., Soulebot J. P., Desmettre P. Oral vaccination of the fox against rabies using a live recombinant vaccinia virus. Nature. 1986 Jul 24;322(6077):373–375. doi: 10.1038/322373a0. [DOI] [PubMed] [Google Scholar]
  3. Boga J. A., Casais R., Marin M. S., Martin-Alonso J. M., Carmenes R. S., Prieto M., Parra F. Molecular cloning, sequencing and expression in Escherichia coli of the capsid protein gene from rabbit haemorrhagic disease virus (Spanish isolate AST/89). J Gen Virol. 1994 Sep;75(Pt 9):2409–2413. doi: 10.1099/0022-1317-75-9-2409. [DOI] [PubMed] [Google Scholar]
  4. Brochier B., Kieny M. P., Costy F., Coppens P., Bauduin B., Lecocq J. P., Languet B., Chappuis G., Desmettre P., Afiademanyo K. Large-scale eradication of rabies using recombinant vaccinia-rabies vaccine. Nature. 1991 Dec 19;354(6354):520–522. doi: 10.1038/354520a0. [DOI] [PubMed] [Google Scholar]
  5. Buller R. M., Smith G. L., Cremer K., Notkins A. L., Moss B. Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype. 1985 Oct 31-Nov 6Nature. 317(6040):813–815. doi: 10.1038/317813a0. [DOI] [PubMed] [Google Scholar]
  6. Chakrabarti S., Brechling K., Moss B. Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques. Mol Cell Biol. 1985 Dec;5(12):3403–3409. doi: 10.1128/mcb.5.12.3403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Drillien R., Spehner D., Kirn A., Giraudon P., Buckland R., Wild F., Lecocq J. P. Protection of mice from fatal measles encephalitis by vaccination with vaccinia virus recombinants encoding either the hemagglutinin or the fusion protein. Proc Natl Acad Sci U S A. 1988 Feb;85(4):1252–1256. doi: 10.1073/pnas.85.4.1252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Giavedoni L., Jones L., Mebus C., Yilma T. A vaccinia virus double recombinant expressing the F and H genes of rinderpest virus protects cattle against rinderpest and causes no pock lesions. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8011–8015. doi: 10.1073/pnas.88.18.8011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Graham K. A., Opgenorth A., Upton C., McFadden G. Myxoma virus M11L ORF encodes a protein for which cell surface localization is critical in manifestation of viral virulence. Virology. 1992 Nov;191(1):112–124. doi: 10.1016/0042-6822(92)90172-l. [DOI] [PubMed] [Google Scholar]
  10. Jackson R. J., Bults H. G. The myxoma virus thymidine kinase gene: sequence and transcriptional mapping. J Gen Virol. 1992 Feb;73(Pt 2):323–328. doi: 10.1099/0022-1317-73-2-323. [DOI] [PubMed] [Google Scholar]
  11. Jones L., Giavedoni L., Saliki J. T., Brown C., Mebus C., Yilma T. Protection of goats against peste des petits ruminants with a vaccinia virus double recombinant expressing the F and H genes of rinderpest virus. Vaccine. 1993;11(9):961–964. doi: 10.1016/0264-410x(93)90386-c. [DOI] [PubMed] [Google Scholar]
  12. Kieny M. P., Lathe R., Drillien R., Spehner D., Skory S., Schmitt D., Wiktor T., Koprowski H., Lecocq J. P. Expression of rabies virus glycoprotein from a recombinant vaccinia virus. Nature. 1984 Nov 8;312(5990):163–166. doi: 10.1038/312163a0. [DOI] [PubMed] [Google Scholar]
  13. Laurent S., Vautherot J. F., Madelaine M. F., Le Gall G., Rasschaert D. Recombinant rabbit hemorrhagic disease virus capsid protein expressed in baculovirus self-assembles into viruslike particles and induces protection. J Virol. 1994 Oct;68(10):6794–6798. doi: 10.1128/jvi.68.10.6794-6798.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marcato P. S., Benazzi C., Vecchi G., Galeotti M., Della Salda L., Sarli G., Lucidi P. Clinical and pathological features of viral haemorrhagic disease of rabbits and the European brown hare syndrome. Rev Sci Tech. 1991 Jun;10(2):371–392. doi: 10.20506/rst.10.2.560. [DOI] [PubMed] [Google Scholar]
  15. Meyers G., Wirblich C., Thiel H. J. Rabbit hemorrhagic disease virus--molecular cloning and nucleotide sequencing of a calicivirus genome. Virology. 1991 Oct;184(2):664–676. doi: 10.1016/0042-6822(91)90436-f. [DOI] [PubMed] [Google Scholar]
  16. Moss B., Smith G. L., Gerin J. L., Purcell R. H. Live recombinant vaccinia virus protects chimpanzees against hepatitis B. Nature. 1984 Sep 6;311(5981):67–69. doi: 10.1038/311067a0. [DOI] [PubMed] [Google Scholar]
  17. Ohlinger V. F., Haas B., Meyers G., Weiland F., Thiel H. J. Identification and characterization of the virus causing rabbit hemorrhagic disease. J Virol. 1990 Jul;64(7):3331–3336. doi: 10.1128/jvi.64.7.3331-3336.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Opgenorth A., Graham K., Nation N., Strayer D., McFadden G. Deletion analysis of two tandemly arranged virulence genes in myxoma virus, M11L and myxoma growth factor. J Virol. 1992 Aug;66(8):4720–4731. doi: 10.1128/jvi.66.8.4720-4731.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Panicali D., Paoletti E. Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4927–4931. doi: 10.1073/pnas.79.16.4927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Parra F., Prieto M. Purification and characterization of a calicivirus as the causative agent of a lethal hemorrhagic disease in rabbits. J Virol. 1990 Aug;64(8):4013–4015. doi: 10.1128/jvi.64.8.4013-4015.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rasschaert D., Huguet S., Madelaine M. F., Vautherot J. F. Sequence and genomic organization of a rabbit hemorrhagic disease virus isolated from a wild rabbit. Virus Genes. 1995 Jan;9(2):121–132. doi: 10.1007/BF01702655. [DOI] [PubMed] [Google Scholar]
  22. Rodák L., Smíd B., Valícek L., Veselý T., Stepánek J., Hampl J., Jurák E. Enzyme-linked immunosorbent assay of antibodies to rabbit haemorrhagic disease virus and determination of its major structural proteins. J Gen Virol. 1990 May;71(Pt 5):1075–1080. doi: 10.1099/0022-1317-71-5-1075. [DOI] [PubMed] [Google Scholar]
  23. Romero C. H., Barrett T., Evans S. A., Kitching R. P., Gershon P. D., Bostock C., Black D. N. Single capripoxvirus recombinant vaccine for the protection of cattle against rinderpest and lumpy skin disease. Vaccine. 1993;11(7):737–742. doi: 10.1016/0264-410x(93)90258-y. [DOI] [PubMed] [Google Scholar]
  24. Russell R. J., Robbins S. J. Cloning and molecular characterization of the myxoma virus genome. Virology. 1989 May;170(1):147–159. doi: 10.1016/0042-6822(89)90362-0. [DOI] [PubMed] [Google Scholar]
  25. Smith G. L., Murphy B. R., Moss B. Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters. Proc Natl Acad Sci U S A. 1983 Dec;80(23):7155–7159. doi: 10.1073/pnas.80.23.7155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Upton C., Macen J. L., McFadden G. Mapping and sequencing of a gene from myxoma virus that is related to those encoding epidermal growth factor and transforming growth factor alpha. J Virol. 1987 Apr;61(4):1271–1275. doi: 10.1128/jvi.61.4.1271-1275.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wiktor T. J., Macfarlan R. I., Reagan K. J., Dietzschold B., Curtis P. J., Wunner W. H., Kieny M. P., Lathe R., Lecocq J. P., Mackett M. Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7194–7198. doi: 10.1073/pnas.81.22.7194. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES