Abstract
The effect of human cytomegalovirus infection on cellular DNA synthesis in human fibroblasts was measured by fluorometry and by incorporation of radiolabeled thymidine. The results show that although HCMV infection stimulates cellular DNA synthesis in both quiescent and serum-stimulated cells, radiolabeled thymidine is almost exclusively incorporated into viral DNA.
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- Albrecht T., Boldogh I., Fons M., Lee C. H., AbuBakar S., Russell J. M., Au W. W. Cell-activation responses to cytomegalovirus infection relationship to the phasing of CMV replication and to the induction of cellular damage. Subcell Biochem. 1989;15:157–202. [PubMed] [Google Scholar]
- Ball R. L., Carney D. H., Albrecht T. Taxol inhibits stimulation of cell DNA synthesis by human cytomegalovirus. Exp Cell Res. 1990 Nov;191(1):37–44. doi: 10.1016/0014-4827(90)90032-6. [DOI] [PubMed] [Google Scholar]
- Biron K. K., Fyfe J. A., Stanat S. C., Leslie L. K., Sorrell J. B., Lambe C. U., Coen D. M. A human cytomegalovirus mutant resistant to the nucleoside analog 9-([2-hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (BW B759U) induces reduced levels of BW B759U triphosphate. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8769–8773. doi: 10.1073/pnas.83.22.8769. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brooks R. F. The kinetics of serum-induced initiation of DNA synthesis in BHK 21/C13 cells, and the influence of exogenous adenosine. J Cell Physiol. 1975 Oct;86(2 Pt 2 Suppl 1):369–377. doi: 10.1002/jcp.1040860409. [DOI] [PubMed] [Google Scholar]
- DeMarchi J. M. Correlation between stimulation of host cell DNA synthesis by human cytomegalovirus and lack of expression of a subset of early virus genes. Virology. 1983 Sep;129(2):274–286. doi: 10.1016/0042-6822(83)90167-8. [DOI] [PubMed] [Google Scholar]
- Estes J. E., Huang E. S. Stimulation of cellular thymidine kinases by human cytomegalovirus. J Virol. 1977 Oct;24(1):13–21. doi: 10.1128/jvi.24.1.13-21.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ihara S., Saito S., Watanabe Y. Effect of UV-irradiated human cytomegalovirus infection on exogenous thymidine metabolism in human embryonic lung cells. Variation depending cells. Microbiol Immunol. 1980;24(2):179–184. doi: 10.1111/j.1348-0421.1980.tb00576.x. [DOI] [PubMed] [Google Scholar]
- Ihara S., Saito S., Watanabe Y. Human cytomegalovirus-induced inhibition of exogenous thymidine uptake into cell DNA in HEL cells stimulated to proliferate with serum. Tokai J Exp Clin Med. 1980 Jul;5(3):301–309. [PubMed] [Google Scholar]
- Jault F. M., Jault J. M., Ruchti F., Fortunato E. A., Clark C., Corbeil J., Richman D. D., Spector D. H. Cytomegalovirus infection induces high levels of cyclins, phosphorylated Rb, and p53, leading to cell cycle arrest. J Virol. 1995 Nov;69(11):6697–6704. doi: 10.1128/jvi.69.11.6697-6704.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jeor S. C., Albrecht T. B., Funk F. D., Rapp F. Stimulation of cellular DNA synthesis by human cytomegalovirus. J Virol. 1974 Feb;13(2):353–362. doi: 10.1128/jvi.13.2.353-362.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sorger T., Germinario R. J. A direct solubilization procedure for the determination of DNA and protein in cultured fibroblast monolayers. Anal Biochem. 1983 May;131(1):254–256. doi: 10.1016/0003-2697(83)90163-x. [DOI] [PubMed] [Google Scholar]
- Temin H. M. Carcinogenesis by avian sarcoma viruses. X. The decreased requirement for insulin-replaceable activity in serum for cell multiplication. Int J Cancer. 1968 Nov 15;3(6):771–787. doi: 10.1002/ijc.2910030611. [DOI] [PubMed] [Google Scholar]
- Yamanishi K., Rapp F. Induction of host DNA synthesis and DNA polymerase by DNA-negative temperature-sensitive mutants of human cytomegalovirus. Virology. 1979 Apr 15;94(1):237–241. doi: 10.1016/0042-6822(79)90457-4. [DOI] [PubMed] [Google Scholar]
- Závada V., Erban V., Rezácová D., Vonka V. Thymidine-kinase in cytomegalovirus infected cells. Arch Virol. 1976;52(4):333–339. doi: 10.1007/BF01315622. [DOI] [PubMed] [Google Scholar]