Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1973 Apr;71(1):1–22.

Contractile Proteins of Endothelial Cells, Platelets and Smooth Muscle

Carl G Becker, Ralph L Nachman
PMCID: PMC1907211  PMID: 4267065

Abstract

In experiments described herein it was observed, by direct and indirect immunofluorescence technics, that rabbit antisera to human platelet actomyosin (thrombosthenin) stained mature megakaryocytes, blood platelets, endothelial cells and smooth muscle cells of arteries and veins, endothelial cells of liver sinusoids and certain capillaries, uterine smooth muscle cells, myoepithelial cells, perineurial cells of peripheral nerves and “fibroblastic” cells of granulation tissue. The specificity of immunohistologic staining was confirmed by appropriate absorption and blocking studies and immunodiffusional analysis in agarose gel. It was also observed by immunodiffusional analysis in agarose gel, electrophoresis of actomyosin fragments in polyacrylamide gels, immune inhibition of actomyosin ATPase activity and immune aggregation of platelets that uterine and platelet actomyosin are partially, but not completely, identical.

Full text

PDF
1

Images in this article

19Fig 1
on p.19
19Fig 2
on p.19
19Fig 3
on p.19
19Fig 4
on p.19
19Fig 5
on p.19
19Fig 6
on p.19
19Fig 7
on p.19
19Fig 8
on p.19
19Fig 9
on p.19
21Fig 10
on p.21
21Fig 11
on p.21

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURKHOLDER P. M., LITTELL A. H., KLEIN P. G. Sectioning at room temperature of unfixed tissues, frozen in a gelatin matrix, for immunohistologic procedures. Stain Technol. 1961 Mar;36:89–91. doi: 10.3109/10520296109113250. [DOI] [PubMed] [Google Scholar]
  2. Becker C. G. Demonstration of actomyosin in mesangial cells of the renal glomerulus. Am J Pathol. 1972 Jan;66(1):97–110. [PMC free article] [PubMed] [Google Scholar]
  3. Becker C. G., Murphy G. E. Demonstration of contractile protein in endothelium and cells of the heart valves, endocardium, intima, arteriosclerotic plaques, and Aschoff bodies of rheumatic heart disease. Am J Pathol. 1969 Apr;55(1):1–37. [PMC free article] [PubMed] [Google Scholar]
  4. Booyse F. M., Sternberger L. A., Zschocke D., Rafelson M. E., Jr Ultrastructural localization of contractile protein (thrombosthenin) in human platelets using an unlabeled antibody-peroxidase staining technique. J Histochem Cytochem. 1971 Sep;19(9):540–550. doi: 10.1177/19.9.540. [DOI] [PubMed] [Google Scholar]
  5. Chambers D. A., Salzman E. W., Neri L. L. Characterization of "ecto-ATPase" of human blood platelets. Arch Biochem Biophys. 1967 Mar;119(1):173–178. doi: 10.1016/0003-9861(67)90444-4. [DOI] [PubMed] [Google Scholar]
  6. Finck H. Immunochemical studies on myosin. II. Cardiac myosin. Biochim Biophys Acta. 1965 Nov 15;111(1):221–230. doi: 10.1016/0304-4165(65)90488-5. [DOI] [PubMed] [Google Scholar]
  7. Gabbiani G., Hirschel B. J., Ryan G. B., Statkov P. R., Majno G. Granulation tissue as a contractile organ. A study of structure and function. J Exp Med. 1972 Apr 1;135(4):719–734. doi: 10.1084/jem.135.4.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gimbrone M. A., Jr, Aster R. H., Cotran R. S., Corkery J., Jandl J. H., Folkman J. Preservation of vascular integrity in organs perfused in vitro with a platelet-rich medium. Nature. 1969 Apr 5;222(5188):33–36. doi: 10.1038/222033a0. [DOI] [PubMed] [Google Scholar]
  9. Gore I., Takada M., Austin J. Ultrastructural basis of experimental thrombocytopenic purpura. Arch Pathol. 1970 Sep;90(3):197–205. [PubMed] [Google Scholar]
  10. Karnovsky M. J. The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol. 1967 Oct;35(1):213–236. doi: 10.1083/jcb.35.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kemp R. B., Jones B. M., Gröschel-Stewart U. Aggregative behaviour of embryonic chick cells in the presence of antibodies directed against actomyosins. J Cell Sci. 1971 Jul;9(1):103–122. doi: 10.1242/jcs.9.1.103. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Majno G., Shea S. M., Leventhal M. Endothelial contraction induced by histamine-type mediators: an electron microscopic study. J Cell Biol. 1969 Sep;42(3):647–672. doi: 10.1083/jcb.42.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Markham R. A steam distillation apparatus suitable for micro-Kjeldahl analysis. Biochem J. 1942 Dec;36(10-12):790–791. doi: 10.1042/bj0360790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nachman R. L., Marcus A. J., Safier L. B. Platelet thrombosthenin: subcellular localization and function. J Clin Invest. 1967 Aug;46(8):1380–1389. doi: 10.1172/JCI105630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Roskam J. The hemostatic paradox and its present problems. Thromb Diath Haemorrh. 1965 Nov 15;14(3-4):626–639. [PubMed] [Google Scholar]
  17. Sarkar S., Sreter F. A., Gergely J. Light chains of myosins from white, red, and cardiac muscles. Proc Natl Acad Sci U S A. 1971 May;68(5):946–950. doi: 10.1073/pnas.68.5.946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wexler M. J., Ginsburg A. D., Latzina A., Aster R. A., Slapak M. Twenty-four hour renal preservation and perfusion utilizing platelet-rich plasma. Ann Surg. 1971 Nov;174(5):811–825. doi: 10.1097/00000658-197111000-00012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wojcik J. D., Van Horn D. L., Webber A. J., Johnson S. A. Mechanism whereby platelets support the endothelium. Transfusion. 1969 Nov-Dec;9(6):324–335. doi: 10.1111/j.1537-2995.1969.tb04945.x. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES