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. 1996 Aug;70(8):5716–5719. doi: 10.1128/jvi.70.8.5716-5719.1996

Evidence for a phosphorylation site in cytomegalovirus glycoprotein gB.

N Norais 1, J A Hall 1, L Gross 1, D Tang 1, S Kaur 1, S H Chamberlain 1, R L Burke 1, F Marcus 1
PMCID: PMC190541  PMID: 8764095

Abstract

As part of our vaccine program, we have purified a recombinant form of human cytomegalovirus glycoprotein B that is able to induce high titers of virus-neutralizing antibodies. The isolated protein was found to be phosphorylated at a serine residue in position -7 from the C terminus of the protein. The corresponding synthetic peptide, HLKDSDEEENV, was an efficient in vitro substrate of casein kinase II.

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Selected References

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  1. Allende J. E., Allende C. C. Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation. FASEB J. 1995 Mar;9(5):313–323. doi: 10.1096/fasebj.9.5.7896000. [DOI] [PubMed] [Google Scholar]
  2. Biemann K. Appendix 5. Nomenclature for peptide fragment ions (positive ions). Methods Enzymol. 1990;193:886–887. doi: 10.1016/0076-6879(90)93460-3. [DOI] [PubMed] [Google Scholar]
  3. Britt W. J., Vugler L., Butfiloski E. J., Stephens E. B. Cell surface expression of human cytomegalovirus (HCMV) gp55-116 (gB): use of HCMV-recombinant vaccinia virus-infected cells in analysis of the human neutralizing antibody response. J Virol. 1990 Mar;64(3):1079–1085. doi: 10.1128/jvi.64.3.1079-1085.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fenn J. B., Mann M., Meng C. K., Wong S. F., Whitehouse C. M. Electrospray ionization for mass spectrometry of large biomolecules. Science. 1989 Oct 6;246(4926):64–71. doi: 10.1126/science.2675315. [DOI] [PubMed] [Google Scholar]
  5. Hunt D. F., Yates J. R., 3rd, Shabanowitz J., Winston S., Hauer C. R. Protein sequencing by tandem mass spectrometry. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6233–6237. doi: 10.1073/pnas.83.17.6233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kongsuwan K., Prideaux C. T., Johnson M. A., Sheppard M., Fahey K. J. Nucleotide sequence of the gene encoding infectious laryngotracheitis virus glycoprotein B. Virology. 1991 Sep;184(1):404–410. doi: 10.1016/0042-6822(91)90859-a. [DOI] [PubMed] [Google Scholar]
  7. Kuenzel E. A., Mulligan J. A., Sommercorn J., Krebs E. G. Substrate specificity determinants for casein kinase II as deduced from studies with synthetic peptides. J Biol Chem. 1987 Jul 5;262(19):9136–9140. [PubMed] [Google Scholar]
  8. Marcus F., Rittenhouse J., Moberly L., Edelstein I., Hiller E., Rogers D. T. Yeast (Saccharomyces cerevisiae) fructose-1,6-bisphosphatase. Properties of phospho and dephospho forms and of two mutants in which serine 11 has been changed by site-directed mutagenesis. J Biol Chem. 1988 May 5;263(13):6058–6062. [PubMed] [Google Scholar]
  9. Navarro D., Paz P., Tugizov S., Topp K., La Vail J., Pereira L. Glycoprotein B of human cytomegalovirus promotes virion penetration into cells, transmission of infection from cell to cell, and fusion of infected cells. Virology. 1993 Nov;197(1):143–158. doi: 10.1006/viro.1993.1575. [DOI] [PubMed] [Google Scholar]
  10. Oray B., Jahani M., Gracy R. W. High-sensitivity peptide mapping of triosephosphate isomerase: a comparison of high-performance liquid chromatography with two-dimensional thin-layer methods. Anal Biochem. 1982 Sep 1;125(1):131–138. doi: 10.1016/0003-2697(82)90393-1. [DOI] [PubMed] [Google Scholar]
  11. Pinna L. A. Casein kinase 2: an 'eminence grise' in cellular regulation? Biochim Biophys Acta. 1990 Sep 24;1054(3):267–284. doi: 10.1016/0167-4889(90)90098-x. [DOI] [PubMed] [Google Scholar]
  12. Rapp M., Messerle M., Bühler B., Tannheimer M., Keil G. M., Koszinowski U. H. Identification of the murine cytomegalovirus glycoprotein B gene and its expression by recombinant vaccinia virus. J Virol. 1992 Jul;66(7):4399–4406. doi: 10.1128/jvi.66.7.4399-4406.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ross L. J., Sanderson M., Scott S. D., Binns M. M., Doel T., Milne B. Nucleotide sequence and characterization of the Marek's disease virus homologue of glycoprotein B of herpes simplex virus. J Gen Virol. 1989 Jul;70(Pt 7):1789–1804. doi: 10.1099/0022-1317-70-7-1789. [DOI] [PubMed] [Google Scholar]
  14. Spaete R. R. A recombinant subunit vaccine approach to HCMV vaccine development. Transplant Proc. 1991 Jun;23(3 Suppl 3):90–96. [PubMed] [Google Scholar]
  15. Spaete R. R., Saxena A., Scott P. I., Song G. J., Probert W. S., Britt W. J., Gibson W., Rasmussen L., Pachl C. Sequence requirements for proteolytic processing of glycoprotein B of human cytomegalovirus strain Towne. J Virol. 1990 Jun;64(6):2922–2931. doi: 10.1128/jvi.64.6.2922-2931.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spaete R. R., Thayer R. M., Probert W. S., Masiarz F. R., Chamberlain S. H., Rasmussen L., Merigan T. C., Pachl C. Human cytomegalovirus strain Towne glycoprotein B is processed by proteolytic cleavage. Virology. 1988 Nov;167(1):207–225. doi: 10.1016/0042-6822(88)90071-2. [DOI] [PubMed] [Google Scholar]
  17. Tugizov S., Wang Y., Qadri I., Navarro D., Maidji E., Pereira L. Mutated forms of human cytomegalovirus glycoprotein B are impaired in inducing syncytium formation. Virology. 1995 Jun 1;209(2):580–591. doi: 10.1006/viro.1995.1290. [DOI] [PubMed] [Google Scholar]
  18. Yow M. D., Demmler G. J. Congenital cytomegalovirus disease--20 years is long enough. N Engl J Med. 1992 Mar 5;326(10):702–703. doi: 10.1056/NEJM199203053261010. [DOI] [PubMed] [Google Scholar]
  19. Zaia J. A. Pathogenesis of CMV-associated diseases in 1990. Transplant Proc. 1991 Jun;23(3 Suppl 3):1–4. [PubMed] [Google Scholar]

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