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. 1996 Aug;70(8):5646–5650. doi: 10.1128/jvi.70.8.5646-5650.1996

Simian immunodeficiency virus infection of CD8+ lymphocytes in vivo.

G A Dean 1, G H Reubel 1, N C Pedersen 1
PMCID: PMC190527  PMID: 8764081

Abstract

To determine the lymphoid target cells of simian immunodeficiency virus (SIV) in vivo, peripheral blood lymphocytes (PBL) and lymph node lymphocytes (LNL) were positively selected (>97% purity) for surface expression of CD4, CD8, or CD20 and then analyzed for SIV provirus using semiquantitative DNA amplification. We found provirus in CD4+ and CD8+ lymphocytes but none in CD20+ lymphocytes. During acute SIV infection (< or = 214 days postinoculation), the percentage of PBL and LNL CD4+ cells containing proviral DNA ranged from 0.2 to 20% and from 0.2 to 2%, respectively. Proviral burden in the CD8+ population of either PBL or LNL ranged from 0.01 to 0.2%. Virus isolation by cocultivation was positive for both CD4+ and CD8+ purified populations. No difference in proviral burden was observed between PBL and LNL subsets during acute SIV infection. Up to 19.4% of positively selected CD8+ cells also expressed CD4, and thus the provirus may reside within a dual-positive population. This dual-positive population may represent activated lymphocytes that are particularly susceptible to infection and may provide an opportunity for virus entry into the CD8+ CD4- lymphocytes in vivo.

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Selected References

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  1. Blue M. L., Daley J. F., Levine H., Craig K. A., Schlossman S. F. Biosynthesis and surface expression of T8 by peripheral blood T4+ cells in vitro. J Immunol. 1986 Aug 15;137(4):1202–1207. [PubMed] [Google Scholar]
  2. Blue M. L., Daley J. F., Levine H., Schlossman S. F. Coexpression of T4 and T8 on peripheral blood T cells demonstrated by two-color fluorescence flow cytometry. J Immunol. 1985 Apr;134(4):2281–2286. [PubMed] [Google Scholar]
  3. Brinchmann J. E., Albert J., Vartdal F. Few infected CD4+ T cells but a high proportion of replication-competent provirus copies in asymptomatic human immunodeficiency virus type 1 infection. J Virol. 1991 Apr;65(4):2019–2023. doi: 10.1128/jvi.65.4.2019-2023.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Farcet J. P., Gourdin M. F., Calvo C., Oudrhiri N., Divine M., Bouguet J., Fradelizzi D., Senik A., Reyes F. A subset of OKT4+ peripheral T cells can generate colonies containing mixed progeny with OKT4+ helper and OKT8+ suppressor cells. Eur J Immunol. 1985 Oct;15(10):1067–1073. doi: 10.1002/eji.1830151020. [DOI] [PubMed] [Google Scholar]
  5. Gallatin W. M., Gale M. J., Jr, Hoffman P. A., Willerford D. M., Draves K. E., Benveniste R. E., Morton W. R., Clark E. A. Selective replication of simian immunodeficiency virus in a subset of CD4+ lymphocytes. Proc Natl Acad Sci U S A. 1989 May;86(9):3301–3305. doi: 10.1073/pnas.86.9.3301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graziosi C., Pantaleo G., Demarest J. F., Cohen O. J., Vaccarezza M., Butini L., Montroni M., Fauci A. S. HIV-1 infection in the lymphoid organs. AIDS. 1993 Nov;7 (Suppl 2):S53–S58. doi: 10.1097/00002030-199311002-00012. [DOI] [PubMed] [Google Scholar]
  7. Hoxie J. A., Haggarty B. S., Bonser S. E., Rackowski J. L., Shan H., Kanki P. J. Biological characterization of a simian immunodeficiency virus-like retrovirus (HTLV-IV): evidence for CD4-associated molecules required for infection. J Virol. 1988 Aug;62(8):2557–2568. doi: 10.1128/jvi.62.8.2557-2568.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hufert F. T., von Laer D., Schramm C., Tárnok A., Schmitz H. Detection of HIV-1 DNA in different subsets of human peripheral blood mononuclear cells using the polymerase chain reaction. Rapid communication. Arch Virol. 1989;106(3-4):341–345. doi: 10.1007/BF01313963. [DOI] [PubMed] [Google Scholar]
  9. Koenig S., Hirsch V. M., Olmsted R. A., Powell D., Maury W., Rabson A., Fauci A. S., Purcell R. H., Johnson P. R. Selective infection of human CD4+ cells by simian immunodeficiency virus: productive infection associated with envelope glycoprotein-induced fusion. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2443–2447. doi: 10.1073/pnas.86.7.2443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Macchi B., Graziani G., Zhang J., Mastino A. Emergence of double-positive CD4/CD8 cells from adult peripheral blood mononuclear cells infected with human T cell leukemia virus type I (HTLV-I). Cell Immunol. 1993 Jul;149(2):376–389. doi: 10.1006/cimm.1993.1163. [DOI] [PubMed] [Google Scholar]
  11. Miller C. J., Marthas M., Torten J., Alexander N. J., Moore J. P., Doncel G. F., Hendrickx A. G. Intravaginal inoculation of rhesus macaques with cell-free simian immunodeficiency virus results in persistent or transient viremia. J Virol. 1994 Oct;68(10):6391–6400. doi: 10.1128/jvi.68.10.6391-6400.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Montaner L. J., Ringler D. J., Wyand M. S., Mattmuller M. R., MacKey J. J., Schmidt D. K., Daniel M. D., King N. W. Study of long-term cultures of simian immunodeficiency virus (SIVmac 251)-infected peripheral blood lymphocytes. Lab Invest. 1990 Aug;63(2):242–247. [PubMed] [Google Scholar]
  13. Ortolani C., Forti E., Radin E., Cibin R., Cossarizza A. Cytofluorimetric identification of two populations of double positive (CD4+,CD8+) T lymphocytes in human peripheral blood. Biochem Biophys Res Commun. 1993 Mar 15;191(2):601–609. doi: 10.1006/bbrc.1993.1260. [DOI] [PubMed] [Google Scholar]
  14. Pantaleo G., Graziosi C., Butini L., Pizzo P. A., Schnittman S. M., Kotler D. P., Fauci A. S. Lymphoid organs function as major reservoirs for human immunodeficiency virus. Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9838–9842. doi: 10.1073/pnas.88.21.9838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Psallidopoulos M. C., Schnittman S. M., Thompson L. M., 3rd, Baseler M., Fauci A. S., Lane H. C., Salzman N. P. Integrated proviral human immunodeficiency virus type 1 is present in CD4+ peripheral blood lymphocytes in healthy seropositive individuals. J Virol. 1989 Nov;63(11):4626–4631. doi: 10.1128/jvi.63.11.4626-4631.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reimann K. A., Waite B. C., Lee-Parritz D. E., Lin W., Uchanska-Ziegler B., O'Connell M. J., Letvin N. L. Use of human leukocyte-specific monoclonal antibodies for clinically immunophenotyping lymphocytes of rhesus monkeys. Cytometry. 1994 Sep 1;17(1):102–108. doi: 10.1002/cyto.990170113. [DOI] [PubMed] [Google Scholar]
  17. Schnittman S. M., Greenhouse J. J., Psallidopoulos M. C., Baseler M., Salzman N. P., Fauci A. S., Lane H. C. Increasing viral burden in CD4+ T cells from patients with human immunodeficiency virus (HIV) infection reflects rapidly progressive immunosuppression and clinical disease. Ann Intern Med. 1990 Sep 15;113(6):438–443. doi: 10.7326/0003-4819-113-6-438. [DOI] [PubMed] [Google Scholar]
  18. Schnittman S. M., Psallidopoulos M. C., Lane H. C., Thompson L., Baseler M., Massari F., Fox C. H., Salzman N. P., Fauci A. S. The reservoir for HIV-1 in human peripheral blood is a T cell that maintains expression of CD4. Science. 1989 Jul 21;245(4915):305–308. doi: 10.1126/science.2665081. [DOI] [PubMed] [Google Scholar]
  19. Schwiebert R., Fultz P. N. Immune activation and viral burden in acute disease induced by simian immunodeficiency virus SIVsmmPBj14: correlation between in vitro and in vivo events. J Virol. 1994 Sep;68(9):5538–5547. doi: 10.1128/jvi.68.9.5538-5547.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stone J. D., Heise C. C., Miller C. J., Halsted C. H., Dandekar S. Development of malabsorption and nutritional complications in simian immunodeficiency virus-infected rhesus macaques. AIDS. 1994 Sep;8(9):1245–1256. doi: 10.1097/00002030-199409000-00005. [DOI] [PubMed] [Google Scholar]
  21. Tsubota H., Ringler D. J., Kannagi M., King N. W., Solomon K. R., MacKey J. J., Walsh D. G., Letvin N. L. CD8+CD4- lymphocyte lines can harbor the AIDS virus in vitro. J Immunol. 1989 Aug 1;143(3):858–863. [PubMed] [Google Scholar]
  22. Unger R. E., Marthas M. L., Lackner A. A., Pratt-Lowe E., Lohman B. L., Van Rompay K., Luciw P. A. Detection of simian immunodeficiency virus DNA in macrophages from infected rhesus macaques. J Med Primatol. 1992 Feb-May;21(2-3):74–81. [PubMed] [Google Scholar]
  23. Willerford D. M., Gale M. J., Jr, Benveniste R. E., Clark E. A., Gallatin W. M. Simian immunodeficiency virus is restricted to a subset of blood CD4+ lymphocytes that includes memory cells. J Immunol. 1990 May 15;144(10):3779–3783. [PubMed] [Google Scholar]

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